Creation of a prevascularized site for cell transplantation in rats

Stiegler, Philipp; Matzi, Veronika; Pierer, E.; Hauser, O.; Schaffellner, S.; Renner, Heiko; Greilberger, Joachim; Aigner, Reingard; Maier, Andrea B.; Lackner, Carolin; Iberer, F.; Smolle‐Jüttner, Freyja‐Maria; Tscheliessnigg, Karlheinz; Stadlbauer, Vanessa

doi:10.1111/j.1399-3089.2010.00606.x

Cited by 22 publications

(12 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the fact that there is still no reliable method to generate insulin producing cells from stem/progenitor cells [9,[24][25][26] improving the efficacy of islet transplantation, especially focusing on porcine islet xenotransplantation seems the most realistic and prudent method to cure diabetes [3]. The improvement in islet isolation outcomes and viability after isolation, purification, and in the case of xenotransplantation, after microencapsulation results from innovations such as the procurement and the preservation of the pancreatic tissue [24].…”

Section: Discussionmentioning

confidence: 99%

“…It is conceivable that the harvested pancreatic tissue suffers irreversible damage due to the high susceptibility against hypoxic conditions [1][2][3] following prolonged cold ischemic time (CIT) due to isolation, purification and microencapsulation processes diminishing islet cell yield and viability. The consequence is a reduced ability to reverse diabetes after xenotransplantation [4,5].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

et al. 2011

View full text Add to dashboard Cite

Background: Pancreatic islet transplantation is a promising option for the treatment of diabetic patients; xenotransplantation of porcine islet cells would be a possibility to overcome the shortage of donor organs. Usually the donor pancreas is preserved with University of Wisconsin (UW) solution. A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical and UW solution, is superior to simple cold storage. However, the extensive use of TLM is cost intensive and there is evidence that TLM only oxygenates small parts of the organ preserved. Another possibility to increase the oxygen supply during organ preservation would be the use of hyperbaric oxygenation (HBO) which increases the oxygen tension in fluids. The aim of this study was to evaluate the effect of pre-oxygenation of different preservation solutions on organ quality in terms of high energy phosphate levels as well as the occurrence of apoptosis and the induction of heat shock proteins and nitrosative stress induced cell death in porcine pancreatic tissue.Methods: Porcine pancreatic tissue was preserved in different preservation solutions with or without pre-oxygenation for 6 hours of cold ischemic time (CIT). Then, tissue specimens were harvested and high energy phosphate levels were determined by HPLC. Moreover, immunohistochemistry was performed in order to detect occurrence of apoptosis, heat shock protein 70 (HSP70) as well as nitrosative stress induced cell death.Results: Organs stored in pre-oxygenated UW solution showed best results in terms of high energy phosphate levels; apoptotic cells per islet as well as HSP70 positivity were significantly less when compared to simple UW storage and all other organ preservation solutions with or without pre-oxygenation.Conclusions: Pre-oxygenation of UW solution is a simple and promising method to improve islet cell quality after cold organ storage. However, further in vitro experiments have to be performed in order to confirm these findings.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Recent papers tested the feasibility of subcutaneous allogeneic islet transplantation in a large animal model (ie, minipig) within a novel oxygen-refueling and immunoprotective macrochamber [117], whereas other microcapsules were tested to also enable cell imaging [118] or to create a prevascularized microenvironment [119]. Despite major improvements in the biomaterials developed for islet encapsulation and for the incorporation of molecules enhancing islet function or enabling graft monitoring, some issues (ie, long-term oxygen diffusion and nutrient transport, biocompatibility and viability) need to be addressed.…”

Section: Is It Possible To Overcome Late Challenges Of Intraliver Islmentioning

confidence: 99%

Alternative Transplantation Sites for Pancreatic Islet Grafts

Cantarelli¹,

Piemonti²

2011

Curr Diab Rep

126

100

View full text Add to dashboard Cite

The liver is the current site of choice for pancreatic islet transplantation, even though it is far from being an ideal site because of immunologic, anatomic, and physiologic factors leading to a significant early graft loss. A huge amount of alternative sites have been used for islet transplantation in experimental animal models to provide improved engraftment and long-term survival minimizing surgical complications. The pancreas, gastric submucosa, genitourinary tract, muscle, omentum, bone marrow, kidney capsule, peritoneum, anterior eye chamber, testis, and thymus have been explored. Site-specific differences exist in term of islet engraftment, but few alternative sites have potential clinical translation and generally the evidence of a post-transplant islet function better than that reached after intraportal infusion is still lacking. This review discusses site-specific benefits and drawbacks taking into account immunologic, metabolic, and technical aspects to identify the ideal microenvironment for islet function and survival.

show abstract

“…Stiegler et al used a combination of foam dressing, vacuum-assisted wound closure, and hyperbaric oxygenation (HBO) in rats, with results indicating increased vessel ingrowth and vascular endothelial growth factor (VEGF) levels dependent on duration of HBO treatment. Perfusion was significantly improved in the experimental group with only a small amount of apoptosis following transplantation [233]. Similarly, islet cells transplanted subcutaneously with adipose tissue-derived stromal cells (ADSCs), and minced adipose tissue showed increased vascularization and higher capillary density than mice implanted with either ADSCs or minced adipose tissue alone [234].…”

Section: Ongoing Debate and Limitationsmentioning

confidence: 99%

Evolution of -Cell Replacement Therapy in Diabetes Mellitus: Islet Cell Transplantation

Jahansouz

Kumer

et al. 2011

Journal of Transplantation

View full text Add to dashboard Cite

Diabetes mellitus remains one of the leading causes of morbidity and mortality worldwide. According to the Centers for Disease Control and Prevention, approximately 23.6 million people in the United States are affected. Of these individuals, 5 to 10% have been diagnosed with Type 1 diabetes mellitus (T1DM), an autoimmune disease. Although it often appears in childhood, T1DM may manifest at any age, leading to significant morbidity and decreased quality of life. Since the 1960s, the surgical treatment for diabetes mellitus has evolved to become a viable alternative to insulin administration, beginning with pancreatic transplantation. While islet cell transplantation has emerged as another potential alternative, its role in the treatment of T1DM remains to be solidified as research continues to establish it as a truly viable alternative for achieving insulin independence. In this paper, the historical evolution, procurement, current status, benefits, risks, and ongoing research of islet cell transplantation are explored.

show abstract

Creation of a prevascularized site for cell transplantation in rats

Cited by 22 publications

References 77 publications

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

Alternative Transplantation Sites for Pancreatic Islet Grafts

Evolution of -Cell Replacement Therapy in Diabetes Mellitus: Islet Cell Transplantation

Contact Info

Product

Resources

About