Creation of borer pests resistance genetically engineering peach (Prunus persica L.) plants by constitutively overexpressing the cry1Ab gene

Kadasa, Naif M. S.; Metwali, E. M. R.; Soliman, Hemaid I. A.; Alshehri, Wafa A.

doi:10.1007/s11240-021-02198-w

Cited by 2 publications

(2 citation statements)

References 56 publications

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“…One other possibility would be to clone the TNL1 gene and transform a susceptible peach with a TNL1 construction. In vitro peach regeneration and genetic transfer with Agrobacterium is difficult, but the first transgenic peach plant carrying the cry1Ab gene for insect resistance was successfully produced recently [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

The Rm1 and Rm2 Resistance Genes to Green Peach Aphid (Myzus persicae) Encode the Same TNL Proteins in Peach (Prunus persica L.)

Duval

Heurtevin

Dlalah

et al. 2022

Genes

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The green peach aphid (GPA), Myzus persicae, is an important pest of the peach crop. Three major dominant resistance genes have already been detected, Rm1 in the Weeping Flower Peach (WFP) clone, Rm2 in the Rubira clone, and Rm3 in the Fen Shouxing clone. In this study, after NGS resequencing of WFP and Rubira, we found that their genomic sequences in the Rm1 and Rm2 region were similar but very different from that of the susceptible reference peach Lovell. We constructed a BAC library for the GPA-resistant WFP and screened four BAC clones to sequence the target region. The new sequence was 61.7 Kb longer than Lovell and was annotated with four different TIR_NBS_LRR genes. Among them, the TNL1 gene was very overexpressed in WFP leaves 24 h after GPA infestation. This gene was also present and expressed in the Rubira clone and had the same sequence as the candidate Rm3 gene, supporting the hypothesis that the three genes share the same origin. In addition, we identified a second TNL, TNL2, located at 35.4 Kb from TNL1 and slightly overexpressed after GPA infestation. Kasp and size molecular markers were designed for use in marker-assisted selection and were validated in a peach segregating population.

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Section: Discussionmentioning

confidence: 99%

The Rm1 and Rm2 Resistance Genes to Green Peach Aphid (Myzus persicae) Encode the Same TNL Proteins in Peach (Prunus persica L.)

Duval

Heurtevin

Dlalah

et al. 2022

Genes

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“…However, functional gene analysis in peach lags behind other species of its family because there are no in vitro regeneration and transformation methods for generating transgenic peach plants. To the best of our knowledge, the generation of transgenic peach has been reported in four published articles, but most of these methods have proven to be impossible to repeat [ 1 , 2 ]. Several methods of transient expression have been developed to analyse the functions of certain classes of genes, such as those related to volatile compound production, ABA biosynthesis, or anthocyanin production [ 3–6 ].…”

Section: Introductionmentioning

confidence: 99%

A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings

Cheng,

Shao,

et al. 2024

Horticulture Research

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Stable genetic transformation of peach [Prunus persica (L.) Batsch] still faces many technical challenges, and existing transient expression methods are limited by tissue type or developmental stage, making it difficult to conduct functional analysis of genes regulating shoot growth. To overcome this dilemma, we developed a three-step method for efficient analysis of gene functions during peach seedling growth and development. This method resulted in transformation frequencies ranging from 48 to 87%, depending on the gene. From transformation of germinating seeds to phenotyping of young saplings took just 1.5 months and can be carried out any time of year. To test the applicability of this method, the function of three tree architecture-related genes, namely PpPDS, PpMAX4 and PpWEEP, and two lateral root-related genes, PpIAA14-1 and -2, were confirmed. Since functional redundancy can challenge gene functional analyses, tests were undertaken with the growth-repressor DELLA, which has three homologous genes, PpDGYLA (DG), PpDELLA1 (D1) and -2 (D2), in peach that are functionally redundant. Silencing using a triple-target vector (TRV2-DG-D1-D2) resulted in transgenic plants taller than those carrying just TRV2-DG or TRV2. Simultaneously silencing the three DELLA genes also attenuated the stature of two dwarf genotypes, ‘FHSXT’ and ‘HSX’, which normally accumulate DELLA proteins. Our study provides a method for the functional analysis of genes in peach and can be used for the study of root, stem and leaf development. We believe this method can be replicated in other woody plants.

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Creation of borer pests resistance genetically engineering peach (Prunus persica L.) plants by constitutively overexpressing the cry1Ab gene

Cited by 2 publications

References 56 publications

The Rm1 and Rm2 Resistance Genes to Green Peach Aphid (Myzus persicae) Encode the Same TNL Proteins in Peach (Prunus persica L.)

The Rm1 and Rm2 Resistance Genes to Green Peach Aphid (Myzus persicae) Encode the Same TNL Proteins in Peach (Prunus persica L.)

A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings

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