Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

Cisneros, Arturo; Garcia, Reinerio Benega; Tel-Zur, Noemi

doi:10.1007/s10681-012-0800-3

Cited by 12 publications

(15 citation statements)

References 27 publications

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“…Among the 32 true hybrids obtained, all eight (25%) allohexaploids were obtained from embryo rescue at 10 DAP (pro-embryo stage), showing that the chromosome doubling event occurs very early in embryo development. We note here that in a previous report (Cisneros et al, 2013) two plants regenerated from embryo rescue 30 DAP following H. megalanthus × H. undatus crossing were reported in error as a diploid and a triploid, whereas they were, in fact, tetraploids. Tissues in Hylocereus species are rich in polysaccharides, making the flow cytometric analysis a challenging task.…”

Section: Discussionmentioning

confidence: 45%

“…undatus cross combination and the lack of allotriploids in both reciprocal crosses suggest that there are (still) unknown factor (s) preventing the formation and development of a triploid embryo. Hylocereus species bear large flowers (30-35 cm diameter) with numerous mega-and microspores, which simplify technical manipulations (Nerd and Mizrahi, 1997), such as hand cross pollination and the embryo rescue technique (Cisneros et al, 2013). Thus, from a technical point of view, Hylocereus species can be used as good model plants in polyploid studies.…”

Section: Discussionmentioning

confidence: 99%

“…ex Vaupel) Moran] Bauer (accession 90-003) as the female parent and the diploid H. undatus (Haw) Br. and Rose (accession 89-024) as the male parent, according to the embryo rescue technique given in Cisneros et al (2013) and described briefly below. It has previously been shown that the total number of seeds per fruit in H. megalanthus is about 200-300, resulting in 20-35% aborted/empty seeds (Cisneros et al, 2011;Tel-Zur et al, 2011).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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In Support of Winge's Theory of “Hybridization Followed by Chromosome Doubling”

et al. 2020

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Polyploidy-or chromosome doubling-plays a significant role in plant speciation and evolution. Much of the existing evidence indicates that fusion of unreduced (or 2n) gametes is the major pathway responsible for polyploid formation. In the early 1900s, a theory was put forward that the mechanism of "hybridization followed by chromosome doubling" would enable the survival and development of the hybrid zygote by providing each chromosome with a homolog with which to pair. However, to date there is only scant empirical evidence supporting this theory. In our previous study, interspecific-interploid crosses between the tetraploid Hylocereus megalanthus, as the female parent, and the diploid H. undatus, as the male parent, yielded only allopentaploids, allohexaploids, and 5x-and 6x-aneuploids instead of the expected allotriploids. No viable hybrids were obtained from the reciprocal cross. Since H. undatus underwent normal meiosis with regular pairing in the pollen mother cells and only reduced pollen grains were observed, the allohexaploids obtained supported the concept of "chromosome doubling." In this work, we report ploidy level, fruit morphology, and pollen viability and diameter in a group of putative hybrids obtained from an embryo rescue procedure following controlled H. megalanthus × H. undatus crosses, with the aim to elucidate, for the first time, the timing and developmental stage of the chromosome doubling. As in our previous report, no triploids were obtained, but tetraploids, pentaploids, hexaploids, and 5x-and 6xaneuploids were found in the regenerated plants. The tetraploids exhibited the morphological features of the maternal parent and could not be considered true hybrids. Based on our previous studies, we can assume that the pentaploids were a result of a fertilization event between one unreduced (2n) female gamete from the tetraploid H. megalanthus and a normal (n) haploid male gamete from H. undatus. All the allohexaploids obtained from the embryo rescue technique where those that regenerated from fertilized ovules 10 days after pollination (at the pro-embryo stage), showing that the chromosome doubling event occurred at a very early development stage, i.e., at the zygote stage or shortly after zygote formation. These allohexaploids thus constitute empirical evidence of "hybridization followed by chromosome doubling."

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Section: Discussionmentioning

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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In Support of Winge's Theory of “Hybridization Followed by Chromosome Doubling”

et al. 2020

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“…One of the most important factors for this technique to be successful is to remove the embryo at the early stages of development. Cisneros et al (2013) established an in vitro system for rescuing embryos from interspecific-interploid crosses between Hylocereus sp. (A. Berger) Britton & Rose using a tetraploid H. megalacanthus (Vaup.)…”

Section: Embryo Rescuementioning

confidence: 99%

Tissue culture of ornamental cacti

Pérez-Molphe-Balch

Santos-Díaz

Ramírez-Malagón

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family.

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“…Embryo rescue is to nurture the immature or weak embryo, thus allowing it the chance to survive, and plays an important role in modern plant breeding (Sharma et al, 1996). Embryo rescue also demonstrates potential roles in cassava breeding: (1) rescue plantlets from the younger fruits when confronted with adverse climate (Uma et al, 2011); (2) rescue in vitro plantlets following interspecific hybridization (Clarke et al, 2011;Cisneros et al, 2013); (3) shorten the breeding cycle by recovering the plantlets from younger seeds, replace germinating the seeds harvested after breaking the dormancy (Kagithoju et al, 2013;Gbadamosi, 2013). But initial work required considerable amount of basic research because of the scarcity of information related to the biology or structural development of flower buds in cassava (Perera et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Fruit, seed and embryo development of different cassava (Manihot esculenta Crantz) genotypes and embryo rescue

Yan¹,

Lu²,

Alzate³

et al. 2014

Afr. J. Biotechnol.

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Fruit, seed and embryo developments of different cassava (Manihot esculenta Crantz) genotypes, as well as embryo rescue, were investigated. The fruits of three genotypes after uncontrolled open pollination presented the same progressive development with similar sizes at different stages. There are large differences in the fruit set as well as the embryo development between different genotypes. Days after pollination (DAP) was found not to be an adequate predictor of embryo size as their size ranged from almost invisible to 8.7 mm in length at 32 DAP even within the different locules of the same fruit. The ideal stage for embryo rescue in cassava was from 32 to 36 DAP, because at that stage most embryos are visible (> 0.7 mm); and their excision without injury is feasible. Also, in vitro germination of the cotyledonary embryos at that stage had a high success rate. A half Murashige and Skoog (MS) medium supplemented with 1.0 mg/l GA 3 , 2% sucrose and 0.2% gel rite proved to be adequate for embryo rescue.

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Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

Cited by 12 publications

References 27 publications

In Support of Winge's Theory of “Hybridization Followed by Chromosome Doubling”

In Support of Winge's Theory of “Hybridization Followed by Chromosome Doubling”

Tissue culture of ornamental cacti

Fruit, seed and embryo development of different cassava (Manihot esculenta Crantz) genotypes and embryo rescue

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