CREB is involved in mouse annexin A1 regulation by cAMP and glucocorticoids

Abstract

A cAMP and some glucocorticoid response elements have been underlined in the promoter of mouse annexin A1. To analyse the function of these DNA sequences, the role of cAMP and glucocorticoids, as well as the transcription factors involved in their activation, were investigated. A construct containing 1381 bp of the DNA 5 H -flanking annexin A1 gene fused to LacZ was used. The level of activation of the reporter gene was analysed by transient transfection of the JEG3 cell line. Activation of b-galactosidase expression was observed with both dibutyryl cAMP and dexamethasone when compared with cells treated with serum only. Simultaneous addition of dexamethasone and dibutyryl cAMP did not result in a synergistic effect but rather in a competitive one. Gel-shift assays with a probe including the cAMP response element-like element of the annexin A1 promoter revealed a main specific DNA±protein complex when cells were stimulated with dibutyryl cAMP and/or dexamethasone. In all cases CREB protein was identified by supershift analysis. We therefore conclude that this cAMP response element sequence plays a prominent role in the transactivation of the annexin A1 promoter by dibutyryl cAMP and that it is involved in the response to glucocorticoids.

Dexamethasone‐induced and estradiol‐induced CREB activation and annexin 1 expression in CCRF‐CEM lymphoblastic cells: evidence for the involvement of cAMP and p38 MAPK

“…20,21 Therefore, some authors have suggested that the putative GRE that is present in the promoter region (intron I) of the ANXA1 gene is non-functional. 22,23 In our previous work, we also found no evidence supporting the involvement of such nuclear hormone receptors in either Dex-induced or E 2 b-induced ANXA1 synthesis in CCRF-CEM cells. 4,16 Besides the putative GRE, it was recently shown that the promoter region of the ANXA1 gene contains a cAMP-responsive element (CRE) and that a CREbinding protein (CREB) is required for either Dexinduced or cAMP-induced ANXA1 synthesis.…”

Dexamethasone represses cAMP rapid upregulation of TRH gene transcription: identification of a composite glucocorticoid response element and a cAMP response element in TRH promoter

“…The interference caused by dex (or GR) on 8Br-cAMP (or CREB) activation on proTRH mRNA levels also occurred at the transcriptional level as observed for CRH (Guardiola-Díaz et al 1996, Malkoski et al 1997, King et al 2002, vasopressin (Kuwara et al 2003) or the -subunit gene of glycoprotein hormone (Stauber et al 1992); while in some of these cases dex alone can cause inhibition or no effect, TRH regulation coincides with that of annexin A1 that is stimulated by dex or cAMP but when combined, inhibition is observed (Antonicelli et al 2001).…”

Annexin-1 Regulates Growth Arrest Induced by High Levels of Estrogen in MCF-7 Breast Cancer Cells

“…It was recently shown that the promoter region of the mouse Anx-A1 gene contains a CRE and that a CREB is required for cAMP-induced ANXA1 synthesis (36). 17h-E has also been shown to influence CRE-mediated gene transcription through rapid phosphorylation of CREB (37).…”