Effect of relative humidity (RH) and temperatures on formaldehyde emission (FE) from medium density fibreboard (MDF) subjected to load was investigated. For this purpose, flexural creep tests at 15 and 30% of ultimate bending loads were performed by using flexural creep equipment. The samples were exposed to loads at RH of 45, 65 and 85% and temperatures of 15°C, 23°C and 30°C for 72 hours. Subsequently, FEs of samples were measured by the flask method. The results indicated that FE in loaded samples is greater than unloaded samples. FE in all samples (unloaded and loaded) increased with increasing RH and temperature. The highest FE was observed in higher load level, higher RH and at higher temperature. Temperature was more effective than RH on FE from MDF.