Crenarchaeol

Damsté, Jaap S. Sinninghe; Schouten, Stefan; Hopmans, Ellen C.; Duin, Adri C. T. van; Geenevasen, Jan A. J.

doi:10.1194/jlr.m200148-jlr200

Cited by 581 publications

(114 citation statements)

References 30 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…This is the expected IPL-GDGT signature of Thaumarchaeota as previously observed in pure cultures (Elling et al, 2017;Pitcher et al, 2010;Schouten et al, 2008;Sinninghe Damsté et al, 2012). The predominance of the HPH IPL type in surface (0-2 cm) sediments was previously interpreted as an indication of the presence of an active Thaumarchaeotal population synthesizing membrane lipids in situ (Lengger et al, 2012(Lengger et al, , 2014, taking into account the labile nature of sedimentary phospholipids (Harvey et al, 1986;Schouten et al, 2010;Xie et al, 2013).…”

Section: Archaeal Community Composition In the Anoxic Subsurface Sedisupporting

confidence: 79%

“…GDGTs containing zero to four cyclopentane moieties (Fig. S1 in the Supplement) are usually not exclusive to a specific archaeal group (Schouten et al, 2013), with the exception of the GDGT crenarchaeol, containing four cyclopentane moieties and one cyclohexane moiety, which is deemed to be exclusive to the Thaumarchaeota phylum (De La Torre et al, 2008;Sinninghe Damsté et al, 2002, 2012. Recently, Lincoln et al (2014a) proposed Marine Group II as potential producers of crenarchaeol.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone

et al. 2018

Self Cite

View full text Add to dashboard Cite

Abstract. Benthic archaea comprise a significant part of the total prokaryotic biomass in marine sediments. Recent genomic surveys suggest they are largely involved in anaerobic processing of organic matter, but the distribution and abundance of these archaeal groups are still largely unknown. Archaeal membrane lipids composed of isoprenoid diethers or tetraethers (glycerol dibiphytanyl glycerol tetraether, GDGT) are often used as archaeal biomarkers. Here, we compare the archaeal diversity and intact polar lipid (IPL) composition in both surface (0-0.5 cm) and subsurface (10-12 cm) sediments recovered within, just below, and well below the oxygen minimum zone (OMZ) of the Arabian Sea. Archaeal 16S rRNA gene amplicon sequencing revealed a predominance of Thaumarchaeota (Marine Group I, MG-I) in oxygenated sediments. Quantification of archaeal 16S rRNA and ammonia monoxygenase (amoA) of Thaumarchaeota genes and their transcripts indicated the presence of an active in situ benthic population, which coincided with a high relative abundance of hexose phosphohexose crenarchaeol, a specific biomarker for living Thaumarchaeota. On the other hand, anoxic surface sediments within the OMZ and all subsurface sediments were dominated by archaea belonging to the Miscellaneous Crenarchaeota Group (MCG), the Thermoplasmatales and archaea of the DPANN (superphylum grouping Micrarchaeota, Diapherotrites, Aenigmarchaeota, Nanohaloarchaeota, Parvarchaeota, Nanoarchaeota, Pacearchaeota and Woesearchaeota). Members of the MCG were diverse, with a dominance of subgroup MCG-12 in anoxic surface sediments. This coincided with a high relative abundance of IPL GDGT-0 with an unknown polar head group. Subsurface anoxic sediments were characterized by higher relative abundance of GDGT-0, -2 and -3 with dihexose IPL types, GDGT-0 with a cyclopentanetetraol molecule and hexose, as well as the presence of specific MCG subgroups, suggesting that these groups could be the biological sources of these archaeal lipids.

show abstract

Section: Archaeal Community Composition In the Anoxic Subsurface Sedisupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although marine Crenarchaeota span the depth continuum (6), their numbers are greatest in waters just below the photic zone (3,7). Isotopic analyses of lipids suggest that marine Crenarchaeota have the capacity for autotrophic carbon assimilation (8)(9)(10)(11)(12). The recent isolation of Nitrosopumilus maritimus, the first cultivated nonthermophilic crenarchaeon, demonstrated conclusively that bicarbonate and ammonia can serve as sole carbon and energy sources for at least some members of this lineage (13).…”

mentioning

confidence: 99%

“…Although yet uncultivated, C. symbiosum can be harvested in significant quantities from host tissues, where it comprises up to 65% of the total microbial biomass (20,21). These enriched uniarchaeal preparations of C. symbiosum have facilitated DNA analyses (20,22,23), as well as the identification and structural elucidation of nonthermophilic crenarchaeotal core lipids (8,24,25).…”

mentioning

confidence: 99%

Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum

Hallam

Konstantinidis

Putnam

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

423

367

View full text Add to dashboard Cite

Crenarchaeota are ubiquitous and abundant microbial constituents of soils, sediments, lakes, and ocean waters. To further describe the cosmopolitan nonthermophilic Crenarchaeota, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a-and b-type ribosomal RNA variants. Although they were syntenic, overlapping a-and b-type ribotype genomes harbored significant variability. A single tiling path comprising the dominant a-type genotype was assembled and used to explore the genomic properties of C. symbiosum and its planktonic relatives. Of 2,066 ORFs, 55.6% matched genes with predicted function from previously sequenced genomes. The remaining genes partitioned between functional RNAs (2.4%) and hypotheticals (42%) with limited homology to known functional genes. The latter category included some genes likely involved in the archaeal-sponge symbiotic association. Conversely, 525 C. symbiosum ORFs were most highly similar to sequences from marine environmental genomic surveys, and they apparently represent orthologous genes from free-living planktonic Crenarchaeota. In total, the C. symbiosum genome was remarkably distinct from those of other known Archaea and shared many core metabolic features in common with its free-living planktonic relatives.

show abstract

“…One of the most ubiquitous isoGDGTs is crenarchaeol, a unique membrane lipid with a cyclohexane moiety. Crenarchaeol is produced by the marine archaea belonging to the phylum Thaumarchaeota (Sinninghe Damsté et al, 2002;Pitcher et al, 2011). Most species of Thaumarchaeota have been shown to be chemoautotrophs and are ammonia oxidizers (e.g., Könneke et al, 2005;Wuchter et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Data report: distribution and sources of tetraether lipids in Oligocene deposits from the western North Atlantic, IODP Sites U1406 and U1411

Śliwińska¹,

Mets²,

Schouten³

2017

Proceedings of the IODP

View full text Add to dashboard Cite

We have analyzed the distribution of glycerol dialkyl glycerol tetraether lipids (GDGTs) in the Oligocene succession recovered by Integrated Ocean Drilling Program Expedition 342. The two studied sites, U1406 and U1411, penetrate a highly expanded section of the upper Eocene-Oligocene-lower Miocene succession.The branched isoprenoid tetraether (BIT) index varies between 0.2 and 0.85, implying that there was a moderate to high influx of soil organic matter to both sites. Furthermore, during the late Oligocene (Chattian) the influx was lower than during the early Oligocene (Rupelian). The methane index (MI) and %GDGT-0 index values suggest that methanotrophic Euryarchaeota and methanogenic archaea are not the main source of GDGTs, but rather marine Thaumarchaeota.

show abstract

Crenarchaeol

Cited by 581 publications

References 30 publications

Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone

Benthic archaea as potential sources of tetraether membrane lipids in sediments across an oxygen minimum zone

Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum

Data report: distribution and sources of tetraether lipids in Oligocene deposits from the western North Atlantic, IODP Sites U1406 and U1411

Contact Info

Product

Resources

About