Zucchini culture is slightly sensitive to salinity and is among the ten vegetables of highest economic value, with characteristics of precocity and easy cultivation. These characteristics are some of the reasons for the expansion of its cultivation among small producers. Thus, the objective was to evaluate the effect of different levels of salinity in the irrigation water and biofertilizer types on the initial growth of the zucchini (Cucurbita pepo L.) culture. The experiment was carried out in the full sunlight in a randomized block design, in a 5x2 factorial arrangement. The treatments consisted of a combination of five types of biofertilizers (quail, sheep, mixed, bovine, and crab) and two salinity levels of the irrigation water (0.8 and 2.5 dS m-1), with five replicates. At 30 days after transplanting, the following variables were evaluated: electrical conductivity of the substrate, plant height, number of leaves, roots length, stem diameter, leaf area, chlorophyll content, dry mass of the aerial part, root dry mass, and total dry mass. Salt stress negatively interferes with the accumulation of zucchini plant biomass. Quail, bovine, and sheep biofertilizers are more efficient about plant height, number of leaves, and stem diameter. The sheep biofertilizer attenuates the salt stress for the dry mass of the aerial part, the root, and the total dry mass.