Crimean Congo hemorrhagic fever: a brief report regarding kidney involvement

Khazaei, Zaher; Darvishi, Isan; Amiri, Masoud; Sohrabivafa, Malihe; Kamran, Shiva

doi:10.15171/jrip.2018.31

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…CCHFV causes a fatal human infection with high mortality rate. Onset of disease coincides with sudden fever, myalgia, dizziness and bodyache leading to nausea, vomiting, diarrhoea, neural impairments, kidney and liver failure after 5th day of illness that is associated with higher mortality 5 , 6 . The disease also represents hemorrhagic symptoms characterized by petechial rash on mucosal surfaces, haematuria, epistaxis and gum bleeding 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

Development of double antibody sandwich ELISA as potential diagnostic tool for rapid detection of Crimean-Congo hemorrhagic fever virus

Shrivastava

Kumar

Yadav

et al. 2021

Sci Rep

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Crimean-Congo hemorrhagic fever (CCHF) virus, a highly pathogenic viral agent is responsible for severe fatal hemorrhagic infections in many parts of the world. The early diagnosis of CCHF infection is important for successful clinical management and epidemiological control. The nucleoprotein (NP) of CCHFV being highly conserved and immunogenic is used as early diagnostic marker. In this study, we report a rapid and sensitive double antibody based antigen capture ELISA to detect Crimean-Congo hemorrhagic fever virus (CCHFV). Highly specific polyclonal and monoclonal antibody against NP has been generated and used as capture and detector antibody respectively. The assay was able to detect viral nucleoprotein in different matrices including human serum, ticks and culture supernatant. The detection limit of the developed sandwich ELISA assay was 25 ng of purified antigen. Comparison with a real time RT-PCR revealed its detection limit to be 1000 genome equivalents of CCHFV. Further the assay was comparatively evaluated with a commercial kit employing gamma irradiated CCHFV, revealing a sensitivity and specificity of 100%. This newly developed sandwich ELISA (sELISA) with high sensitivity and specificity could be used as an efficient method for the detection of CCHF virus in humans, ticks and culture supernatant. The assay will be useful as alternate tool for diagnosis of acute infection and is amenable for screening of large scale samples in resource limited settings.

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Section: Introductionmentioning

confidence: 99%

Development of double antibody sandwich ELISA as potential diagnostic tool for rapid detection of Crimean-Congo hemorrhagic fever virus

Shrivastava

Kumar

Yadav

et al. 2021

Sci Rep

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“…A wide range of cells originating from invertebrates to vertebrates has so far been demonstrated to be susceptible to CCHFV [35][36][37]. Although it was previously shown that the liver is one of the primary replication sites of CCHFV [14,38,39], the virus has systematically spread to other tissues such as the kidney [20][21][22]. Since human and mammalian kidney cell lines such as human adrenal gland/cortex (SW-13), rhesus monkey kidney (LLC-MK2), or Syrian golden hamster kidney (BHK-21) are among the most commonly used cells for in vitro CCHFV research [16][17][18], in this study we used kidney cells as well.…”

Section: Discussionmentioning

confidence: 99%

“…In vivo, kidneys are also affected during CCHFV infections in humans, with evidence for direct virally-induced damage in acute renal failure [ 20 , 21 ]. Furthermore, renal failure is usually related to higher mortality rates in human infections [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Growth Characteristics of Crimean-Congo Hemorrhagic Fever Virus in Kidney Cells of Human and Bovine Origin

Földes

Farzani

Ergünay

et al. 2020

Viruses

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Crimean-Congo hemorrhagic fever virus (CCHFV) causes a lethal tick-borne zoonotic disease with severe clinical manifestation in humans but does not produce symptomatic disease in wild or domestic animals. The factors contributing to differential outcomes of infection between species are not yet understood. Since CCHFV is known to have tropism to kidney tissue and cattle play an important role as an amplifying host for CCHFV, in this study, we assessed in vitro cell susceptibility to CCHFV infection in immortalized and primary kidney and adrenal gland cell lines of human and bovine origin. Based on our indirect fluorescent focus assay (IFFA), we suggest a cell-to-cell CCHF viral spread process in bovine kidney cells but not in human cells. Over the course of seven days post-infection (dpi), infected bovine kidney cells are found in restricted islet-like areas. In contrast, three dpi infected human kidney or adrenal cells were noted in areas distant from one another yet progressed to up to 100% infection of the monolayer. Pronounced CCHFV replication, measured by quantitative real-time RT-PCR (qRT-PCR) of both intra- and extracellular viral RNA, was documented only in human kidney cells, supporting restrictive infection in cells of bovine origin. To further investigate the differences, lactate dehydrogenase activity and cytopathic effects were measured at different time points in all mentioned cells. In vitro assays indicated that CCHFV infection affects human and bovine kidney cells differently, where human cell lines seem to be markedly permissive. This is the initial reporting of CCHFV susceptibility and replication patterns in bovine cells and the first report to compare human and animal cell permissiveness in vitro. Further investigations will help to understand the impact of different cell types of various origins on the virus–host interaction.

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Transcriptome profiling highlights regulated biological processes and type III interferon antiviral responses upon Crimean-Congo hemorrhagic fever virus infection

Mo¹,

Feng²,

Dai³

et al. 2023

Virologica Sinica

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Crimean Congo hemorrhagic fever: a brief report regarding kidney involvement

Cited by 3 publications

References 11 publications

Development of double antibody sandwich ELISA as potential diagnostic tool for rapid detection of Crimean-Congo hemorrhagic fever virus

Development of double antibody sandwich ELISA as potential diagnostic tool for rapid detection of Crimean-Congo hemorrhagic fever virus

Differential Growth Characteristics of Crimean-Congo Hemorrhagic Fever Virus in Kidney Cells of Human and Bovine Origin

Transcriptome profiling highlights regulated biological processes and type III interferon antiviral responses upon Crimean-Congo hemorrhagic fever virus infection

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