Crimean–Congo Hemorrhagic Fever Virus Past Infections Are Associated with Two Innate Immune Response Candidate Genes in Dromedaries

Lado, Sara; Futas, Ján; Plášil, Martin; Loney, Tom; Weidinger, Pia; Camp, Jeremy V.; Kolodziejek, Jolanta; Kannan, D.; Hořín, Petr; Nowotny, Norbert; Burger, Pamela A.

doi:10.3390/cells11010008

Cited by 5 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Humans are the only known hosts that develop sickness following CCHFV infection, owing to the virus's asymptomatic presence in animal reservoirs, which allows it to circulate undetected [ 179 ]. Humans can become infected with CCHFV through tick bites or contact with contaminated animal tissues during and immediately after slaughter [ 180 , 181 ]. Notably, humans who had high CCHF seropositivity had a history of animal contact, animal husbandry, farming, and tick bites [ 182 ].…”

Section: Resultsmentioning

confidence: 99%

Global prevalence and species diversity of tick-borne pathogens in buffaloes worldwide: a systematic review and meta-analysis

et al. 2023

View full text Add to dashboard Cite

Background Buffaloes are important contributors to the livestock economy in many countries, particularly in Asia, and tick-borne pathogens (TBPs) commonly infect buffaloes, giving rise to serious pathologies other than their zoonotic potential. Methods The present investigation focuses on the prevalence of TBPs infecting buffaloes worldwide. All published global data on TBPs in buffaloes were collected from different databases (e.g., PubMed, Scopus, ScienceDirect, and Google Scholar) and subjected to various meta-analyses using OpenMeta[Analyst] software, and all analyses were conducted based on a 95% confidence interval. Results Over 100 articles discussing the prevalence and species diversity of TBPs in buffaloes were retrieved. Most of these reports focused on water buffaloes (Bubalus bubalis), whereas a few reports on TBPs in African buffaloes (Syncerus caffer) had been published. The pooled global prevalence of the apicomplexan parasites Babesia and Theileria, as well as the bacterial pathogens Anaplasma, Coxiella burnetii, Borrelia, Bartonella, and Ehrlichia in addition to Crimean-Congo hemorrhagic fever virus, were all evaluated based on the detection methods and 95% confidence intervals. Interestingly, no Rickettsia spp. were detected in buffaloes with scarce data. TBPs of buffaloes displayed a fairly high species diversity, which underlines the high infection risk to other animals, especially cattle. Babesia bovis, B. bigemina, B. orientalis, B. occultans and B. naoakii, Theileria annulata, T. orientalis complex (orientalis/sergenti/buffeli), T. parva, T. mutans, T. sinensis, T. velifera, T. lestoquardi-like, T. taurotragi, T. sp. (buffalo) and T. ovis, and Anaplasma marginale, A. centrale, A. platys, A. platys-like and “Candidatus Anaplasma boleense” were all were identified from naturally infected buffaloes. Conclusions Several important aspects were highlighted for the status of TBPs, which have serious economic implications for the buffalo as well as cattle industries, particularly in Asian and African countries, which should aid in the development and implementation of prevention and control methods for veterinary care practitioners, and animal owners. Graphical Abstract

show abstract

Section: Resultsmentioning

confidence: 99%

Global prevalence and species diversity of tick-borne pathogens in buffaloes worldwide: a systematic review and meta-analysis

et al. 2023

View full text Add to dashboard Cite

show abstract

“…We assumed there was a high possibility to find MERS-CoV or CCHFV in the sampled rodents and ticks because dromedary camels were kept nearby that were mainly used for tourism purposes. By concurrent sampling of these camels, we found CCHFV- as well as MERS-CoV-reactive antibodies in almost all of them [ 7 , 47 , 48 ]. CCHFV is a tick-borne virus, primarily maintained in ticks of the Hyalomma genus, and several mammalian livestock species act as amplifying hosts [ 7 , 8 , 9 ].…”

Section: Discussionmentioning

confidence: 99%

Potentially Zoonotic Viruses in Wild Rodents, United Arab Emirates, 2019—A Pilot Study

Weidinger

Kolodziejek

Khafaga³

et al. 2023

Viruses

Self Cite

View full text Add to dashboard Cite

The majority of emerging viral infectious diseases in humans originate from wildlife reservoirs, such as rodents and bats. We investigated a possible reservoir, namely wild gerbils and mice trapped in a desert reserve within the emirate of Dubai, United Arab Emirates (UAE). In total, 52 gerbils and 1 jird (Gerbillinae), 10 house mice (Mus musculus), and 1 Arabian spiny mouse (Acomys dimidiatus) were sampled. Oro-pharyngeal swabs, fecal samples, attached ticks, and organ samples (where available) were screened by (RT-q)PCR for the following viruses: Middle East respiratory syndrome-related coronavirus, Crimean-Congo hemorrhagic fever orthonairovirus, Alkhumra hemorrhagic fever virus, hantaviruses, Lymphocytic choriomeningitis mammarenavirus, Rustrela virus, poxviruses, flaviviruses, and herpesviruses. All of the samples were negative for all investigated viruses, except for herpesviruses: 19 gerbils (35.8%) and 7 house mice (70.0%) were positive. The resulting sequences were only partly identical to sequences in GenBank. Phylogenetic analysis revealed three novel betaherpesviruses and four novel gammaherpesviruses. Interestingly, species identification of the positive gerbils resulted in eight individuals clustering in a separate clade, most closely related to Dipodillus campestris, the North African gerbil, indicating either the expansion of the geographic range of this species, or the existence of a closely related, yet undiscovered species in the UAE. In conclusion, we could not find evidence of persistence or shedding of potentially zoonotic viruses in the investigated rodent cohorts of limited sample size.

show abstract

“…For example, Lado et al identified two candidate genes belonging to the innate immune system: FCAR and CLEC2B. The CLEC2B gene was associated with NK cell and stimulated natural killer cells to play an immune defense mechanism [ 27 ]. Mace and Orange demonstrated for the first time that CORO1A promoted NK cells to exert cytotoxic functions and immune secretion by regulating F-actin breakdown, thus exerting the function of lytic immune effectors [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and Validation of NK Marker Genes in Ovarian Cancer by scRNA-seq Combined with WGCNA Algorithm

Feng

2023

Mediators of Inflammation

View full text Add to dashboard Cite

Background. As an innate immune system effector, natural killer cells (NK cells) play a significant role in tumor immunotherapy response and clinical outcomes. Methods. In our investigation, we collected ovarian cancer samples from TCGA and GEO cohorts, and a total of 1793 samples were included. In addition, four high-grade serous ovarian cancer scRNA-seq data were included for screening NK cell marker genes. Weighted gene coexpression network analysis (WGCNA) identified core modules and central genes associated with NK cells. The “TIMER,” “CIBERSORT,” “MCPcounter,” “xCell,” and “EPIC” algorithms were performed to predict the infiltration characteristics of different immune cell types in each sample. The LASSO-COX algorithm was employed to build risk models to predict prognosis. Finally, drug sensitivity screening was performed. Results. We first scored the NK cell infiltration of each sample and found that the level of NK cell infiltration affected the clinical outcome of ovarian cancer patients. Therefore, we analyzed four high-grade serous ovarian cancer scRNA-seq data, screening NK cell marker genes at the single-cell level. The WGCNA algorithm screens NK cell marker genes based on bulk RNA transcriptome patterns. Finally, a total of 42 NK cell marker genes were included in our investigation. Among which, 14 NK cell marker genes were then used to develop a 14-gene prognostic model for the meta-GPL570 cohort, dividing patients into high-risk and low-risk subgroups. The predictive performance of this model has been well-verified in different external cohorts. Tumor immune microenvironment analysis showed that the high-risk score of the prognostic model was positively correlated with M2 macrophages, cancer-associated fibroblast, hematopoietic stem cell, stromal score, and negatively correlated with NK cell, cytotoxicity score, B cell, and T cell CD4+Th1. In addition, we found that bleomycin, cisplatin, docetaxel, doxorubicin, gemcitabine, and etoposide were more effective in the high-risk group, while paclitaxel had a better therapeutic effect on patients in the low-risk group. Conclusion. By utilizing NK cell marker genes in our investigation, we developed a new feature that is capable of predicting patients’ clinical outcomes and treatment strategies.

show abstract

Crimean–Congo Hemorrhagic Fever Virus Past Infections Are Associated with Two Innate Immune Response Candidate Genes in Dromedaries

Cited by 5 publications

References 48 publications

Global prevalence and species diversity of tick-borne pathogens in buffaloes worldwide: a systematic review and meta-analysis

Global prevalence and species diversity of tick-borne pathogens in buffaloes worldwide: a systematic review and meta-analysis

Potentially Zoonotic Viruses in Wild Rodents, United Arab Emirates, 2019—A Pilot Study

Identification and Validation of NK Marker Genes in Ovarian Cancer by scRNA-seq Combined with WGCNA Algorithm

Contact Info

Product

Resources

About