Criopreservação de eixos embrionários zigóticos de algodoeiro

Lopes, Kilson Pinheiro; Almeida, Francisco de Assis Cardoso; Carvalho, J. M. F. C.; Bruno, Riselane de Lucena Alcântara

doi:10.1590/s1415-43662013000300007

Cited by 12 publications

(8 citation statements)

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“…To ensure long-term quality of castor bean seeds, whether for marketing purposes or for preservation of genetic material, some techniques have been tested, among them storage of the seeds under sub-zero temperatures (Reed et al, 2011, Lopes et al, 2013. For better preservation of orthodox seeds, such as those of castor bean plants, environments with low relative humidity and temperature have proven to be appropriate, because such conditions, according to Chmielarz (2010), allow maintaining a low level of activity of the chemical reactions and preservation of the seeds germinative power and vigor.…”

Section: Introductionmentioning

confidence: 99%

Physiological and biochemical aspects of castor beans seeds deterioration stored in different packaging conditions and temperatures

Santos¹,

Carvalho²,

Caldeira³

et al. 2016

J. Seed Sci.

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-The present study aimed to evaluate effects of different storage conditions on the castor bean seed cultivar IAC-226 quality, stored for 12 months. For this purpose, seeds were stored in different environment and packaging conditions: in a cold chamber and conventional storage, using multiwall Kraft paper and in no vacuum and vacuum plastic packages at 1 atm; as well as under cryopreservation storage (-196 °C). Seed quality was evaluated before and after 4, 8 and 12 months of storage by germination tests, first count of germination, emergence percentage, emergence speed index and determination of changes in catalase (CAT) and superoxide dismutase (SOD) enzyme systems. Cryopreservation (-196 °C) is efficient in maintaining the physiological quality of castor bean cultivar IAC-226 for 12 months. The enzyme catalase stands out as a marker of castor seed deterioration during storage.Index terms: Ricinus communis L. storage, cryopreservation, vacuum.Aspectos bioquímicos e fisiológicos da deterioração de sementes de mamona armazenadas em diferentes embalagens e temperaturas RESUMO -Objetivou-se avaliar os efeitos de diferentes condições de armazenamento sobre a qualidade de sementes de mamona cultivar IAC-226, armazenadas por um período de 12 meses. Para isso, as sementes foram armazenadas em diferentes ambientes e tipos de embalagens: em câmara fria e armazém convencional, utilizando embalagens papel Kraft multifoliado e plástico com e sem acondicionamento a vácuo a 1 atm; e também armazenamento sob criopreservação (-196 °C). A qualidade das sementes foi avaliada antes e após 4, 8 e 12 meses de armazenamento pelos testes de geminação, primeira contagem de germinação, porcentagem de emergência, índice de velocidade de emergência e pela determinação das alterações nos sistemas enzimáticos catalase (CAT) e superóxido dismutase (SOD). A criopreservação (-196 °C) é eficiente na manutenção da qualidade fisiológica de sementes de mamona cultivar IAC-226 por 12 meses. A enzima catalase se destaca como um marcador da deterioração de sementes de mamona durante o armazenamento.Termos para indexação: Ricinus communis L., isoenzimas, criopreservação, armazenamento.1

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Section: Introductionmentioning

confidence: 99%

Physiological and biochemical aspects of castor beans seeds deterioration stored in different packaging conditions and temperatures

Santos¹,

Carvalho²,

Caldeira³

et al. 2016

J. Seed Sci.

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show abstract

“…It is therefore possible to preserve different plant materials for long periods, which by other methods would not be possible (BAJAJ, 1995;GALDIANO JUNIOR et al, 2012;LOPES et al, 2013). However, to prevent damage to the membranes of these materials due to the formation of ice crystals during the freezing process, cryoprotectant techniques can be used.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of seeds ofEncholirium spectabileMartius ex Schultes f. by the vitrification method

Ferrari

Colombo

Faria

et al. 2016

Revista Ciência Agronômica

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-The bromeliad Encholirium spectabile Martius ex Schultes f. is a endemic species of the Caatinga. The aim of this study was to evaluate the efficiencies of cryoprotectant in bromeliad seeds. The treatments consisted of immersing seeds in different cryoprotectant solutions and vitrification solutions before immersion in liquid nitrogen (NL) at -196 °C, as the following treatments: T1-Control: without cryoprotectants; T2-glycerol 2M (20 min) + PVS2 (10 min), T3-glycerol 2M (20 min) + PVS2 with phloroglucinol 1% (10 min), T4-sucrose 0.4M (20 min) + PVS2 (10 min); T5-sucrose 0.4M (20 min) + PVS2 with phloroglucinol 1% (10 min); T6-glycerol 2M (20 min) + sucrose 0.4M (20 min) + PVS2 (10 min), T7-glycerol 2M (20 min) + sucrose 0.4M (20 min) + PVS2 with phloroglucinol 1% (10 min). The experimental design was a completely randomized design with seven treatments and five replicates per treatment. Data were subjected to analysis of variance (ANOVA) and means were compared by the Tukey test at 5%. The seed moisture at the beginning of the experiment was 8.4%. There were statistically significant for the variables percentage of seeds germinated and seedling length, but for the variable seedling dry weight, T3 treatment was presented a value statistically high to T1, T4, T5 and T6, but did not differ from T2 and T7 treatments. It is concluded that for the cryopreservation of seeds of Encholirium spectabile is not necessary to use cryoprotectant solutions.Key words: Bromeliaceae. Conservation. Germination. Liquid nitrogen. RESUMO -A broméliaEncholirium spectabile Martius ex Schultes f. é uma espécie endêmica da Caatinga. O objetivo deste trabalho foi avaliar a eficiência de soluções crioprotetoras em sementes de bromélia. Os tratamentos consistiram da imersão das sementes em soluções crioprotetoras e de vitrificação, antes da imersão em nitrogênio líquido (NL) (-196 ºC), conforme os tratamentos a seguir: T1 -controle: sem crioprotetores; T2 -glicerol 2M (20 min) + PVS2 (10 min); T3 -glicerol 2M (20 min) + PVS2 com floroglucinol a 1% (10 min); T4 -sacarose 0,4M (20 min) + PVS2 (10 min); T5 -sacarose 0,4M (20 min) + PVS2 com floroglucinol a 1% (10 min); T6 -glicerol 2M (20 min) + sacarose 0,4M (20 min) + PVS2 (10 min); T7 -glicerol 2M (20 min) + sacarose 0,4M (20 min) + PVS2 com 1% de floroglucinol (10 min). O delineamento experimental foi o inteiramente casualizado com sete tratamentos e cinco repetições. Os dados foram submetidos à análise de variância (ANOVA) e as médias comparadas pelo teste de Tukey a 5%. O grau de umidade das sementes no início do experimento foi de 8,4%. Não apresentaram diferença estatística significativa para as variáveis porcentagem de sementes germinadas e comprimento de plântula, porém, para a variável massa seca de plântula, o tratamento T3 apresentou valor estatisticamente superior aos tratamentos T1, T4, T5 e T6, mas não diferiu dos demais tratamentos. Para a criopreservação de sementes da bromélia Encholirium spectabile não é necessário a utilização de soluções crioprotetoras.Palavras-...

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“…Genetic-dependent responses after cryopreservation have been observed for other species. Differences in the regeneration capacity and development of embryonic axes of Gossipium hirsutum were reported by Lopes et al (2013) [15]. Kaya & Souza (2017) [12] evaluated three Saccharum officinarum genotypes using different cryopreservation techniques of apical meristems observed variation in regeneration and growth.…”

Section: Discussionmentioning

confidence: 99%