CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip

Peyravian, Noshad; Kebria, Maziar Malekzadeh; Kiani, Jafar; Milan, Peiman Brouki; Mozafari, Masoud

doi:10.3390/ma14123164

Cited by 14 publications

(6 citation statements)

References 122 publications

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“…The bioprinting technology suitable for lung cancer research under a controlled microenvironment in a 3D tumor model, while therapeutical in anticancer drug screens in a breast ductal carcinoma and blood–brain barrier (BBB)-on-chip systems, is studied for a better understanding of disease progression . CRISPR-Associated microfluidic channels and chips provide services in a cost-effective and high-throughput manner . Digital technology such as deep learning for image digitization, data analysis, and automation contribute in OoC extensively .…”

Section: Trending Research Focused Technology and Limitationsmentioning

confidence: 99%

Advances in Organ-on-a-Chip Materials and Devices

Nahak¹,

Mishra²,

Preetam³

et al. 2022

ACS Appl. Bio Mater.

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The organ-on-a-chip (OoC) paves a way for biomedical applications ranging from preclinical to clinical translational precision. The current trends in the in vitro modeling is to reduce the complexity of human organ anatomy to the fundamental cellular microanatomy as an alternative of recreating the entire cell milieu that allows systematic analysis of medicinal absorption of compounds, metabolism, and mechanistic investigation. The OoC devices accurately represent human physiology in vitro; however, it is vital to choose the correct chip materials. The potential chip materials include inorganic, elastomeric, thermoplastic, natural, and hybrid materials. Despite the fact that polydimethylsiloxane is the most commonly utilized polymer for OoC and microphysiological systems, substitute materials have been continuously developed for its advanced applications. The evaluation of human physiological status can help to demonstrate using noninvasive OoC materials in real-time procedures. Therefore, this Review examines the materials used for fabricating OoC devices, the application-oriented pros and cons, possessions for device fabrication and biocompatibility, as well as their potential for downstream biochemical surface alteration and commercialization. The convergence of emerging approaches, such as advanced materials, artificial intelligence, machine learning, three-dimensional (3D) bioprinting, and genomics, have the potential to perform OoC technology at next generation. Thus, OoC technologies provide easy and precise methodologies in cost-effective clinical monitoring and treatment using standardized protocols, at even personalized levels. Because of the inherent utilization of the integrated materials, employing the OoC with biomedical approaches will be a promising methodology in the healthcare industry.

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Section: Trending Research Focused Technology and Limitationsmentioning

confidence: 99%

Advances in Organ-on-a-Chip Materials and Devices

Nahak¹,

Mishra²,

Preetam³

et al. 2022

ACS Appl. Bio Mater.

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“…COVID-19 has a strong association with advancing years. Men over the age of 75 who have a history of diabetes, hypertension, heart disease, chronic respiratory disease, cancer, or a history of surgery have the highest mortality rate [106][107][108]. The cellular immune system must be used to combat COVID-19 virus [109,110].…”

Section: Severe Covid-19 and Msc-based Therapy Treatment Optionsmentioning

confidence: 99%

Stem cell therapy for COVID-19 pneumonia

et al. 2022

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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus is a highly contagious microorganism, and despite substantial investigation, no progress has been achieved in treating post-COVID complications. However, the virus has made various mutations and has spread around the world. Researchers have tried different treatments to reduce the side effects of the COVID-19 symptoms. One of the most common and effective treatments now used is steroid therapy to reduce the complications of this disease. Long-term steroid therapy for chronic inflammation following COVID-19 is harmful and increases the risk of secondary infection, and effective treatment remains challenging owing to fibrosis and severe inflammation and infection. Sometimes our immune system can severely damage ourselves in disease. In the past, many researchers have conducted various studies on the immunomodulatory properties of stem cells. This property of stem cells led them to modulate the immune system of autoimmune diseases like diabetes, multiple sclerosis, and Parkinson's. Because of their immunomodulatory properties, stem cell-based therapy employing mesenchymal or hematopoietic stem cells may be a viable alternative treatment option in some patients. By priming the immune system and providing cytokines, chemokines, and growth factors, stem cells can be employed to build a long-term regenerative and protective response. This review addresses the latest trends and rapid progress in stem cell treatment for Acute Respiratory Distress Syndrome (ARDS) following COVID-19.

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“…Having said that, the efficiency of the delivery still remains a challenge in hematological-related malignancies. There has been special technique developed to shuttle the cargo of the CRISPR system into the cell via rapid cell mechanical deformation (Peyravian et al, 2021). This has, to a certain extent, alleviated the limitation posed earlier and has allowed high delivery efficiency in hematological malignancies.…”

Section: Summary and Future Perspectivesmentioning

confidence: 99%

Utilization of CRISPR-Mediated Tools for Studying Functional Genomics in Hematological Malignancies: An Overview on the Current Perspectives, Challenges, and Clinical Implications

et al. 2022

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Hematological malignancies (HM) are a group of neoplastic diseases that are usually heterogenous in nature due to the complex underlying genetic aberrations in which collaborating mutations enable cells to evade checkpoints that normally safeguard it against DNA damage and other disruptions of healthy cell growth. Research regarding chromosomal structural rearrangements and alterations, gene mutations, and functionality are currently being carried out to understand the genomics of these abnormalities. It is also becoming more evident that cross talk between the functional changes in transcription and proteins gives the characteristics of the disease although specific mutations may induce unique phenotypes. Functional genomics is vital in this aspect as it measures the complete genetic change in cancerous cells and seeks to integrate the dynamic changes in these networks to elucidate various cancer phenotypes. The advent of CRISPR technology has indeed provided a superfluity of benefits to mankind, as this versatile technology enables DNA editing in the genome. The CRISPR-Cas9 system is a precise genome editing tool, and it has revolutionized methodologies in the field of hematology. Currently, there are various CRISPR systems that are used to perform robust site-specific gene editing to study HM. Furthermore, experimental approaches that are based on CRISPR technology have created promising tools for developing effective hematological therapeutics. Therefore, this review will focus on diverse applications of CRISPR-based gene-editing tools in HM and its potential future trajectory. Collectively, this review will demonstrate the key roles of different CRISPR systems that are being used in HM, and the literature will be a representation of a critical step toward further understanding the biology of HM and the development of potential therapeutic approaches.

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CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip

Cited by 14 publications

References 122 publications

Advances in Organ-on-a-Chip Materials and Devices

Advances in Organ-on-a-Chip Materials and Devices

Stem cell therapy for COVID-19 pneumonia

Utilization of CRISPR-Mediated Tools for Studying Functional Genomics in Hematological Malignancies: An Overview on the Current Perspectives, Challenges, and Clinical Implications

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