CRISPR/Cas: History and Perspectives

Шмакова, А. А.; Shmakova, O. P.; Karpukhina, Anna; Vassetzky, Yegor S.

doi:10.1134/s1062360422040075

Cited by 8 publications

(4 citation statements)

References 73 publications

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“…The advent of engineered nucleases provided a new opportunity to precisely study translocations by introducing DSBs in the regions of interest. Specific chromosomal translocations can now be generated from precisely induced DSBs using programmed nucleases such as zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR) [ 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ]. A more sophisticated way to induce chromosomal translocations in cells would be to use proteins that naturally induce DNA damage and are known to be implicated in chromosomal translocations, reviewed in [ 85 , 86 ].…”

Section: Sources Of Dsbs In Vivo and Their Experimental Modeling For ...mentioning

confidence: 99%

Factors That Affect the Formation of Chromosomal Translocations in Cells

Canoy

Шмакова

Karpukhina

et al. 2022

Cancers

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Chromosomal translocations are products of the illegitimate repair of DNA double-strand breaks (DSBs). Their formation can bring about significant structural and molecular changes in the cell that can be physiologically and pathologically relevant. The induced changes may lead to serious and life-threatening diseases such as cancer. As a growing body of evidence suggests, the formation of chromosomal translocation is not only affected by the mere close spatial proximity of gene loci as potential translocation partners. Several factors may affect formation of chromosomal translocations, including chromatin motion to the potential sources of DSBs in the cell. While these can be apparently random events, certain chromosomal translocations appear to be cell-type-specific. In this review, we discuss how chromosomal translocations are formed and explore how different cellular factors contribute to their formation.

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Section: Sources Of Dsbs In Vivo and Their Experimental Modeling For ...mentioning

confidence: 99%

Factors That Affect the Formation of Chromosomal Translocations in Cells

Canoy

Шмакова

Karpukhina

et al. 2022

Cancers

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“…(23) The essay discussed the major turning points in the history of CC/Cas GE suppression equipment, from its original discovery to the present-day developments, including potential medicinal uses. (24) The research determines how well-known novel technologies including extreme pressure manufacturing, vibrated electricity, ultrasounds, oxygen manufacturing, ultraviolet radiation, a magnetic field, radiation from microwaves, non-thermal the bloodstream electrolyzed deteriorating water, and plasma therapy activated water affect the development and physical traits of various species of seeds. The causes of these technologies' beneficial and detrimental effects on germination rate have previously been examined.…”

Section: Introductionmentioning

confidence: 99%

Emerging Technologies in Industrial Microbiology: From Bioengineering to CRISPR-Cas Systems

Farooq,

Hanumanthayya,

Haq

2023

Salud, Ciencia y Tecnología

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Los avances de la biología sintética han propiciado la aparición de la "biología de ingeniería" mediante la equivalencia y la plataforma basada en un sistema biológico jerárquico, ortogonal y modularizado. Utilizando biopartes encontradas en bases de datos de secuencias, la ingeniería genómica (GE) es necesaria para crear células sintéticas con la funcionalidad adecuada. El sistema CRISPR, una de las diversas herramientas, está formado modularmente por la nucleasa Cas y el ARN guía, lo que simplifica la modificación de la GE a voluntad. Para modificar correctamente la GE a nivel de nucleótidos distintos, se han ideado recientemente muchas técnicas. El sistema CRISPR-Cas (CC) también se ha extendido al diagnóstico molecular de ácidos nucleicos y a la identificación de patógenos y virus causantes de enfermedades. Además, la biotecnología metabólica se está expandiendo para encontrar el objetivo de la metodología CC, que puede regular exactamente la producción de determinados genes en las células. En el presente artículo, evaluamos el estado actual de varias tecnologías de CC que pueden utilizarse en biología sintética y hablamos de cómo han evolucionado la biología artificial y la tecnología de CC en el campo de la microbiología.

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“…Clustered regularly interspaced short palindromic repeats-CRISPR associated protein (CRISPR-Cas) is an adaptive immune system present in some bacteria or archaea, and it has been developed as a genome-editing tool in a wide range of organisms including bacteria and eukaryotes [9]. The CRISPR-Cas system has been widely used for genome editing due to its convenience, simplicity and flexibility [10].…”

Section: Introductionmentioning

confidence: 99%

An Introduced RNA-Only Approach for Plasmid Curing via the CRISPR-Cpf1 System in Saccharomyces cerevisiae

Chen,

Lin

2023

Biomolecules

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The CRISPR-Cas system has been widely used for genome editing due to its convenience, simplicity and flexibility. Using a plasmid-carrying Cas protein and crRNA or sgRNA expression cassettes is an efficient strategy in the CRISPR-Cas genome editing system. However, the plasmid remains in the cells after genome editing. Development of general plasmid-curing strategies is necessary. Based on our previous CRISPR-Cpf1 genome-editing system in Saccharomyces cerevisiae, the crRNA, designed for the replication origin of the CRISPR-Cpf1 plasmid, and the ssDNA, as a template for homologous recombination, were introduced for plasmid curing. The efficiency of the plasmid curing was 96 ± 4%. In addition, we further simplified the plasmid curing system by transforming only one crRNA into S. cerevisiae, and the curing efficiency was about 70%. In summary, we have developed a CRISPR-mediated plasmid-curing system. The RNA-only plasmid curing system is fast and easy. This plasmid curing strategy can be applied in broad hosts by designing crRNA specific for the replication origin of the plasmid. The plasmid curing system via CRISPR-Cas editing technology can be applied to produce traceless products without foreign genes and to perform iterative processes in multiple rounds of genome editing.

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CRISPR/Cas: History and Perspectives

Cited by 8 publications

References 73 publications

Factors That Affect the Formation of Chromosomal Translocations in Cells

Factors That Affect the Formation of Chromosomal Translocations in Cells

Emerging Technologies in Industrial Microbiology: From Bioengineering to CRISPR-Cas Systems

An Introduced RNA-Only Approach for Plasmid Curing via the CRISPR-Cpf1 System in Saccharomyces cerevisiae

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