CRISPR/Cas-Mediated Abiotic Stress Tolerance in Crops

Ahmad, Aftab; Ashraf, Sidra; Munawar, Nayla; Jamil, Amer; Ghaffar, Abdul; Shahbaz, Muhammad

doi:10.1007/978-981-15-7142-8_6

Cited by 7 publications

(4 citation statements)

References 161 publications

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“…The CRISPR-based epigenome editing helps in protecting the plants from the harmful effects of salt stress [82]. The CRISPR/Cas9 protein has been used as a dCas9 is attached to the modifier of epigenetics for the alteration of gene expression [83].…”

Section: Salt Stress and Genome Engineeringmentioning

confidence: 99%

Salt Stress and Plant Molecular Responses

Chaudhry¹,

Gökçe²,

Gökçe³

2022

Plant Defense Mechanisms

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Plants being sessile suffer from several abiotic stresses during growth. These include drought, salinity, heavy metal, temperature, and nutrient stress. Salt stress is one of the devastating abiotic stresses that plant suffers under natural growth conditions. It is more common in arid regions due to excessive evaporation, which causes the accumulation of inorganic salts disrupting the plant metabolism. It also triggers the influence of drought stress, as plants are unable to absorb water. Additionally, it also causes oxidative stress in plant tissues. Thereby, plant adaptation to salt stress, rely on signals and pathways that help plant in establishing cellular ionic and osmotic homeostasis. Stress-responsive transcription factors play crucial roles in the regulation of gene expression in responses to salt stress. Moreover, genome editing has gained much attention for the engineering of traits for the better adaptation of plants to salt stress. This chapter elucidated the plant\'s physio-biochemical responses and molecular mechanisms to salt stress.

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Section: Salt Stress and Genome Engineeringmentioning

confidence: 99%

Salt Stress and Plant Molecular Responses

Chaudhry¹,

Gökçe²,

Gökçe³

2022

Plant Defense Mechanisms

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“…Sanghera et al, 2011;Song et al, 2016). CRISPR/Cas9 is developed as the most influential tool and can potentially develop crop tolerance to abiotic stresses (Ahmad et al, 2021). Genetic engineering and CRISPR/Cas9 use are not widely reported and demand further research.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Deciphering the myth of cold tolerance in soybean: An overview of molecular breeding applications

JIAN,

QIANG,

Guan YIJUN

et al. 2023

Not Bot Horti Agrobo

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The soybean is a source of several dietary components, including milk, protein, and oil. Cold stress has significantly curtailed soybean growth and yield in large areas and caused a high risk to global food security. The main objective of soybean breeders is to improve soybean resistance to cold stress. Conventional breeding approaches have made significant progress in developing cold tolerance in soybean; however, the high cost and complex genetic mechanism of cold tolerance hindered the large scale of these techniques. Molecular tools like quantitative trait loci (QTL), genome-wide association studies (GWAS), transcription factors (TFs), genetic engineering, and transcriptome have been used to identify cold tolerant genes/QTL and to develop soybean cultivars tolerant to cold stress. Clustered, regularly interspaced short palindromic repeats (CRISPR/Cas9) is used to increase the abiotic stress tolerance in soybean; however, its use to edit the cold tolerance genes in soybean is limited. Mapping of QTL has accelerated the master-assisted selection (MAS) in soybean. This review presents a detailed overview of molecular techniques and their use in developing cold-tolerant soybean cultivars. Using CRISPR/Cas9 would increase the speed of molecular breeding for cold tolerance in soybean. This information will assist soybean researchers in uncovering the basis of cold stress tolerance in soybean and adopting the most suitable way to breed the cold tolerant cultivars which can thrive under the extreme pressure of cold stress.

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Section: Introductionmentioning

confidence: 99%

“…In plant biotechnology, CRISPR/Cas systems are now routinely used for basic as well as applied research [ 40 , 41 ]. These systems have the potential to fulfill the early promises of genetic engineering, such as plants with higher yield, stronger biotic and abiotic resistance, and better nutritional value [ 29 , 41 ]. CRISPR crops are emerging on world markets, where they provide opportunities to boost food security, but the world is divided over their regulatory oversight.…”

Section: Introductionmentioning

confidence: 99%

An Outlook on Global Regulatory Landscape for Genome-Edited Crops

Ahmad

Munawar

Khan

et al. 2021

IJMS

Self Cite

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The revolutionary technology of CRISPR/Cas systems and their extraordinary potential to address fundamental questions in every field of biological sciences has led to their developers being awarded the 2020 Nobel Prize for Chemistry. In agriculture, CRISPR/Cas systems have accelerated the development of new crop varieties with improved traits—without the need for transgenes. However, the future of this technology depends on a clear and truly global regulatory framework being developed for these crops. Some CRISPR-edited crops are already on the market, and yet countries and regions are still divided over their legal status. CRISPR editing does not require transgenes, making CRISPR crops more socially acceptable than genetically modified crops, but there is vigorous debate over how to regulate these crops and what precautionary measures are required before they appear on the market. This article reviews intended outcomes and risks arising from the site-directed nuclease CRISPR systems used to improve agricultural crop plant genomes. It examines how various CRISPR system components, and potential concerns associated with CRISPR/Cas, may trigger regulatory oversight of CRISPR-edited crops. The article highlights differences and similarities between GMOs and CRISPR-edited crops, and discusses social and ethical concerns. It outlines the regulatory framework for GMO crops, which many countries also apply to CRISPR-edited crops, and the global regulatory landscape for CRISPR-edited crops. The article concludes with future prospects for CRISPR-edited crops and their products.

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CRISPR/Cas-Mediated Abiotic Stress Tolerance in Crops

Cited by 7 publications

References 161 publications

Salt Stress and Plant Molecular Responses

Salt Stress and Plant Molecular Responses

Deciphering the myth of cold tolerance in soybean: An overview of molecular breeding applications

An Outlook on Global Regulatory Landscape for Genome-Edited Crops

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