CRISPR-Cas systems mediated biosensing and applications in food safety detection

Liu, Jianghua; Wu, Di; Chen, Jiahui; Jia, Shaofeng; Wu, Yongning; Li, Guoliang

doi:10.1080/10408398.2022.2128300

Cited by 14 publications

(4 citation statements)

References 213 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…106 The continuous cycle of polymerization, nicking, and displacement of SDA can lead to exponential amplification and produce approximately 10 7 target fragments within 2 h at 37 1C. 70,[107][108][109] The combination of SDA with aptamer-based aptasensors has been applied in mycotoxin detection.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Recent advances in nucleic acid signal amplification-based aptasensors for sensing mycotoxins

Zhang,

Luo,

Cai

et al. 2024

Chem. Commun.

View full text Add to dashboard Cite

show abstract

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Recent advances in nucleic acid signal amplification-based aptasensors for sensing mycotoxins

Zhang,

Luo,

Cai

et al. 2024

Chem. Commun.

View full text Add to dashboard Cite

show abstract

“…To ensure food safety and monitor the proper use of antibiotics, there is a pressing need to develop efficient, sensitive, and rapid detection technologies to identify antibiotics. However, the current analytical methods have certain drawbacks, such as the requirement for centralized laboratories, precision analytical instruments, and time-consuming and complex procedures and the generation of toxic waste. ,, To overcome these challenges, adaptors for identifying specific antibiotics, such as chloramphenicol (CAP), oxytetracycline (OTC), and kanamycin (Kana), have been developed. Moreover, advanced biosensor technology and portable detection equipment based on the CRISPR/Cas system have been successfully employed for the detection of antibiotic residues …”

Section: Detection Of Non-nucleic Acid Targetsmentioning

confidence: 99%

CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

Li,

Zhao,

Liu

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic acid targets can enable us to monitor food contamination in a timely manner. In recent years, the CRISPR/Cas system has been extensively explored in biosensing. However, there is a lack of a summary of CRISPR/Cas-powered detection tailored to non-nucleic acid targets involved in food safety. This review comprehensively summarizes the recent advances on the construction of CRISPR/Cas-powered detection and the promising applications in the field of food safety related non-nucleic acid targets. The current challenges and futuristic perspectives are also proposed accordingly. The rapidly evolving CRISPR/Cas system has provided a powerful propellant for nonnucleic acid target detection via integration with aptamer and/or DNAzyme. Compared with traditional analytical methods, CRISPR/Cas-powered detection is conceptually novel, essentially eliminates the dependence on large instruments, and also demonstrates the capability for rapid, accurate, sensitive, and on-site testing.

show abstract

“…The technology can also be used to enhance the quality and flavor of food products, leading to better-tasting and longerlasting foods. Finally, by reducing the need for chemical pesticides and fertilizers, and by creating crops that are more resilient to environmental stressors, CRISPR-edited crops can contribute to more sustainable and environmentally friendly agricultural practices [3].…”

Section: Introductionmentioning

confidence: 99%

Application of CRISPR-Cas technology in food safety

Chen

2024

TNS

View full text Add to dashboard Cite

Food safety has become one of the most important public health issues in the world. Safety, nutrition, and food security are all interdependent. Unsafe food contributes to a cycle of illness and starvation that disproportionately affects young children, the elderly, and the ill. Therefore, it is essential to develop tests that are quick, efficient, and reliable. The CRISPR/Cas system is a bacterial acquired immune system that attacks invading DNA, plasmids, and phages. Genome editing using CRISPR/Cas offers new opportunities for plant breeding. Compared to animal cells, plant cells have rigid cell walls, making it challenging to deliver genome editing tools into plant cells. When using plants for industrial purposes, transgene insertion into the genome should be avoided. Therefore, delivery of Cas-gRNA ribonucleoprotein (RNP) into plant cells is preferred. This review proposed a novel RNP delivery strategy in rice and introduced a technology: whisker technology (commonly used for plant DNA delivery) to deliver RNPs into rice. This review also discussed ultrasound-assisted whisker technology via RNP management, combined with marker gene delivery, to identify genome editing events in rice decay cells in the absence of any other events, albeit at a lower frequency. Therefore, utilizing whiskers to generate RNP-based genome editing lineages in plants may be an attractive strategy.

show abstract

CRISPR-Cas systems mediated biosensing and applications in food safety detection

Cited by 14 publications

References 213 publications

Recent advances in nucleic acid signal amplification-based aptasensors for sensing mycotoxins

Recent advances in nucleic acid signal amplification-based aptasensors for sensing mycotoxins

CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

Application of CRISPR-Cas technology in food safety

Contact Info

Product

Resources

About