CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor

Zeng, Ruijin; Wang, Weijun; Chen, Mingming; Wan, Qing; Wang, Caicheng; Knopp, Dietmar; Tang, Dianping

doi:10.1016/j.nanoen.2020.105711

Cited by 314 publications

(169 citation statements)

References 33 publications

Supporting

Mentioning

167

Contrasting

Order By: Relevance

“…Notably, researchers have recently started to use CRISPR for in vitro detection of nucleic acids, and thus it is expected to be developed as a powerful and accurate molecular diagnostic tool [8] . With the single-stranded DNA Trans-cleavage activity of the Cas12a enzyme, combined with various isothermal nucleic acid amplification techniques, a series of CRISPR-Cas molecular diagnostic platforms based on fluorescent signal output have been developed [9] . Among the CRISPR-Cas effector family, Cas12 is an RNA-directed DNase that belongs to the class II V-A system, which induces the division of arbitrary single-stranded DNA (ssDNA) upon target recognition, which leads to the degradation of the ssDNA reporter and the release of a fluorescent signal at the division site, which can be detected by a portable method [10] , [11] , [12] .…”

Section: Introductionmentioning

confidence: 99%

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

Zhang¹,

Fan²,

Ding³

et al. 2022

Chemical Engineering Journal

View full text Add to dashboard Cite

In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system has good stability and reproducibility, and we expect that this method may provide a potential platform for the diagnosis of COVID-19.

show abstract

Section: Introductionmentioning

confidence: 99%

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

Zhang¹,

Fan²,

Ding³

et al. 2022

Chemical Engineering Journal

View full text Add to dashboard Cite

show abstract

“…Her research area includes spectral analysis and biosensors. tunable and abundant surface functionalities, ease of synthesizing large quantities in water, environmentfriendly characteristics as well as non-toxic nature, endow them brilliant application prospects in analytical chemistry [17], such as fluorometric sensing [18], piezoresistive sensing [19], and photothermal sensing [20], especially in electrochemical sensing.…”

Section: Introductionmentioning

confidence: 99%

Application of MXene in Electrochemical Sensors: A Review

Sun

et al. 2021

Electroanalysis

128

View full text Add to dashboard Cite

Electroanalysis has obtained considerable progress over the past few years, especially in the field of electrochemical sensors. Broadly speaking, electrochemical sensors include not only conventional electrochemical biosensors or non‐biosensors, but also emerging electrochemiluminescence (ECL) sensors and photoelectrochemical (PEC) sensors which are both combined with optical methods. In addition, various electrochemical sensing devices have been developed for practical purposes, such as multiplexed simultaneous detection of disease‐related biomarkers and non‐invasive body fluid monitoring. For the further performance improvement of electrochemical sensors, material is crucial. Recent years, a kind of two‐dimensional (2D) nanomaterial MXene containing transition metal carbides, nitrides and carbonitrides, with unique structural, mechanical, electronic, optical, and thermal properties, have attracted a lot of attention form analytical chemists, and widely applied in electrochemical sensors. Here, we reviewed electrochemical sensors based on MXene from Nov. 2014 (when the first work about electrochemical sensor based on MXene published) to Mar. 2021, dividing them into different types as electrochemical biosensors, electrochemical non‐biosensors, electrochemiluminescence sensors, photoelectrochemical sensors and flexible sensors. We believe this review will be of help to those who want to design or develop electrochemical sensors based on MXene, hoping new inspirations could be sparked.

show abstract

“…The synthesis of novel advanced materials can be important for the development of analytical methods for biomarker detection. Scaffold-based materials can be utilized in these advanced systems due to their superior physicochemical properties and relatively easy surface functionalization [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, there is also an obvious increasing trend in the use of scaffold-based materials in biosensing. Scaffolds or nano-scaffold-based materials have mostly been employed for the detection and quantification of analytes, such as cancer biomarkers [ 3 , 20 ], glucose [ [21] , [22] , [23] ], pharmaceutical compounds [ [24] , [25] , [26] ], hydrogen peroxide [ 27 ], neurotransmitters [ [28] , [29] , [30] ], amino acids [ 31 , 32 ], or inorganic ions [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Applications of scaffold-based advanced materials in biomedical sensing

Sarkhosh-Inanlou

Shafiei‐Irannejad

Azizi

et al. 2021

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

There have been many efforts to synthesize advanced materials that are capable of real-time specific recognition of a molecular target, and allow the quantification of a variety of biomolecules. Scaffold materials have a porous structure, with a high surface area and their intrinsic nanocavities can accommodate cells and macromolecules. The three-dimensional structure (3D) of scaffolds serves not only as a fibrous structure for cell adhesion and growth in tissue engineering, but can also provide the controlled release of drugs and other molecules for biomedical applications. There has been a limited number of reports on the use of scaffold materials in biomedical sensing applications. This review highlights the potential of scaffold materials in the improvement of sensing platforms and summarizes the progress in the application of novel scaffold-based materials as sensor, and discusses their advantages and limitations. Furthermore, the influence of the scaffold materials on the monitoring of infectious diseases such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and bacterial infections, was reviewed.

show abstract

CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor

Cited by 314 publications

References 33 publications

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity

Application of MXene in Electrochemical Sensors: A Review

Applications of scaffold-based advanced materials in biomedical sensing

Contact Info

Product

Resources

About