CRISPR/Cas12a-powered evanescent wave fluorescence nanobiosensing platform for nucleic acid amplification-free detection of Staphylococcus aureus with multiple signal enhancements

Song, Dan; Xu, Wenjuan; Han, Xiangzhi; Wang, Hongliang; Zhuo, Yuxin; Liu, Jiayuan; Zhu, Anna; Long, Feng

doi:10.1016/j.bios.2023.115109

Cited by 18 publications

(12 citation statements)

References 43 publications

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“…Additionally, CREAT utilizes strategies such as Mg 2+ or DNA-mediated fluorescence enhancement and air displacement fluorescence enhancement. The platform is stable, easy to operate, and makes CRISPR/Cas12a-based research more appealing …”

Section: Mds-based Biosensor Platformmentioning

confidence: 99%

“…The platform is stable, easy to operate, and makes CRISPR/ Cas12a-based research more appealing. 49 Ming et al have first established a third promising CRISPR system for plant genome engineering: CRISPR-Cas12b, which improves the efficiency of gene activation, providing a wider range of targeting sites for gene suppression. 50 Like Cas12a, it identifies T-rich PAMs upstream of the target area in the dsDNA substrate.…”

Section: ■ Microbial Defense Systemmentioning

confidence: 99%

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CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

Yang,

Mao,

Wang

et al. 2023

ACS Sens.

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Over the past few decades, pathogens have posed a threat to human security, and rapid identification of pathogens should be one of the ideal methods to prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive and specific approaches to identify and quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems and Argonaute (Ago) belong to the Microbial Defense Systems (MDS). The guided, programmable, and targeted activation of nucleases by both of them is leading the way to a new generation of pathogens detection. We compare these two nucleases in terms of similarities and differences. In addition, we discuss future challenges and prospects for the development of the CRISPR/Cas systems and Argonaute (Ago) biosensors, especially electrochemical biosensors. This review is expected to afford researchers entering this multidisciplinary field useful guidance and to provide inspiration for the development of more innovative electrochemical biosensors for pathogens detection.

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Section: Mds-based Biosensor Platformmentioning

confidence: 99%

Section: ■ Microbial Defense Systemmentioning

confidence: 99%

CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

Yang,

Mao,

Wang

et al. 2023

ACS Sens.

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“…An evanescent wave fluorescence nanobiosensing platform based on CRISPR/Cas12a was developed for the ultrasensitive detection of Staphylococcus aureus. (LOD = 13.2 CFU/mL) ( Figure 17 ) [ 120 ].…”

Section: Sensing Mechanismsmentioning

confidence: 99%

“… A CRISPR/Cas12a-powered FOEW sensor for nucleic acid amplification-free detection of Staphylococcus aureus with multiple signal enhancements. Reprinted from Song et al (2023) [ 120 ], Copyright (2023), with permission from Elsevier. …”

Section: Figurementioning

confidence: 99%

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

Wang

Lou

2023

Biosensors

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Biosensors capable of onsite and continuous detection of environmental and food pollutants and biomarkers are highly desired, but only a few sensing platforms meet the “2-SAR” requirements (sensitivity, specificity, affordability, automation, rapidity, and reusability). A fiber optic evanescent wave (FOEW) sensor is an attractive type of portable device that has the advantages of high sensitivity, low cost, good reusability, and long-term stability. By utilizing functional nucleic acids (FNAs) such as aptamers, DNAzymes, and rational designed nucleic acid probes as specific recognition ligands, the FOEW sensor has been demonstrated to be a general sensing platform for the onsite and continuous detection of various targets ranging from small molecules and heavy metal ions to proteins, nucleic acids, and pathogens. In this review, we cover the progress of the fluorescent FNA-based FOEW biosensor since its first report in 1995. We focus on the chemical modification of the optical fiber and the sensing mechanisms for the five above-mentioned types of targets. The challenges and prospects on the isolation of high-quality aptamers, reagent-free detection, long-term stability under application conditions, and high throughput are also included in this review to highlight the future trends for the development of FOEW biosensors capable of onsite and continuous detection.

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“…Human health is facing the threat of bacterial infections, despite impressive developments in diagnostic and therapeutic techniques. − Staphylococcus aureus (S. aureus) is a Gram-positive pathogen widely distributed in air, water, and dust. − A low content of S.…”

Section: Introductionmentioning

confidence: 99%

Dual-Modal Biosensor for Staphylococcus aureus Detection Based on a Porphyrin-Based Porous Organic Polymer FePor-TPA with Excellent Peroxidase-like, Catalase-like, and Photoelectrochemical Properties

Zheng,

Ma,

et al. 2023

Anal. Chem.

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Bacterial infections seriously harm human health and cause many severe diseases, which triggered urgent demands to exploit specific and sensitive biosensor strategies for Staphylococcus aureus detection. Here, a colorimetric and photoelectrochemical dual-mode biosensor for S. aureus assay based on FePor-TPA was constructed. 2D FePor-TPA thin film and its bulk powder (FePor-TPA) were synthesized by in situ growth on ITO and a solvothermal condition, respectively, both of which exhibited excellent peroxidase-like and catalase-like activity, originating from their metalloporphyrin linkers. Benefiting from the in situ growth on ITO electrodes, the 2D FePor-TPA thin film also possessed a more ordered stacking mode and in turn exhibited good electrical conductivity, stable initial photocurrent, and high sensitivity to O2. As for bulk FePor-TPA, its porous structure and high specific surface area make it a possible scaffold to load an amount of AuNPs, the rabbit anti-Staphylococcus aureus Rosenbach tropina antibody (Ab2), and GOx for constructing the signal probe (GOx/Ab2@Au@FePor-TPA) and realizing catalytic amplification. With these satisfactory features in mind, the 2D FePor-TPA thin film and its bulk powder (FePor-TPA) were utilized to construct a dual and signal-on bioplatform for sensitively and selectively detecting S. aureus, which, as far as we know, has not been reported.

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CRISPR/Cas12a-powered evanescent wave fluorescence nanobiosensing platform for nucleic acid amplification-free detection of Staphylococcus aureus with multiple signal enhancements

Cited by 18 publications

References 43 publications

CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

Dual-Modal Biosensor for Staphylococcus aureus Detection Based on a Porphyrin-Based Porous Organic Polymer FePor-TPA with Excellent Peroxidase-like, Catalase-like, and Photoelectrochemical Properties

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