CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for β-carotene biosynthesis in banana fruit

Kaur, Navneet; Alok, Anshu; Shivani, .; Kumar, Pankaj; Kaur, Navjot; Awasthi, Praveen; Chaturvedi, Siddhant; Pandey, Pankaj; Pandey, Ashutosh; Pandey, Ajay Kumar; Tiwari, Siddharth

doi:10.1016/j.ymben.2020.01.008

Cited by 186 publications

(83 citation statements)

References 52 publications

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“…The dysfunction of the genes involved in the desaturation process leads to the deficiency of carotenoid accumulation and the death of the plants (Wang M. et al, 2009). The unique phenomenon of pale leaves and lethal phenotype of the plants, which caused by inhibiting the desaturation process, have been usually used to evaluate the efficiency of genetic modification technology (Kaur et al, 2018). Meanwhile, carotenoids are also used as nutrient supplements (Leelakanok et al, 2018) and chemotherapy reagents (Noto et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, carotenoids are also used as nutrient supplements (Leelakanok et al, 2018) and chemotherapy reagents (Noto et al, 1996). Genes involved in the carotenoid biosynthesis have been isolated and identified for enzymatic activities analysis (Norris et al, 1995;Liu et al, 2013;Brausemann et al, 2017), nutritional enhancements of food (Wurtzel et al, 2003) and genetical modification technology tools (Velasquez et al, 2009;Kaur et al, 2018). Recently, some studies expounded that the over-expression increased the carotenoid content and improved the tolerance to abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

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Over-Expression of LcPDS, LcZDS, and LcCRTISO, Genes From Wolfberry for Carotenoid Biosynthesis, Enhanced Carotenoid Accumulation, and Salt Tolerance in Tobacco

Wang

et al. 2020

Front. Plant Sci.

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It is of great importance to combine stress tolerance and plant quality for breeding research. In this study, the role of phytoene desaturase (PDS), z-carotene desaturase (ZDS) and carotene isomerase (CRTISO) in the carotenoid biosynthesis are correlated and compared. The three genes were derived from Lycium chinenses and involved in the desaturation of tetraterpene. Their over-expression significantly increased carotenoid accumulation and enhanced photosynthesis and salt tolerance in transgenic tobacco. Up-regulation of almost all the genes involved in the carotenoid biosynthesis pathway and only significant down-regulation of lycopene e-cyclase (e-LCY) gene were detected in those transgenic plants. Under salt stress, proline content, and activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) were significantly increased, whereas malonaldehyde (MDA) and hydrogen peroxide (H 2 O 2) accumulated less in the transgenic plants. The genes encoding ascorbate peroxidase (APX), CAT, POD, SOD, and pyrroline-5-carboxylate reductase (P5CR) were shown to responsive up-regulated significantly under the salt stress in the transgenic plants. This study indicated that LcPDS, LcZDS, and LcCRTISO have the potential to improve carotenoid content and salt tolerance in higher plant breeding.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Over-Expression of LcPDS, LcZDS, and LcCRTISO, Genes From Wolfberry for Carotenoid Biosynthesis, Enhanced Carotenoid Accumulation, and Salt Tolerance in Tobacco

Wang

et al. 2020

Front. Plant Sci.

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“…To date, the main traits modified through gene editing are related to the albino phenotype, which is used as a proof-ofconcept for established protocols, since it allows early phenotype screening. The phytoene desaturase (PDS) gene has been modified in many species, such as apple (Nishitani et al, 2016), pear (Charrier et al, 2019), banana (Kaur et al, 2018;Naim et al, 2018), grape (Nakajima et al, 2017;Ren et al, 2019), kiwifruit (Wang et al, 2018), strawberry (Wilson et al, 2019a), and citrus (Jia et al, 2017a;Zhang et al, 2017;Zhu et al, 2019). In addition to the albino trait, gene editing has been used to induce resistance to important pathogens for some fruit tree species ( Figure 2).…”

Section: Techniques and Methodsologies For Genome Editingmentioning

confidence: 99%

New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

et al. 2020

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New plant breeding techniques (NPBTs) aim to overcome traditional breeding limits for fruit tree species, in order to obtain new varieties with improved organoleptic traits and resistance to biotic and abiotic stress, and to maintain fruit quality achieved over centuries by (clonal) selection. Knowledge on the gene(s) controlling a specific trait is essential for the use of NPBTs, such as genome editing and cisgenesis. In the framework of the international scientific community working on fruit tree species, including citrus, NPBTs have mainly been applied to address pathogen threats. Citrus could take advantage of NPBTs because of its complex species biology (seedlessness, apomixis, high heterozygosity, and long juvenility phase) and aptitude for in vitro manipulation. To our knowledge, genome editing in citrus via transgenesis has successful for induced resistance to Citrus bacterial canker in sweet orange and grapefruit using the resistance gene CsLOB1. In the future, NPBTs will also be used to improve fruit traits, making them healthier. The regeneration of plants following the application of NPBTs is a bottleneck, making it necessary to optimize the efficiency of current protocols. The strengths and weaknesses of using explants from young in vitro plantlets, and from mature plants, will be discussed. Other major issues addressed in this review are related to the requirement for marker-free systems and shortening the long juvenility phase. This review aims to summarize methods and approaches available in the literature that are suitable to citrus, focusing on the principles observed before the use of NPBTs.

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“…These initial reports open a new avenue for gene‐editing attempts in banana. In a recent report, the β‐carotene‐enriched banana was developed by CRISPR/Cas9‐based editing of lycopene epsilon‐cyclase gene in the Cavendish banana cultivar (Kaur et al., 2020). In addition to generating material for breeding in banana, our group is trying to develop banana cultivar using the CRISPR/Cas9 tool with the core aim for the nutritional enhancement of banana fruit in high yielding and lower nutrient containing popular cultivar in India.…”

Section: Gene Editing and Improvement Of Cereals And Horticultural Cropsmentioning

confidence: 99%

Emerging tools and paradigm shift of gene editing in cereals, fruits, and horticultural crops for enhancing nutritional value and food security

Tiwari

Trivedi

Pandey

2020

Food and Energy Security

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Gene editing using sequence‐specific nucleases, particularly CRISPR/Cas ribonucleoprotein, has drawn enormous attention in plant research in recent years. Nearly a decade ago, Cas9 protein was initially discovered for a role in adaptive immunity in bacteria. Owing to vast potential, a large number of reports came out in a short span of time, comprising the identification of Cas protein from different bacterial sources, new Cas9 variants with reduced off‐targets, multiplexing, base editing, prime editing, and RNA manipulation in plants. Studies revealed that CRISPR/Cas‐based gene editing can play a major role in ensuring food security via developing resilient commercial crops with improved yield and nutritional value. Use of the CRISPR/Cas9 system for creating mutation in genes and regulatory regions of promoter generated a number of alleles with variable phenotypes, which can serve as an excellent genetic resource in the breeding program. In this review, we provide a recent overview of state‐of‐art discoveries in the CRISPR/Cas system comprised of new Cas proteins, modifications of existing Cas9, refinements in CRISPR/Cas‐induced gene editing, applications, and outcome emphasizing on major cereals and horticultural crops. We also highlight the current global policy framework for the regulation of gene‐edited crops.

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CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for β-carotene biosynthesis in banana fruit

Cited by 186 publications

References 52 publications

Over-Expression of LcPDS, LcZDS, and LcCRTISO, Genes From Wolfberry for Carotenoid Biosynthesis, Enhanced Carotenoid Accumulation, and Salt Tolerance in Tobacco

Over-Expression of LcPDS, LcZDS, and LcCRTISO, Genes From Wolfberry for Carotenoid Biosynthesis, Enhanced Carotenoid Accumulation, and Salt Tolerance in Tobacco

New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review

Emerging tools and paradigm shift of gene editing in cereals, fruits, and horticultural crops for enhancing nutritional value and food security

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