CRISPR/Cas9 editing of wheat Ppd-1 gene homoeologs alters spike architecture and grain morphometric traits

Errum, Aliya; Rehman, Nazia; Uzair, Muhammad; Inam, Safeena; Ali, Ghulam Muhammad; Khan, Muhammad Ramzan

doi:10.1007/s10142-023-00989-2

Cited by 14 publications

(2 citation statements)

References 70 publications

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“…The photoperiodic flowering pathway is mainly regulated by a PRR gene, known as Photoperiod-H1 ( Ppd-H1 ) in barley and PHOTOPERIOD1 ( PPD1 ) in wheat, which activate VRN-H3/VRN3 expression and consequently promote flowering ( Turner et al 2005 ; Beales et al 2007 ). In addition, these PRR genes also control agronomic traits such as leaf size and inflorescence architecture in barley and wheat, respectively ( Boden et al 2015 ; Digel et al 2016 ; Errum et al 2023 ).…”

Section: Photoperiodic Regulation By Co and ...mentioning

confidence: 99%

CONSTANS, a HUB for all seasons: How photoperiod pervades plant physiology regulatory circuits

Romero,

Serrano-Bueno,

Camacho-Fernández

et al. 2024

The Plant Cell

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How does a plant detect the changing seasons and make important developmental decisions accordingly? How do they incorporate daylength information into their routine physiological processes? Photoperiodism, or the capacity to measure the daylength, is a crucial aspect of plant development that helps plants determine the best time of the year to make vital decisions, such as flowering. The protein CONSTANS (CO) constitutes the central regulator of this sensing mechanism, not only activating florigen production in the leaves but also participating in many physiological aspects in which seasonality is important. Recent discoveries place CO in the center of a gene network that can determine the length of the day and confer seasonal input to aspects of plant development and physiology as important as senescence, seed size, or circadian rhythms. In this review, we discuss the importance of CO protein structure, function, and evolutionary mechanisms that embryophytes have developed to incorporate annual information into their physiology.

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Section: Photoperiodic Regulation By Co and ...mentioning

confidence: 99%

CONSTANS, a HUB for all seasons: How photoperiod pervades plant physiology regulatory circuits

Romero,

Serrano-Bueno,

Camacho-Fernández

et al. 2024

The Plant Cell

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“…Numerous studies investigated the impact of Ppd-1 genes on various aspects of plant development. It has been found that Ppd-1 genes strongly influence on yield-related traits in wheat, including the number of kernel-producing spikelets, spike length, plant height, leaf size, thousand grain weight and spike fertility (Tanio and Kato 2007 ; Seki et al 2011 , 2013 ; Langer et al 2014 ; Kiss et al 2014 ; Cho et al 2015 ; Zhang et al 2015 ; Boden et al 2015 ; Grogan et al 2016 ; Steinfort et al 2017b ; Jones et al 2017 ; Prieto et al 2018 ; Ramirez et al 2018 ; Arjona et al 2018 , 2020 ; Ochagavía et al 2018 ; Pérez-Gianmarco et al 2019 , 2020 ; Sun et al 2020 ; Amo et al 2022 ; Fait and Balashova 2022 ; Errum et al 2023 ). However, the majority of these studies focused on the impact of insensitive and sensitive alleles on agronomic traits, identifying structural variations within the Ppd-1 region in bread wheat populations using PCR-based methods to detect fragment size differences.…”

Section: Introductionmentioning

confidence: 99%

Novel PHOTOPERIOD-1 gene variants associate with yield-related and root-angle traits in European bread wheat

Makhoul,

Schlichtermann,

Ugwuanyi

et al. 2024

Theor Appl Genet

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Key message PHOTOPERIOD-1 homoeologous gene copies play a pivotal role in regulation of flowering time in wheat. Here, we show that their influence also extends to spike and shoot architecture and even impacts root development. Abstract The sequence diversity of three homoeologous copies of the PHOTOPERIOD-1 gene in European winter wheat was analyzed by Oxford Nanopore amplicon-based multiplex sequencing and molecular markers in a panel of 194 cultivars representing breeding progress over the past 5 decades. A strong, consistent association with an average 8% increase in grain yield was observed for the PpdA1-Hap1 haplotype across multiple environments. This haplotype was found to be linked in 51% of cultivars to the 2NS/2AS translocation, originally introduced from Aegilops ventricosa, which leads to an overestimation of its effect. However, even in cultivars without the 2NS/2AS translocation, PpdA1-Hap1 was significantly associated with increased grain yield, kernel per spike and kernel per m2 under optimal growth conditions, conferring a 4% yield advantage compared to haplotype PpdA1-Hap4. In contrast to Ppd-B1 and Ppd-D1, the Ppd-A1 gene exhibits novel structural variations and a high number of SNPs, highlighting the evolutionary changes that have occurred in this region over the course of wheat breeding history. Additionally, cultivars carrying the photoperiod-insensitive Ppd-D1a allele not only exhibit earlier heading, but also deeper roots compared to those with photoperiod-sensitive alleles under German conditions. PCR and KASP assays have been developed that can be effectively employed in marker-assisted breeding programs to introduce these favorable haplotypes.

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Precise Gene Editing of Cereals Using CRISPR/Cas Technology

Pouramini,

Hensel

2023

A Roadmap for Plant Genome Editing

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Targeted mutagenesis using CRISPR/Cas technology has become routine in elucidating biological processes or their application in breeding and agriculture. This means that the change to be achieved can be accurately predicted. However, knockout of a gene function is not always desirable, as reducing activity or affecting a protein domain can influence its properties and, thus, the phenotype. This chapter will therefore focus on precise genome modification in temperate cereals. The methods used, including some representative examples, are summarised here.

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CRISPR/Cas9 editing of wheat Ppd-1 gene homoeologs alters spike architecture and grain morphometric traits

Cited by 14 publications

References 70 publications

CONSTANS, a HUB for all seasons: How photoperiod pervades plant physiology regulatory circuits

CONSTANS, a HUB for all seasons: How photoperiod pervades plant physiology regulatory circuits

Novel PHOTOPERIOD-1 gene variants associate with yield-related and root-angle traits in European bread wheat

Precise Gene Editing of Cereals Using CRISPR/Cas Technology

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