CRISPR/Cas9 genome editing through<i>in planta</i>transformation

Zlobin, Nikolay; Lebedeva, Marina; Taranov, Vasiliy

doi:10.1080/07388551.2019.1709795

Cited by 28 publications

(18 citation statements)

References 118 publications

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“…For the biolistic delivery, gold particles coated with plasmids expressing CRISPR/Cas9 components were bombarded into SAM-exposed embryos of imbibed seeds and mutations were achieved [39]. In Arabidopsis, the easiest and most commonly used method of in planta transformation is the floral-dip [40]. is technique is based on the immersion of unopened flowers into an Agrobacterium suspension containing a strong surfactant, generally Silwet-77.…”

Section: Methods To Improve the Delivery Of Crispr/ Cas9 In Plantsmentioning

confidence: 99%

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The Application of CRISPR/Cas9 Technology in the Management of Genetic and Nongenetic Plant Traits

Mutezo

Sedibe

Mofokeng

et al. 2021

International Journal of Agronomy

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Gene editing (GE) has yielded positive results in the management of genetic and nongenetic plant traits. Clustered interspaced palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (together known as the CRISPR/Cas system) are relatively easy to use, compared to other existing genome editing technologies. The CRISPR/Cas tool produces unchanging gene mutations, with the ability to segregate from the Cas9/sgRNA construct to avoid similar modifications by CRISPR/Cas. CRISPR/Cas GE is fast and accurate; crops developed using this technique are resistant to viruses, fungi, and bacteria and resilient to abiotic and biotic stresses, while crops established using conventional methods take between 10 and 15 years to develop resistance. Therefore, CRISPR/Cas is a useful tool for sustainable agricultural production. Plant traits have been successfully manipulated using this technology. Notwithstanding the technical challenges of transferring CRISPR/Cas9 developed crops from the laboratory to the field, additional obstacles include uncertain policy systems, dispute over intellectual property ownership, and acceptability by consumers. Several Cas9-based applied techniques have gained popularity that enable researchers to enhance plants with speed and accuracy. In conclusion, the CRISPR/Cas9 system is a powerful technology for genetically modifying crops.

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Section: Methods To Improve the Delivery Of Crispr/ Cas9 In Plantsmentioning

confidence: 99%

“…Following fertilization, zygotes with inserted T-DNA give rise to transgenic embryos and, after germination, to transgenic plants. is method makes it very easy to generate transgenic plants, without going through in vitro mechanisms [40].…”

Section: Methods To Improve the Delivery Of Crispr/ Cas9 In Plantsmentioning

confidence: 99%

The Application of CRISPR/Cas9 Technology in the Management of Genetic and Nongenetic Plant Traits

Mutezo

Sedibe

Mofokeng

et al. 2021

International Journal of Agronomy

View full text Add to dashboard Cite

show abstract

“…In planta transformation methods can be particularly useful because no in vitro regeneration of shoots or embryos is required. It is preferred that morphogenic regulator genes are not used or that the morphogenic regulator genes could be excised out of the genome by inducible recombinase excision system, because expression of these transgenes can affect plant growth and development [139][140][141].…”

Section: Biodesign Researchmentioning

confidence: 99%

Plant Biosystems Design Research Roadmap 1.0

et al. 2020

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Human life intimately depends on plants for food, biomaterials, health, energy, and a sustainable environment. Various plants have been genetically improved mostly through breeding, along with limited modification via genetic engineering, yet they are still not able to meet the ever-increasing needs, in terms of both quantity and quality, resulting from the rapid increase in world population and expected standards of living. A step change that may address these challenges would be to expand the potential of plants using biosystems design approaches. This represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological systems. Plant biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant genomes. From this perspective, we present a comprehensive roadmap of plant biosystems design covering theories, principles, and technical methods, along with potential applications in basic and applied plant biology research. We highlight current challenges, future opportunities, and research priorities, along with a framework for international collaboration, towards rapid advancement of this emerging interdisciplinary area of research. Finally, we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception, trust, and acceptance.

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“…Introduction of genetic changes into meristems reported concomitant transgenic expression in the shoots which later on produced flowers and seeds, ultimately transmitting transgenes and gene edits to the next generation (Maher et al, 2020). Agrobacterium mediated in planta transformation approaches using apical meristem has been the preferred target for successful develoyment of transgenics as reported by most of the recent studies (Maher et al, 2020;Tyagi et al, 2020;Zlobin et al, 2020).…”

Section: Tablementioning

confidence: 99%

Current Status of Transgenic Cotton-Utility of Genotype Independent in Planta Approaches for the Generation of Transgenics

Kesiraju¹

2020

International Journal on Agricultural Sciences

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Cotton (Gossypium spp.), is a mercantile crop plant is grown for its fluffy fiber and cotton seed oil in around 70 countries worldwide. Cotton is an economically important crop, shows erratic productivity under rain feed conditions; it is bogged down with many biotic and abiotic stresses. Due to lack of resistant germplasm, crop improvement through conventional breeding practices has been lagging. Genetic engineering offers numerous protocols to engineer plants to overcome stress. Biotechnological intervention for cotton improvement has begun three decades ago. The recalcitrance of cotton to tissue culture has been the major constraint for in vitro regeneration. Alternate methods that evade tissue culture regeneration steps have thus been envisaged. Till date there are very few standardized protocols that can be employed to develop transgenics in a genotype independent manner. Thus, genotype independent in planta transformation strategies have gained momentum in the present days, but reproducibility of reported protocols remains an amigna in many cases. In planta transformations holds prominence due to viability and ease in generation of transgenic cotton plants with in less time. This review focuses on grouping efforts made by different research groups in this senior. Several reports and standardizations have been focused that reports development of transgenic cotton.

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CRISPR/Cas9 genome editing throughin plantatransformation

Cited by 28 publications

References 118 publications

The Application of CRISPR/Cas9 Technology in the Management of Genetic and Nongenetic Plant Traits

The Application of CRISPR/Cas9 Technology in the Management of Genetic and Nongenetic Plant Traits

Plant Biosystems Design Research Roadmap 1.0

Current Status of Transgenic Cotton-Utility of Genotype Independent in Planta Approaches for the Generation of Transgenics

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