CRISPR/Cas9 mediated dual fluorescent reporter mice to study spatial bone genomics of osteoblasts and osteoclasts in the local in vivo environment

Yilmaz, Dilara; Fischer, Yannick; Zimmermann, Sandra; Hwang, Gaonhae; Müller, Ralph; Wehrle, Esther

doi:10.1016/j.bonr.2021.100871

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our lab has previously established a bone cell reporter (BCR) mouse model using CRISPR/Cas9 technology to label osteoblast (Integrin binding sialoprotein -Ibsp) and osteoclast-specific targets (tartrate-resistant acid phosphatase (TRAP) type 5 -Acp-5) with fluorescent proteins (eGFP, mCherry) (75). All mice were bred, monitored and maintained under specific pathogen free (SPF) conditions at the ETH Phenomics Centre, ETH Zürich (12 h:12 h light-dark cycle, ad libitum access to maintenance feed and water).…”

Section: Mouse Linementioning

confidence: 99%

Spatial Transcriptomics in Bone Mechanomics: Exploring the Mechanoregulation of Fracture Healing in the Era of Spatial Omics

Mathavan,

Singh,

Correia Marques

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

In over 100 years, the field of bone mechanobiology has sought experimental techniques to unravel the molecular mechanisms governing the phenomenon of mechanically-regulated bone (re)modelling. Each cell within a fracture site resides within different local micro-environments characterized by different levels of mechanical strain - thus, preserving the spatial location of each cell is critical in relating cellular responses to mechanical stimuli. Our spatial transcriptomics based "mechanomics" platform facilitates spatially-resolved analysis of the molecular profiles of cells with respect to their local in vivo mechanical environment by integrating time-lapsed in vivo micro-computed tomography, spatial transcriptomics, and micro-finite element analysis. We investigate the transcriptomic responses of cells as a function of the local strain magnitude by identifying the differential expression of genes in regions of high and low strain within a fracture site. Our platform thus has the potential to address fundamental open questions within the field and identify novel mechano-responsive targets to enhance bone regeneration.

show abstract

Section: Mouse Linementioning

confidence: 99%