2022
DOI: 10.1101/2022.09.26.509268
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CRISPR/Cas9 mediated gene editing in non-model nematodePanagrolaimussp. PS1159

Abstract: The phylum Nematoda consists of a huge diversity of species in a broad range of ecosystems and habitats. Nematodes share a largely conserved Bauplan but major differences have been found in early developmental processes. The development of the nematode model organism Caenorhabditis elegans has been studied in great detail for decades. These efforts have provided the community with a large number of protocols and methods. Unfortunately, many of these tools are not easily applicable in non-Caenorhabditis nematod… Show more

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“…RNA interference (RNAi) that could for functional genomic analysis has been succesful in panagrolaimids (Shannon, Tyson, Dix, Boyd, & Burnell, 2008). Recent studies have also performed gene editing in the genus mediated by CRISPR/Cas9 (Hellekes et al, 2023). Their varied reproductive modes, have allowed for the study of the evolution of parthenogenesis (Schiffer et al, 2019) (Shatilovich et al, 2023).…”
Section: Introductionmentioning
confidence: 99%
“…RNA interference (RNAi) that could for functional genomic analysis has been succesful in panagrolaimids (Shannon, Tyson, Dix, Boyd, & Burnell, 2008). Recent studies have also performed gene editing in the genus mediated by CRISPR/Cas9 (Hellekes et al, 2023). Their varied reproductive modes, have allowed for the study of the evolution of parthenogenesis (Schiffer et al, 2019) (Shatilovich et al, 2023).…”
Section: Introductionmentioning
confidence: 99%