CRISPR/Cas9-mediated gene editing of the let-7 seed sequence improves silk yield in the silkworm, Bombyx mori

Wang, Wei; Zhang, Fan; Guo, Kaiyu; Xu, Jianping; Zhao, Ping; Xia, Qingyou

doi:10.1016/j.ijbiomac.2023.124793

Cited by 11 publications

(2 citation statements)

References 52 publications

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“…In B. mori, the delivery of the CRISPR/Cas9 system based on plasmids was quick and convenient, with high stability in the silkworms [30][31][32][33]. However, Cas9-and sgRNApositive individuals were only available in the G 1 generation, and the gene-edited individuals were generated in the hybrid offspring of Cas9-and sgRNA-positive individuals.…”

Section: Discussionmentioning

confidence: 99%

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Yu,

Dong,

Jiang

et al. 2023

Insects

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Lepidoptera is one of the most speciose insect orders, causing enormous damage to agricultural and forest crops. Although genome editing has been achieved in a few Lepidoptera for insect controls, most techniques are still limited. Here, by injecting female pupae of the Lepidoptera model species, Bombyx mori, gene editing was established using the Receptor-Mediated Ovary Transduction of Cargo (ReMOT) control technique. We identified a B. mori oocytes-targeting peptide ligand (BmOTP, a 29 aa of vitellogenin N-terminal of silkworms) with a highly conserved sequence in lepidopteran insects that could efficiently deliver mCherry into oocytes. When BmOTP was fused to CRISPR-associated protein 9 (Cas9) and the BmOTP-Cas9 ribonucleoprotein complex was injected into female pupae, heritable editing of the offspring was achieved in the silkworms. Compared with embryo microinjection, individual injection is more convenient and eliminates the challenge of injecting extremely small embryos. Our results will significantly facilitate the genetic manipulation of other lepidopteran insects, which is essential for advancing lepidopteran pest control.

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Section: Discussionmentioning

confidence: 99%

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Yu,

Dong,

Jiang

et al. 2023

Insects

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“…Because differences were observed between male and female individuals, fifth-instar males were selected for daily detailed investigation [17][18][19]. Silkworms fed the different diets are shown in Figure 2B,C.…”

Section: Effect Of Valine On Feed Efficiency Of Silkworm Bombyx Morimentioning

confidence: 99%

Silkworm Hemolymph and Cocoon Metabolomics Reveals Valine Improves Feed Efficiency of Silkworm Artificial Diet

Wu,

Li,

Qin

et al. 2024

Insects

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Artificial silkworm diets significantly impact farm profitability. Sustainable cocoon production depends on the continuous improvement of feed efficiency to reduce costs and nutrient losses in the feed. This study used metabolomics to explore the differences in silkworm cocoons and hemolymph under two modes of rearing: an artificial diet and a mulberry-leaf diet. Nine metabolites of silkworm cocoons and hemolymph in the mulberry-leaf group were higher than those in the artificial-diet group. Enrichment analysis of the KEGG pathways for these metabolites revealed that they were mainly enriched in the valine, leucine, and isoleucine biosynthesis and degradation pathways. Hence, the artificial silkworm diet was supplemented various concentrations of valine were supplemented to with the aim of examining the impact of valine on their feeding and digestion of the artificial diet. The results indicated that valine addition had no significant effect on feed digestibility in the fifth-instar silkworm. Food intake in the 2% and 4% valine groups was significantly lower than that in the 0% valine group. However, the 2% and 4% valine groups showed significantly improved cocoon-production efficiency, at 11.3% and 25.1% higher, respectively. However, the cocoon-layer-production efficiencies of the 2% and 4% valine groups decreased by 7.7% and 13.9%, respectively. The research confirmed that valine is an effective substance for enhancing the feed efficiency of silkworms.

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Genome editing: A novel approach to manage insect vectors of plant viruses

Jangra,

Potts,

Ghosh

et al. 2024

Insect Biochemistry and Molecular Biology

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CRISPR/Cas9-mediated gene editing of the let-7 seed sequence improves silk yield in the silkworm, Bombyx mori

Cited by 11 publications

References 52 publications

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control in Bombyx mori

Silkworm Hemolymph and Cocoon Metabolomics Reveals Valine Improves Feed Efficiency of Silkworm Artificial Diet

Genome editing: A novel approach to manage insect vectors of plant viruses

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