CRISPR/Cas9 mediated gene knockout reveals a more important role of PBP1 than PBP2 in the perception of female sex pheromone components in Spodoptera litura

Zhu, Guan-Heng; Zheng, Mei-Yan; Sun, Jin; Khuhro, Sajjad Ali; Yan, Qi; Huang, Yongping; Syed, Zainulabeuddin; Dong, Shuang-Lin

doi:10.1016/j.ibmb.2019.103244

Cited by 51 publications

(38 citation statements)

References 47 publications

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“…IRs from D. melanogaster (Diptera) are labeled in dark blue, IRs from B. mori (Lepidoptera) are labeled in purple, IRs from M. sexta (Lepidoptera) are labeled in green, and IRs from A. mellifera (Hymenoptera) are labeled in light blue. Jin et al, 2014;Chang et al, 2015;Zhu et al, 2016Zhu et al, , 2019. Ten genes (CsacOBP7/8/10/13/14/16/18/20/21/28) without significant differences in expression levels between males and females may function as general odourant detectors rather than in pheromone recognition (Li et al, 2008;Pelletier and Leal, 2009;He et al, 2019a).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Characterization and Expression Analysis of Chemosensory Genes in Chilo sacchariphagus (Lepidoptera Crambidae), a Key Pest of Sugarcane

Liu

et al. 2021

Front. Physiol.

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Insect chemoreception involves many families of genes, including odourant/pheromone binding proteins (OBP/PBPs), chemosensory proteins (CSPs), odourant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs), which play irreplaceable roles in mediating insect behaviors such as host location, foraging, mating, oviposition, and avoidance of danger. However, little is known about the molecular mechanism of olfactory reception in Chilo sacchariphagus, which is a major pest of sugarcane. A set of 72 candidate chemosensory genes, including 31 OBPs/PBPs, 15 CSPs, 11 ORs, 13 IRs, and two SNMPs, were identified in four transcriptomes from different tissues and genders of C. sacchariphagus. Phylogenetic analysis was conducted on gene families and paralogs from other model insect species. Quantitative real-time PCR (qRT-PCR) showed that most of these chemosensory genes exhibited antennae-biased expression, but some had high expression in bodies. Most of the identified chemosensory genes were likely involved in chemoreception. This study provides a molecular foundation for the function of chemosensory proteins, and an opportunity for understanding how C. sacchariphagus behaviors are mediated via chemical cues. This research might facilitate the discovery of novel strategies for pest management in agricultural ecosystems.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Characterization and Expression Analysis of Chemosensory Genes in Chilo sacchariphagus (Lepidoptera Crambidae), a Key Pest of Sugarcane

Liu

et al. 2021

Front. Physiol.

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“…Recently, OBPs are considered potential molecular targets for developing RNA interference (RNAi)- and genome editing-based strategies for pest management. For instance, knockdown of OBP genes by RNAi impairs olfactory sensitivity in Adelphocoris lineolatus (Goeze) (Hemiptera: Miridae) and Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) ( Dong et al 2017 , Zhang et al 2017 ), and deletion of OBP s by CRISPR/Cas9 technology significantly reduces the olfactory response in H. armigera and Spodoptera litura Fabricius (Lepidoptera: Noctuidae) ( Ye et al 2017 , Zhu et al 2019 ).…”

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confidence: 99%

Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

Jiang

et al. 2020

Journal of Insect Science

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The small white butterfly, Pieris rapae (L.), is an important insect pest of Brassica crops. This species utilize olfactory cues to find their hosts and mates. However, the molecular mechanism underlying the olfactory perception in this species remains unclear. Here, we identified 14 odorant-binding proteins (OBP) genes—essential for insect olfaction—in P. rapae by exploring a previously published transcriptome dataset. Proteins encoded by all of these genes contain N-terminal signal peptides and six positionally conserved cysteine residues, which are characteristic of insect OBPs. These OBPs displayed high amino acid identity with their respective orthologs in other lepidopterans, and several conserved motifs were identified within these OBPs. Phylogenetic analysis showed that these OBPs were well segregated from each other and clustered into different branches. PrapOBP1 and PrapOBP2 were clustered into the ‘general odorant-binding protein’ clade, and PrapOBP3 and PrapOBP4 fall into the ‘pheromone-binding protein’ clade. The 14 OBP genes were located on seven genomic scaffolds. Of these, PrapOBP1, 2, 3, and 4 were located on scaffold332, whereas PrapOBP5, 6, 7, 8, and 9 were located on scaffold116. Ten of the 14 genes had antenna-biased expression. Of these, PrapOBP1, 2, 4, and 13 were enriched in male antennae, whereas PrapOBP7 and PrapOBP10 were female-biased. Our findings suggest that these OBPs may be involved in olfactory communication. To the best of our knowledge, this is the first report on the identification and characterization of OBPs in P. rapae, and our findings provide a solid foundation for studying the functions of these genes.

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“…In the second hypothesis, OBPs have been suggested to maintain the stability of the insect olfactory system by buffering the effects of sudden changes caused by odorant stimuli, rather than activating the receptor, while the odorants can directly activate the ORs [113]. Notably, a recent study has shown that CRISPR/Cas9-mediated knockout of PBP genes in the tobacco cutworm Spodoptera litura reduced antennal and behavioral responses to sex pheromone components, but did not functionally abolish them [114], which supports the idea that OBPs act as mediators for the transport of hydrophobic ligands in the sensillum lymph.…”

Section: Obpsmentioning

confidence: 99%

Recent Advancements in Studies on Chemosensory Mechanisms Underlying Detection of Semiochemicals in Dacini Fruit Flies of Economic Importance (Diptera: Tephritidae)

Ono

Hee

Jiang

2021

Insects

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Dacini fruit flies mainly contain two genera, Bactrocera and Zeugodacus, and include many important pests of fruits and vegetables. Their life cycle is affected by various environmental cues. Among them, multiple characteristic semiochemicals have remarkable effects on their reproductive and host-finding behaviors. Notably, floral fragrances released from so-called fruit fly orchids strongly attract males of several Dacini fruit fly species. Focusing on the strong attraction of male flies to particular chemicals, natural and synthetic lures have been used for pest management. Thus, the perception of semiochemicals is important to understand environmental adaptation in Dacini fruit flies. Since next-generation sequencers are available, a large number of chemosensory-related genes have been identified in Dacini fruit flies, as well as other insects. Furthermore, recent studies have succeeded in the functional analyses of olfactory receptors in response to semiochemicals. Thus, characterization of molecular components required for chemoreception is under way. However, the mechanisms underlying chemoreception remain largely unknown. This paper reviews recent findings on peripheral mechanisms in the perception of odors in Dacini fruit flies, describing related studies in other dipteran species, mainly the model insect Drosophilamelanogaster. Based on the review, important themes for future research have also been discussed.

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CRISPR/Cas9 mediated gene knockout reveals a more important role of PBP1 than PBP2 in the perception of female sex pheromone components in Spodoptera litura

Cited by 51 publications

References 47 publications

Transcriptome Characterization and Expression Analysis of Chemosensory Genes in Chilo sacchariphagus (Lepidoptera Crambidae), a Key Pest of Sugarcane

Transcriptome Characterization and Expression Analysis of Chemosensory Genes in Chilo sacchariphagus (Lepidoptera Crambidae), a Key Pest of Sugarcane

Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

Recent Advancements in Studies on Chemosensory Mechanisms Underlying Detection of Semiochemicals in Dacini Fruit Flies of Economic Importance (Diptera: Tephritidae)

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