CRISPR/Cas9-mediated genome editing in Hevea brasiliensis

Dai, Xuemei; Yang, Xianfeng; Chün, Wang; Fan, Yueting; Xin, Shichao; Yue, Hua; Wang, Kejian; Hong, Huang

doi:10.1016/j.indcrop.2021.113418

Cited by 29 publications

(13 citation statements)

References 58 publications

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“…In the case of gRNA1, three lines were heterozygous and one chimeric; in the case of gRNA2, two lines were chimeric and two heterozygous mutants. Our results are in contrast with what was observed in other woody plants (e.g., grape, pear, apple, or poplar) showing a high level of homologous and biallelic mutants in their T 0 generation (Dai et al, 2021 ).…”

Section: Resultscontrasting

confidence: 99%

First Report of CRISPR/Cas9 Gene Editing in Castanea sativa Mill

et al. 2021

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CRISPR/Cas9 has emerged as the most important tool for genome engineering due to its simplicity, design flexibility, and high efficiency. This technology makes it possible to induce point mutations in one or some target sequences simultaneously, as well as to introduce new genetic variants by homology-directed recombination. However, this approach remains largely unexplored in forest species. In this study, we reported the first example of CRISPR/Cas9-mediated gene editing in Castanea genus. As a proof of concept, we targeted the gene encoding phytoene desaturase (pds), whose mutation disrupts chlorophyll biosynthesis allowing for the visual assessment of knockout efficiency. Globular and early torpedo-stage somatic embryos of Castanea sativa (European chestnut) were cocultured for 5 days with a CRISPR/Cas9 construct targeting two conserved gene regions of pds and subsequently cultured on a selection medium with kanamycin. After 8 weeks of subculture on selection medium, four kanamycin-resistant embryogenetic lines were isolated. Genotyping of these lines through target Sanger sequencing of amplicons revealed successful gene editing. Cotyledonary somatic embryos were maturated on maltose 3% and cold-stored at 4°C for 2 months. Subsequently, embryos were subjected to the germination process to produce albino plants. This study opens the way to the use of the CRISPR/Cas9 system in European chestnut for biotechnological applications

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Section: Resultscontrasting

confidence: 99%

First Report of CRISPR/Cas9 Gene Editing in Castanea sativa Mill

et al. 2021

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“…(Glyma.18G003900) (Du et al, 2016) Tragopogon (Shan et al, 2018) Medicago (Meng et al, 2017;Wolabu et al, 2020) Potato (Wang et al, 2015;Bánfalvi et al, 2020) Hevea (Dai et al, 2021) Wheat (Upadhyay et al, 2013;Howells et al, 2018) Lilium (Yan et al, 2019) Kiwifruit (Arabidopsis) (Chen et al, 2010) t20-2, t20-3 (Os12g21710; Rice) (Liu et al, 2020a) htd12 (EMS), t20 (Co-60 radiation; Os12g21710; Rice) (Liu et al, 2020a;Zhou et al, 2021) zic1-1, zic 1-2, ziso-155 (At1g10830)…”

Section: Pds (Genbank Accession Jq762260)mentioning

confidence: 99%

The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants

Gupta

Hirschberg

2022

Front. Plant Sci.

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Carotenoids comprise the most widely distributed natural pigments. In plants, they play indispensable roles in photosynthesis, furnish colors to flowers and fruit and serve as precursor molecules for the synthesis of apocarotenoids, including aroma and scent, phytohormones and other signaling molecules. Dietary carotenoids are vital to human health as a source of provitamin A and antioxidants. Hence, the enormous interest in carotenoids of crop plants. Over the past three decades, the carotenoid biosynthesis pathway has been mainly deciphered due to the characterization of natural and induced mutations that impair this process. Over the year, numerous mutations have been studied in dozens of plant species. Their phenotypes have significantly expanded our understanding of the biochemical and molecular processes underlying carotenoid accumulation in crops. Several of them were employed in the breeding of crops with higher nutritional value. This compendium of all known random and targeted mutants available in the carotenoid metabolic pathway in plants provides a valuable resource for future research on carotenoid biosynthesis in plant species.

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“…The proof of concept for the CRISPR/Cas9 application has been established in several fruit tree species such as citrus [112,113], apple [114,115], grape [116], coffee [117], kiwifruit [118], cacao [119], pomegranate [120], walnut [121], and pear [115]. However, CRISPR-mediated genome editing in forest trees has been mainly achieved in poplar [122], and, for the last three years, in the tropical tree Parasponia andersonii [123], Eucalypts [124], rubber tree [125,126], Monterey pine [127], and European chestnut [128]. For evaluating CRISPR in new tree study systems, several types of the engineered SpCas9 gene sequences with nuclear localization signals and designed with codon optimization for human (hSpCas9, Addgene #42230 [11]), for Arabidopsis thaliana (aSpCas9, Addgene #61433, [129]), for rice (oSpCas9, Addgene #53064 [130]), for grasses including higher GC content at the 5 terminal region (gSpCas9, Addgene #106331 [131]), or even the original coding sequence from Streptococcus pyogenes have been successfully used.…”

Section: Crispr-mediated Genome Editing Provides a Powerful Tool For ...mentioning

confidence: 99%

“…Although Arabidopsis Pol III promoters and the CaMV35S promoter have been widely used to produce sgRNAs and Cas nucleases, respectively, for successful genome editing in most of the reported tree species (Figure 2, Supplemental Table S2), the initial attempt employing Arabidopsis and cotton U6 promoters for driving sgRNA transcription had failed to detect any edited plant in the rubber tree (Hevea brasiliensis). The CRISPR/Cas9 system could finally be established in Hevea brasiliensis by using any of five endogenous U6 promoters with a range of editing efficiencies from 8.47% to 24.92% [125]. Besides, directly compared with Arabidopsis promoters, species-specific U6 promoters were much more efficient for driving sgRNA expression and enhancing the editing efficiency of CRISPR/Cas9 systems in cotton [132] and soybean [133].…”

Section: Crispr-mediated Genome Editing Provides a Powerful Tool For ...mentioning

confidence: 99%

From Genome Sequencing to CRISPR-Based Genome Editing for Climate-Resilient Forest Trees

Cao

Gailing

2022

IJMS

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Due to the economic and ecological importance of forest trees, modern breeding and genetic manipulation of forest trees have become increasingly prevalent. The CRISPR-based technology provides a versatile, powerful, and widely accepted tool for analyzing gene function and precise genetic modification in virtually any species but remains largely unexplored in forest species. Rapidly accumulating genetic and genomic resources for forest trees enabled the identification of numerous genes and biological processes that are associated with important traits such as wood quality, drought, or pest resistance, facilitating the selection of suitable gene editing targets. Here, we introduce and discuss the latest progress, opportunities, and challenges of genome sequencing and editing for improving forest sustainability.

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CRISPR/Cas9-mediated genome editing in Hevea brasiliensis

Cited by 29 publications

References 58 publications

First Report of CRISPR/Cas9 Gene Editing in Castanea sativa Mill

First Report of CRISPR/Cas9 Gene Editing in Castanea sativa Mill

The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants

From Genome Sequencing to CRISPR-Based Genome Editing for Climate-Resilient Forest Trees

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