CRISPR/Cas9‐mediated knockout and overexpression studies reveal a role of maize phytochrome C in regulating flowering time and plant height

Li, Quanquan; Wu, Guangxia; Zhao, Yongping; Wang, Baobao; Zhao, Binbin; Kong, Dexin; Wei, Hongbin; Chen, Cuixia; Wang, Haiyang

doi:10.1111/pbi.13429

Cited by 62 publications

(37 citation statements)

References 59 publications

(105 reference statements)

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“…Moreover, it was shown that GmCRY1s play important roles in mediating LBL-induced SAS through modulating GA metabolism (Lyu et al 2020). Other studies in maize reported that the Zmphyb1 Zmphyb2 double mutant displays constitutive shade avoidance responses, while Zmphyc1 Zmphyc2 double mutant shows moderately early flowering under long-day conditions (Kebrom et al 2006;Li et al 2020;Sheehan et al 2007). Furthermore, expression of these two hyperactive mutant forms of ZmPHYB1 or overexpression of ZmPHYCs effectively reduces the plant height and ear height of mature maize plants in field conditions (Li et al 2020;Zhao et al 2020).…”

Section: Perspective and Conclusionmentioning

confidence: 99%

Integration of light and hormone signaling pathways in the regulation of plant shade avoidance syndrome

2021

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As sessile organisms, plants are unable to move or escape from their neighboring competitors under high-density planting conditions. Instead, they have evolved the ability to sense changes in light quantity and quality (such as a reduction in photoactive radiation and drop in red/far-red light ratios) and evoke a suite of adaptative responses (such as stem elongation, reduced branching, hyponastic leaf orientation, early flowering and accelerated senescence) collectively termed shade avoidance syndrome (SAS). Over the past few decades, much progress has been made in identifying the various photoreceptor systems and light signaling components implicated in regulating SAS, and in elucidating the underlying molecular mechanisms, based on extensive molecular genetic studies with the model dicotyledonous plant Arabidopsis thaliana. Moreover, an emerging synthesis of the field is that light signaling integrates with the signaling pathways of various phytohormones to coordinately regulate different aspects of SAS. In this review, we present a brief summary of the various cross-talks between light and hormone signaling in regulating SAS. We also present a perspective of manipulating SAS to tailor crop architecture for breeding high-density tolerant crop cultivars.

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Section: Perspective and Conclusionmentioning

confidence: 99%

Integration of light and hormone signaling pathways in the regulation of plant shade avoidance syndrome

2021

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“…Tissue culture has been widely used to advance plant research by transgenesis and gene‐editing technologies (Azeez and Busov, 2021 ; Li et al ., 2020 ; Liu et al ., 2019 ). Although successful regeneration of transformed seedlings has been reported in some species, it is still the bottleneck for the C .…”

Section: Discussionmentioning

confidence: 99%

Establishment of anAgrobacterium‐mediated genetic transformation and CRISPR/Cas9‐mediated targeted mutagenesis in Hemp (Cannabis SativaL.)

Zhang

Cheng

et al. 2021

Plant Biotechnology Journal

101

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Hemp (Cannabis sativa L.) is an annual and typically dioecious crop. Due to the therapeutic potential for human diseases, phytocannabinoids as a medical therapy is getting more attention recently. Several candidate genes involved in cannabinoid biosynthesis have been elucidated using omics analysis. However, the gene function was not fully validated due to few reports of stable transformation for Cannabis tissues. In this study, we firstly report the successful generation of gene-edited plants using an Agrobacterium-mediated transformation method in C. sativa. DMG278 achieved the highest shoot induction rate, which was selected as the model strain for transformation. By overexpressing the cannabis developmental regulator chimera in the embryo hypocotyls of immature grains, the shoot regeneration efficiency was substantially increased. We used CRISPR/Cas9 technology to edit the phytoene desaturase gene and finally generated four edited cannabis seedlings with albino phenotype. Moreover, we propagated the transgenic plants and validated the stable integration of T-DNA in cannabis genome.

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“…In rice, it plays an important role in FR-mediated repression of flowering under long-day conditions [ 60 ]. In maize, it has been reported to regulate early flowering and plant height [ 61 ]. Thus, phytochrome C could be a plausible candidate gene for the control of stem elongation and flowering.…”

Section: Discussionmentioning

confidence: 99%

QTL Analysis of Stem Elongation and Flowering Time in Lettuce Using Genotyping-by-Sequencing

Lee

Fukushima

Park

et al. 2021

Genes

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Lettuce plants tend to undergo floral initiation by elongation of flower stalks (bolting) under high-temperature and long-day conditions, which is a serious problem for summer lettuce production. Our objective was to generate a high-density genetic map using SNPs obtained from genotyping-by-sequencing (GBS) analysis of F5 recombinant inbred lines (RILs) and to map QTLs involved in stem growth and flowering time in lettuce. A set of 127 intra-specific RIL mapping populations derived from a cross between two varieties, green and red leaf lettuce, were used to identify QTLs related to the number of days from sowing to bolting (DTB), to flowering of the first flower (DTF), to seed-setting of the first flower (DTS), and the total number of leaves (LN), plant height (PH), and total number of branches of main inflorescence (BN) for two consecutive years. Of the 15 QTLs detected, one that controls DTB, DTF, DTS, LN, and PH detected on LG 7, and another QTL that controls DTF, DTS, and PH detected on LG 1. Analysis of the genomic sequence corresponding to the QTL detected on LG 7 led to the identification of 22 putative candidate genes. A consistent QTL related to bolting and flowering time, and corresponding candidate genes has been reported. This study will be valuable in revealing the genetic basis of stem growth and flowering time in lettuce.

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CRISPR/Cas9‐mediated knockout and overexpression studies reveal a role of maize phytochrome C in regulating flowering time and plant height

Cited by 62 publications

References 59 publications

Integration of light and hormone signaling pathways in the regulation of plant shade avoidance syndrome

Integration of light and hormone signaling pathways in the regulation of plant shade avoidance syndrome

Establishment of anAgrobacterium‐mediated genetic transformation and CRISPR/Cas9‐mediated targeted mutagenesis in Hemp (Cannabis SativaL.)

QTL Analysis of Stem Elongation and Flowering Time in Lettuce Using Genotyping-by-Sequencing

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