CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

Noureen, Azka; Khan, Muhammad Zuhaib; Amin, Imran; Zainab, Tayyaba; Mansoor, Shahid

doi:10.3389/fgene.2022.922019

Cited by 15 publications

(3 citation statements)

References 41 publications

(47 reference statements)

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“…RNA viruses hijack host cellular machinery, including eIF4E, eIF4G, and their isoforms, to complete their life cycle (Sanfacon, 2015). Different isoforms of eIF4E were targeted by CRISPR/Cas9 to generate resistant varieties in tomato, potato, and cucumber against viruses belonging to Potyviridae (Chandrasekaran et al, 2016;Atarashi et al, 2020;Yoon et al, 2020;Lucioli et al, 2022;Noureen et al, 2022).…”

Section: Crispr/cas Mediated Resistance Against Virusesmentioning

confidence: 99%

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Sardar

2023

Front. Plant Sci.

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Environmental changes and increasing population are major concerns for crop production and food security as a whole. To address this, researchers had focussed on the improvement of cereals and pulses and have made considerable progress till the beginning of this decade. However, cereals and pulses together, without vegetables and fruits, are inadequate to meet the dietary and nutritional demands of human life. Production of good quality vegetables and fruits is highly challenging owing to their perishable nature and short shelf life as well as abiotic and biotic stresses encountered during pre- and post-harvest. Genetic engineering approaches to produce good quality, to increase shelf life and stress-resistance, and to change the time of flowering and fruit ripening by introducing foreign genes to produce genetically modified crops were quite successful. However, several biosafety concerns, such as the risk of transgene-outcrossing, limited their production, marketing, and consumption. Modern genome editing techniques, like the CRISPR/Cas9 system, provide a perfect solution in this scenario, as it can produce transgene-free genetically edited plants. Hence, these genetically edited plants can easily satisfy the biosafety norms for crop production and consumption. This review highlights the potential of the CRISPR/Cas9 system for the successful generation of abiotic and biotic stress resistance and thereby improving the quality, yield, and overall productivity of vegetables and fruits.

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Section: Crispr/cas Mediated Resistance Against Virusesmentioning

confidence: 99%

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Sardar

2023

Front. Plant Sci.

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“…They found that only the TaeIF4E triple mutant was completely resistant to WYMV and set fruit normally; the single or double mutant remained susceptible and showed dwarf plants and severely reduced fruit set after susceptibility [ 66 ]. Not only has multi-gene editing been used on a large scale in major crops, but new superior traits have been created through multi-gene editing in a variety of crops such as tomatoes, apples, bananas, and potatoes ( Solanum tuberosum L.) [ 67 , 68 , 69 , 70 , 71 , 72 ].…”

Section: Advances In Crispr/cas9 Gene Editing Technology In the Field...mentioning

confidence: 99%

Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding

Zhou

Luan

Liu

et al. 2023

Plants

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Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has become increasingly mature and is being widely used for crop improvement. The application of CRISPR/Cas9 enables the generation of transgene-free genome-edited plants in a short period and has the advantages of simplicity, high efficiency, high specificity, and low production costs, which greatly facilitate the study of gene functions. In plant molecular breeding, the gene-editing efficiency of the CRISPR-Cas9 system has proven to be a key step in influencing the effectiveness of molecular breeding, with improvements in gene-editing efficiency recently becoming a focus of reported scientific research. This review details strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding, including Cas9 variant enzyme engineering, the effect of multiple promoter driven Cas9, and gRNA efficient optimization and expression strategies. It also briefly introduces the optimization strategies of the CRISPR/Cas12a system and the application of BE and PE precision editing. These strategies are beneficial for the further development and optimization of gene editing systems in the field of plant molecular breeding.

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“…RNA viruses need plant host factors to preserve their normal life cycle, containing the eukaryotic translation initiation factors eIF4E , eIF4G , and eIF(iso)4E [ 138 ]. Host susceptibility gene eIF4E was targeted at two different sites to create resistance against plant potyviruses by CRISPR/Cas9 [ 52 , 98 , 99 ]. A similar approach in A. thaliana plants induced site-specific mutations at eIF(iso)4E locus and conferred complete resistance to single-stranded RNA potyvirus -TuMV by 1 bp deletions and 1 bp insertions without any off-target modification [ 95 ].…”

Section: Crispr/cas Technique For Disease Resistancementioning

confidence: 99%

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Wang

Zafar

Ali

et al. 2022

Cells

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Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game-changing tool that can create variation in desired traits, such as biotic and abiotic resistance, increase quality and yield in less time with easy applications, high efficiency, and low cost in producing the targeted edits for rapid improvement of crop plants. Plant pathogens and the severe environment cause considerable crop losses worldwide. GE approaches have emerged and opened new doors for breeding multiple-resistance crop varieties. Here, we have summarized recent advances in CRISPR/Cas-mediated GE for resistance against biotic and abiotic stresses in a crop molecular breeding program that includes the modification and improvement of genes response to biotic stresses induced by fungus, virus, and bacterial pathogens. We also discussed in depth the application of CRISPR/Cas for abiotic stresses (herbicide, drought, heat, and cold) in plants. In addition, we discussed the limitations and future challenges faced by breeders using GE tools for crop improvement and suggested directions for future improvements in GE for agricultural applications, providing novel ideas to create super cultivars with broad resistance to biotic and abiotic stress.

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CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

Cited by 15 publications

References 41 publications

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing

Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

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