CRISPR-Cas9: Role in Processing of Modular Metabolic Engineered Bio-Based Products

Abstract

Biogenetic engineering is a significant technology to sensibly manage microbial metabolic product factories. Genome modification methods for efficiently controlling and modifying genes at the genome level have progressed in biogenetic engineering during the last decade. CRISPR is genome editing technology that allows for the modification of organisms’ genomes. CRISPR and its related RNA-guided endonuclease are versatile advanced immune system frameworks for defending against foreign DNA and RNAs. CRISPR is efficient, accessible, and trustworthy genomic modification tool in unparalleled resolution. At present, CRISPR-Cas9 method is expanded to industrially manipulate cells. Metabolically modified organisms are quickly becoming interested in the production of different bio-based components. Here, chapter explore about the control productivity of targeted biomolecules in divergent cells based on the use of different CRISPR-related Cas9.

Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

“…The combination of PCR techniques, next-generation sequencing, directed or adaptive laboratory evolution approaches, and the development of robust recombinant gene expression hosts and, more recently, the breakthrough gene-editing technique CRISPR-Cas9 (clustered regularly interspaced short palindromic repeat-associated nuclease 9) have accelerated the study and application of laccases. [101][102][103][104][105][106][107] The merging of nanotechnology and recombinant DNA approaches has provided successful examples of the development of efficient laccase removal of lignin inhibitors with easy recovery and reuse of the enzyme. 108 Laccase immobilization and functionalization of various materials have been described in the literature for the removal of toxic compounds, as native or recombinant laccases can be grafted onto the surface of nanomaterials to enhance storage stability and reusability.…”

“…The use of culture‐independent techniques has broadened the search for laccases from environmental samples, and novel laccases have been screened in a culture‐independent manner and isolated by gene mining from databases and functional metagenomics studies, for example, GenBank, biocatnet.de, http://metacyc.org. The combination of PCR techniques, next‐generation sequencing, directed or adaptive laboratory evolution approaches, and the development of robust recombinant gene expression hosts and, more recently, the breakthrough gene‐editing technique CRISPR‐Cas9 (clustered regularly interspaced short palindromic repeat‐associated nuclease 9) have accelerated the study and application of laccases 101–107 …”