CRISPR-Cas9 system for fungi genome engineering toward industrial applications

Satish, Lakkakula; Shamili, Sasanala; Muthubharathi, Balasubramanian Chellammal; Ceasar, Stanislaus Antony; Kushmaro, Ariel; Singh, Vijai; Sitrit, Yaron

doi:10.1016/b978-0-12-818140-9.00006-4

Cited by 7 publications

(5 citation statements)

References 86 publications

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“…CRISPR/Cas9 technology has been successfully developed in fungi for genome editing such as Trichoderma reesei, Aspergillus stains, A. fumigatus, and Ustilago maydis (see Table 4) [29][30][31]. For example, genome editing of multiple genes was successfully achieved in T. reesei through CRISPR/Cas9 technology by following the co-transformation via in vitro synthesis of sgRNAs and donor DNA with the 200 bp homologous arms.…”

Section: Role Of Crispr Technology In Fungal Enzymesmentioning

confidence: 99%

“…For instance, a selected gene that encodes target enzyme shows its expression in the fungal cell factory, such as T. reesei, A. nidulans and A. oryzae, A. oryzae FG76, Pseudomonas aeruginosa CTM50182 and P. chrysogenum (Pg222) [24][25][26][27][28]. The accumulative data also revealed that CRISPR/Cas9 technology had been successfully developed in different fungi, including Trichoderma reesei, Aspergillus stains, A. fumigatus, and Ustilago maydis for genome editing [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

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Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review

Naeem

Manzoor²,

Abid

et al. 2022

JoF

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With the increasing world population, demand for industrialization has also increased to fulfill humans’ living standards. Fungi are considered a source of essential constituents to produce the biocatalytic enzymes, including amylases, proteases, lipases, and cellulases that contain broad-spectrum industrial and emerging applications. The present review discussed the origin, nature, mechanism of action, emerging aspects of genetic engineering for designing novel proteases, genome editing of fungal strains through CRISPR technology, present challenges and future recommendations of fungal proteases. The emerging evidence revealed that fungal proteases show a protective role to many environmental exposures and discovered that an imbalance of protease inhibitors and proteases in the epithelial barriers leads to the protection of chronic eosinophilic airway inflammation. Moreover, mitoproteases recently were found to execute intense proteolytic processes that are crucial for mitochondrial integrity and homeostasis function, including mitochondrial biogenesis, protein synthesis, and apoptosis. The emerging evidence revealed that CRISPR/Cas9 technology had been successfully developed in various filamentous fungi and higher fungi for editing of specific genes. In addition to medical importance, fungal proteases are extensively used in different industries such as foods to prepare butter, fruits, juices, and cheese, and to increase their shelf life. It is concluded that hydrolysis of proteins in industries is one of the most significant applications of fungal enzymes that led to massive usage of proteomics.

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Section: Role Of Crispr Technology In Fungal Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review

Naeem

Manzoor²,

Abid

et al. 2022

JoF

View full text Add to dashboard Cite

show abstract

“…Once primarily the domain of model organisms, genetic tractability has been democratized by CRISPR-cas9 methods (Satish et al, 2020 ). An attractive target for future genetic engineering method development includes anaerobic fungi who produce scaffolded plant biomass degrading enzyme complexes called cellulosomes (Hooker et al, 2019 ; Wilken et al, 2020 ).…”

Section: Synthetic and Systems Biologymentioning

confidence: 99%

Grand Challenges in Fungal Biotechnology

Baker

2020

Front. Fungal Biol.

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“…The CRISPR-Cas system is an effective tool that can be applied to replacing, deleting, or inserting genes into the genomes of both eukaryotic and prokaryotic organisms ( Jinek et al, 2012 ; Cong et al, 2013 ; Qi et al, 2013 ; Hsu et al, 2014 ). Crisper-Cas technology has been used to edit the genomes of several filamentous fungi; however, there is a limited study on endophytic fungi genome editing ( Salazar-Cerezo et al, 2020 ; Satish et al, 2020 ). Although endophytic fungi produce many bioactive compounds, and CRISPR/Cas9 technology may increase their bioactive compound production, there are no review articles available on this topic.…”

Section: Introductionmentioning

confidence: 99%

Bioengineering of fungal endophytes through the CRISPR/Cas9 system

et al. 2023

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The CRISPR/Cas9 system is a genome-editing tool that allows for precise and efficient modifications to the DNA of a cell. This technology can be used in endophytic fungi, which live within plants and can have beneficial effects on their host, making them important for agriculture. Using CRISPR/Cas9, researchers can introduce specific genetic changes into endophytic fungal genomes, allowing them to study the function of genes, improve their plant-growth-promoting properties, and create new, more beneficial endophytes. This system works by using the Cas9 protein, which acts as a pair of molecular scissors, to cut DNA at specific locations determined by a guide RNA. Once the DNA is cut, the cell’s natural repair mechanisms can be used to insert or delete specific genes, allowing for precise editing of the fungal genome. This article discusses the mechanism and applications of CRISPR/Cas9 to fungal endophytes.

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CRISPR-Cas9 system for fungi genome engineering toward industrial applications

Cited by 7 publications

References 86 publications

Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review

Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review

Grand Challenges in Fungal Biotechnology

Bioengineering of fungal endophytes through the CRISPR/Cas9 system

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