CRISPR/Cas9-targeted mutagenesis of OsERA1 confers enhanced responses to abscisic acid and drought stress and increased primary root growth under nonstressed conditions in rice

Ogata, Tatsushi; Ishizaki, Takuma; Fujita, Miki; Fujita, Yasunari

doi:10.1371/journal.pone.0243376

Cited by 101 publications

(31 citation statements)

References 45 publications

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“…These mutant lines showed improved main root development compared to WT; through stomatal regulation, these osera1 mutant lines also had better sensitivity to ABA and drought stress. These results indicate that OsERA1 in rice can serve as a negative regulator of primary root development in non-stressed circumstances as well as a negative regulator of ABA and drought stress responses [100].…”

Section: Drought Tolerancementioning

confidence: 79%

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Kim

Jung

et al. 2022

Agronomy

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Nowadays, rice production faces significant challenges due to population pressure, global climate change, and outbreak of various pests and diseases. Breeding techniques used to improve rice traits include mutant breeding, cross breeding, heterogeneity, transformation, molecular markers, genome-wide association study (GWAS), and so on. Since the recently developed CRISPR/Cas9 technology can directly target a specific part of a desired gene to induce mutation, it can be used as a powerful means to expand genetic diversity of crops and develop new varieties. So far, CRISPR/Cas9 technology has been used for improving rice characteristics such as high yield, good quality, abundant nutrition, pest and disease resistance, herbicide resistance, and biotic and abiotic stress resistance. This review highlights the mechanisms and optimization of the CRISPR system and its application to rice crop, including resistance to biotic and abiotic stresses, and improved rice quality and yield.

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Section: Drought Tolerancementioning

confidence: 79%

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Kim

Jung

et al. 2022

Agronomy

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“…The relative stomatal conductance rates of the mutant plants were significantly lower than those of the wild-type plants. The Osera1 mutant exhibits increased sensitivity to ABA [ 62 ]. Leaf morphology affects plant tolerance to drought, and genes for the curled leaf phenotype by controlling the quantity, size, and arrangement of bulliform cells (BCs) were influenced.…”

Section: Abiotic Stress Responses Revealed By Genome Editingmentioning

confidence: 99%

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Fuhrmann-Aoyagi

et al. 2022

CIMB

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Global warming and climate change have severely affected plant growth and food production. Therefore, minimizing these effects is required for sustainable crop yields. Understanding the molecular mechanisms in response to abiotic stresses and improving agricultural traits to make crops tolerant to abiotic stresses have been going on unceasingly. To generate desirable varieties of crops, traditional and molecular breeding techniques have been tried, but both approaches are time-consuming. Clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALENs) are genome-editing technologies that have recently attracted the attention of plant breeders for genetic modification. These technologies are powerful tools in the basic and applied sciences for understanding gene function, as well as in the field of crop breeding. In this review, we focus on the application of genome-editing systems in plants to understand gene function in response to abiotic stresses and to improve tolerance to abiotic stresses, such as temperature, drought, and salinity stresses.

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“…In another study, the Enhanced response to ABA1 (ERA1) gene present in Arabidopsis thaliana was targeted, that encode the farnesyltransferase and upregulate the abscisic acid (ABA) signaling under heat and drought stress. By using CRISPR/Cas9 system, they knock in the ERA1 gene into rice genome and enhanced the ABA in rice under heat and water deficit condition (Ogata et al, 2020 ).…”

Section: Plant Heat-adaptation Strategiesmentioning

confidence: 99%

What Did We Learn From Current Progress in Heat Stress Tolerance in Plants? Can Microbes Be a Solution?

et al. 2022

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Temperature is a significant parameter in agriculture since it controls seed germination and plant growth. Global warming has resulted in an irregular rise in temperature posing a serious threat to the agricultural production around the world. A slight increase in temperature acts as stress and exert an overall negative impact on different developmental stages including plant phenology, development, cellular activities, gene expression, anatomical features, the functional and structural orientation of leaves, twigs, roots, and shoots. These impacts ultimately decrease the biomass, affect reproductive process, decrease flowering and fruiting and significant yield losses. Plants have inherent mechanisms to cope with different stressors including heat which may vary depending upon the type of plant species, duration and degree of the heat stress. Plants initially adapt avoidance and then tolerance strategies to combat heat stress. The tolerance pathway involves ion transporter, osmoprotectants, antioxidants, heat shock protein which help the plants to survive under heat stress. To develop heat-tolerant plants using above-mentioned strategies requires a lot of time, expertise, and resources. On contrary, plant growth-promoting rhizobacteria (PGPRs) is a cost-effective, time-saving, and user-friendly approach to support and enhance agricultural production under a range of environmental conditions including stresses. PGPR produce and regulate various phytohormones, enzymes, and metabolites that help plant to maintain growth under heat stress. They form biofilm, decrease abscisic acid, stimulate root development, enhance heat shock proteins, deamination of ACC enzyme, and nutrient availability especially nitrogen and phosphorous. Despite extensive work done on plant heat stress tolerance in general, very few comprehensive reviews are available on the subject especially the role of microbes for plant heat tolerance. This article reviews the current studies on the retaliation, adaptation, and tolerance to heat stress at the cellular, organellar, and whole plant levels, explains different approaches, and sheds light on how microbes can help to induce heat stress tolerance in plants.

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CRISPR/Cas9-targeted mutagenesis of OsERA1 confers enhanced responses to abscisic acid and drought stress and increased primary root growth under nonstressed conditions in rice

Cited by 101 publications

References 45 publications

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

What Did We Learn From Current Progress in Heat Stress Tolerance in Plants? Can Microbes Be a Solution?

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