CRISPR/Cas9 Technology and Its Utility for Crop Improvement

Liu, Hua; Chen, Wendan; Li, Yushu; Sun, Lina; Chai, Yuhong; Chen, Hạixia; Nie, Haochen; Huang, Conglin

doi:10.3390/ijms231810442

Cited by 27 publications

(11 citation statements)

References 138 publications

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“…Field exams have further confirmed the potential of CRISPR/Cas-mediated genome editing to reinforce agricultural productivity. For instance, transgenic maize with the AGROS8 gene edited by means of CRISPR/Cas exhibited a sizable boom in grain yield, especially below drought situations [23]. Similarly, CRISPR/Cas9-edited versions of OsLOGL5 in rice constantly improved grain manufacturing across extraordinary environmental situations, consisting of drought and coffee nitrogen remedies.…”

Section: Utilising Crispr/cas To Enhance Agricultural Yieldmentioning

confidence: 99%

Role of CRISPR in Crop Improvement: A Review

Raza,

Abdullah,

Khaliq

et al. 2024

AJRAF

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The meals and agriculture sectors have witnessed good sized advancements due to the state-of-the-art improvements in agricultural biotechnology and genetic engineering, that have more advantageous the essential characteristics of plant agronomic tendencies. A extensively used technique for inducing focused deletions, insertions, and precise sequence adjustments throughout numerous species and mobile kinds is collection-particular nucleases (SSNs)-based centered genome editing. Commercial adoption of genome modifying gear, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and siRNA-mediated RNA interference, has been significant. However, the whole landscape of lifestyles sciences underwent a paradigm shift with the invention of the CRISPR/Cas9 machine as a flexible tool for genome modifying. Initially recognized as a virological defense DNA segment in bacteria and archaea, the clustered Regularly interspaced short palindromic repeats (CRISPR) machine has revolutionized molecular biology. Through modern molecular organic strategies, CRISPR/Cas9 enables unique modifications in any crop species. Its efficacy, reproducibility, and specificity have earned CRISPR/Cas9 the moniker of a "leap forward" in biotechnology.

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Section: Utilising Crispr/cas To Enhance Agricultural Yieldmentioning

confidence: 99%

Role of CRISPR in Crop Improvement: A Review

Raza,

Abdullah,

Khaliq

et al. 2024

AJRAF

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“…From the perspective of biosafety, it is necessary to obtain lines from which foreign genes have been removed by the genetic separation of sexual generations. However, this is impossible (Liu et al 2022). To avoid importing foreign genes, RNP transfection methods by particle bombardment or polyethylene glycol (PEG)-Ca 2+ mediation have been developed in the genome editing of plants (Liang et al 2017, Toda et al 2019, 2023.…”

Section: New Genome Editing Factor St8mentioning

confidence: 99%

“…Among the various CRISPR systems, Cas9 has been the most widely used and is considered the most advanced in terms of its applications. An abundance of reports have already been published on the use of the CRISPR-Cas9 system to produce genome-edited grains, vegetables, and fruit, among others (Ahmad 2023, Devi et al 2022, Gan and Ling 2022, Guo et al 2023, Liu et al 2022, Wang et al 2022. Consequently, this review is centered on CRISPR-Cas12a, a unique effector distinct from CRISPR-Cas9; CRISPR-MAD7, a Cas12a variant originating from Eubacterium rectale; and ST8, an improved variant of MAD7.…”

Section: Introductionmentioning

confidence: 99%

Cas12a and MAD7, genome editing tools for breeding

Hozumi,

Chen,

Takemoto

et al. 2024

Breed. Sci.

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Food shortages due to population growth and climate change are expected to occur in the near future as a problem that urgently requires solutions. Conventional breeding techniques, notably crossbreeding and mutation breeding, are known for being inefficient and time-consuming in obtaining seeds and seedlings with desired traits. Thus, there is an urgent need for novel methods for efficient plant breeding. Breeding by genome editing is receiving substantial attention because it can efficiently modify the target gene to obtain desired traits compared with conventional methods. Among the programmable sequence-specific nucleases that have been developed for genome editing, CRISPR-Cas12a and CRISPR-MAD7 nucleases are becoming more broadly adopted for the application of genome editing in grains, vegetables and fruits. Additionally, ST8, an improved variant of MAD7, has been developed to enhance genome editing efficiency and has potential for application to breeding of crops.

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“…), tomato ( Solanum lycopersicum L. ), soybean ( Glycine max (L.) Merr. ), and rice ( Figure 1 ) [ 54 , 55 , 56 ]. Its intended applications include increasing crop yields, improving crop quality traits, enhancing resistance to abiotic or biotic stresses, etc.…”

Section: Advances In Crispr/cas9 Gene Editing Technology In the Field...mentioning

confidence: 99%

Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding

Zhou

Luan

Liu

et al. 2023

Plants

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Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has become increasingly mature and is being widely used for crop improvement. The application of CRISPR/Cas9 enables the generation of transgene-free genome-edited plants in a short period and has the advantages of simplicity, high efficiency, high specificity, and low production costs, which greatly facilitate the study of gene functions. In plant molecular breeding, the gene-editing efficiency of the CRISPR-Cas9 system has proven to be a key step in influencing the effectiveness of molecular breeding, with improvements in gene-editing efficiency recently becoming a focus of reported scientific research. This review details strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding, including Cas9 variant enzyme engineering, the effect of multiple promoter driven Cas9, and gRNA efficient optimization and expression strategies. It also briefly introduces the optimization strategies of the CRISPR/Cas12a system and the application of BE and PE precision editing. These strategies are beneficial for the further development and optimization of gene editing systems in the field of plant molecular breeding.

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CRISPR/Cas9 Technology and Its Utility for Crop Improvement

Cited by 27 publications

References 138 publications

Role of CRISPR in Crop Improvement: A Review

Role of CRISPR in Crop Improvement: A Review

Cas12a and MAD7, genome editing tools for breeding

Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding

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