CRISPR-mediated knockdown of miR-214 modulates cell fate in response to anti-cancer drugs in HPV-negative and HPV-positive cervical cancer cells

Sen, Prakriti; Ghosal, Sayam; Hazra, Rudranil; Mohanty, Rimjhim; Arega, Solomon; Sahu, Bikash; Ganguly, Niladri

doi:10.1007/s12038-020-00054-1

Cited by 6 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR-214-3p was a miRNA involved in many cancer, including lung cancer, breast cancer, colon cancer, endometrial carcinoma, and pancreatic cancer in humans [ 31 , 32 ]. In cervical cancer, miR-214-3p has been found play an important roles in regulating cell proliferation, apoptosis, cell invasion, metastasis and angiogenesis [ 33 , 34 ]. Unfortunately, although the potential effectiveness was identified in cervical cancer, the mechanism and status of miR-214-3p involve in ceRNA network in cervical cancer cells needs to be addressed.…”

Section: Introductionmentioning

confidence: 99%

LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells

et al. 2021

View full text Add to dashboard Cite

Background Cervical cancer (CC) is one of the most common gynaecological malignancies all around the world. The mechanisms of cervical carcinoma formation remain under close scrutiny. The long non-coding RNAs (lncRNA) and microRNAs (miRNAs) play important roles in controlling gene expression and promoting the development and progression of cervical cancer by acting as competitive endogenous RNA (ceRNA). However, the roles of lncRNA associated with ceRNAs in cervical carcinogenesis remains unknown. In this study, the expression of long non-coding RNA HOTAIR was investigated in HPV16 positive cervical cancer cells, the candidate miRNAs and target genes were identified to clarify putative ceRNAs of HOTAIR/miRNA in cervical cancer cells. Methods The proliferation ability of cells was measured by CCK8 and EdU incorporation assays and cell apoptosis was analyzed by flow cytometry. The expression of HOTAIR, miR-214-3p, HPV16 E7 mRNA were detected by qRT-PCR. As for searching for the interaction between miR-214-3p and HOTAIR, the binding sites for miR-214-3p on HOTAIR was predicted by starbase v2.0 database, then dual-luciferase assay was used to verify the binding sites. In addition, Gene Ontology (GO) and protein–protein interaction (PPI) network analysis of target genes of miR-214-3p were performed with bioinformatics analysis. The potential signal pathway regulated by HOTAIR/miR-214-3p was predicted by KEGG enrichment analysis and confirmed by qPCR and WB analysis in cervical cancer cells. Results Our results showed that expression of HOTAIR was up-regulated, while that of miR-214-3p was down-regulated in HPV16-positive cervical cancer cells. The expression status of HPV16 E7 played an important role in regulating expression of HOTAIR or miR-214-3p in cervical cancer cells. HOTAIR knockdown could significantly inhibited cell proliferate ability and promote cellular apoptosis, whereas the inhibition of miR-214-3p expression partially reversed such results. Bioinformatics analysis identified 1451 genes as target genes of miR-214-3p. The Gene ontology (GO) and KEGG Pathway enrichment analysis showed that these target genes were mainly related to regulation of cell communication, protein binding, enzyme binding and transferase activity, and Wnt ligand biogenesis. Pathway enrichment analysis results showed that the predicted target genes were significantly enriched in Wnt/β-catenin signaling pathway. Finally, our results confirmed that miR-214-3p could significantly inhibit β-catenin expression in HPV16 positive cancer cells by qPCR and WB analysis. Conclusion HOTAIR could act as a ceRNA through binding to miR-214-3p, promote cell proliferation and inhibit the apoptosis of HPV16 positive cervical cancer. HOTAIR/miR-214-3p/Wnt/β-catenin signal pathway might played important regulated roles in HPV16 positive cervical cancer. Our results provided new insight into defining novel biomarkers for cervical cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The miR-214 was CRISPR knocked out overexpressed in the 3 different cell lines. Cell viability was determined upon treatment with CDPP and a decreased sensitivity of the cells towards the drugs was observed in miR-214 knockout (CP) while miR-214 overexpression showed CDDP sensitivity increase [225]. In a study conducted by Pirouzfar et al [226] used CRISPR/Cas9 technology to target MLL5 along with CDDP to evaluate its effect on the viability and apoptosis of HeLa CC cells apoptosis and viability.…”

Section: Crispr/cas9mentioning

confidence: 99%

Cellular landscaping of cisplatin resistance in cervical cancer

Bhattacharjee

Dey

Kumar

et al. 2022

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

“…Knockdown of Linc00511 could reduce paclitaxel resistance by regulating Bcl-2, MMP-2, MMP-9, MRP1, and P-GP expressions in HeLa cells [ 136 ]. Overexpressed miR-214 under paclitaxel treatment could cause an increase in PARP and a decline in PI-3 kinase/Akt levels [ 137 ]. Circular RNA CircMYBL2 could enhance paclitaxel resistance by upregulating EGFR mediated by microRNA-665 in vitro and promoting tumor growth in vivo [ 138 ].…”

Section: Inferring Heterogeneity With Therapeutic Diversitymentioning

confidence: 99%

Cervical cancer heterogeneity: a constant battle against viruses and drugs

et al. 2022

View full text Add to dashboard Cite

Cervical cancer is the first identified human papillomavirus (HPV) associated cancer and the most promising malignancy to be eliminated. However, the ever-changing virus subtypes and acquired multiple drug resistance continue to induce failure of tumor prevention and treatment. The exploration of cervical cancer heterogeneity is the crucial way to achieve effective prevention and precise treatment. Tumor heterogeneity exists in various aspects including the immune clearance of viruses, tumorigenesis, neoplasm recurrence, metastasis and drug resistance. Tumor development and drug resistance are often driven by potential gene amplification and deletion, not only somatic genomic alterations, but also copy number amplifications, histone modification and DNA methylation. Genomic rearrangements may occur by selection effects from chemotherapy or radiotherapy which exhibits genetic intra-tumor heterogeneity in advanced cervical cancers. The combined application of cervical cancer therapeutic vaccine and immune checkpoint inhibitors has become an effective strategy to address the heterogeneity of treatment. In this review, we will integrate classic and recently updated epidemiological data on vaccination rates, screening rates, incidence and mortality of cervical cancer patients worldwide aiming to understand the current situation of disease prevention and control and identify the direction of urgent efforts. Additionally, we will focus on the tumor environment to summarize the conditions of immune clearance and gene integration after different HPV infections and to explore the genomic factors of tumor heterogeneity. Finally, we will make a thorough inquiry into completed and ongoing phase III clinical trials in cervical cancer and summarize molecular mechanisms of drug resistance among chemotherapy, radiotherapy, biotherapy, and immunotherapy.

show abstract

CRISPR-mediated knockdown of miR-214 modulates cell fate in response to anti-cancer drugs in HPV-negative and HPV-positive cervical cancer cells

Cited by 6 publications

References 43 publications

LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells

LncRNA HOTAIR promotes proliferation and inhibits apoptosis by sponging miR-214-3p in HPV16 positive cervical cancer cells

Cellular landscaping of cisplatin resistance in cervical cancer

Cervical cancer heterogeneity: a constant battle against viruses and drugs

Contact Info

Product

Resources

About