CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases

Xia, Yupiao; Rao, Ruotong; Xiong, Mengqiu; He, Bangshun; Zheng, Bingxin; Jia, Yanwei; Li, Ying; Yang, Yunhuang

doi:10.1021/acs.analchem.3c04363

Cited by 6 publications

(1 citation statement)

References 144 publications

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“…Although the operational mechanisms of CRISPR/Cas biosensors vary depending on the types of Cas effectors and CRISPR/Cas systems, activated CRISPR/Cas can induce signals through cleavage-based and binding-based modes [ 128 , 133 , 134 ]. Thus, various types of CRISPR/Cas-based biosensors can be obtained by integrating targets in existing sensor systems [ 135 , 136 , 137 ]. Depending on the signals from targets, CRISPR/Cas-based sensors employ appropriate signal transducers to detect fluorescent, colorimetric, and electrochemical changes [ 138 ].…”

Section: Biosensor Systems For Cell Monitoringmentioning

confidence: 99%

The Biomedical Applications of Biomolecule Integrated Biosensors for Cell Monitoring

Song,

Hwang,

Jeon

et al. 2024

IJMS

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Cell monitoring is essential for understanding the physiological conditions and cell abnormalities induced by various stimuli, such as stress factors, microbial invasion, and diseases. Currently, various techniques for detecting cell abnormalities and metabolites originating from specific cells are employed to obtain information on cells in terms of human health. Although the states of cells have traditionally been accessed using instrument-based analysis, this has been replaced by various sensor systems equipped with new materials and technologies. Various sensor systems have been developed for monitoring cells by recognizing biological markers such as proteins on cell surfaces, components on plasma membranes, secreted metabolites, and DNA sequences. Sensor systems are classified into subclasses, such as chemical sensors and biosensors, based on the components used to recognize the targets. In this review, we aim to outline the fundamental principles of sensor systems used for monitoring cells, encompassing both biosensors and chemical sensors. Specifically, we focus on biosensing systems in terms of the types of sensing and signal-transducing elements and introduce recent advancements and applications of biosensors. Finally, we address the present challenges in biosensor systems and the prospects that should be considered to enhance biosensor performance. Although this review covers the application of biosensors for monitoring cells, we believe that it can provide valuable insights for researchers and general readers interested in the advancements of biosensing and its further applications in biomedical fields.

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