CRISPR technologies for stem cell engineering and regenerative medicine

Hsu, Mu‐Nung; Chang, Yu‐Han; Truong, Vu Anh; Lai, Po‐Liang; Nguyen, Thị Kieu Nuong; Hu, Yu‐Chen

doi:10.1016/j.biotechadv.2019.107447

Cited by 67 publications

(54 citation statements)

References 215 publications

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“…Class 2 CRISPR-Cas systems, the best-studied system with single effector protein (e.g., Cas9, Cas12, or Cas13) for foreign DNA or RNA interference, are subdivided into Type II (Cas9), Type V (Cas12), and Type VI (Cas13). In the past few years, Class 2 CRISPR-Cas systems have revolutionized both basic and clinical researches, enabling more rapid, precise, and robust genome editing and modifications in cultured cells and animals [8][9][10][11][12][13][14][15][16][17] . However, there were only a few applications of Class 1 CRISPR-Cas (Type I, Type III and Type IV) system.…”

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confidence: 99%

Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells

et al. 2020

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Class 2 CRISPR-Cas proteins have been widely developed as genome editing and transcriptional regulating tools. Class 1 type I CRISPR-Cas constitutes~60% of all the CRISPR-Cas systems. However, only type I-B and I-E systems have been used to control mammalian gene expression and for genome editing. Here we demonstrate the feasibility of using type IF system to regulate human gene expression. By fusing transcription activation domain to Pseudomonas aeruginosa type IF Cas proteins, we activate gene transcription in human cells. In most cases, type IF system is more efficient than other CRISPR-based systems. Transcription activation is enhanced by elongating the crRNA. In addition, we achieve multiplexed gene activation with a crRNA array. Furthermore, type IF system activates target genes specifically without off-target transcription activation. These data demonstrate the robustness and programmability of type IF CRISPR-Cas in human cells.

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confidence: 99%

Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells

et al. 2020

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“…Particularly, removing or adding communication pathways with genome editing and cell engineering technologies 157 will modify cellular phenotypes to control how pairs of cells interact. This approach will have a great impact on many fields, such as developmental biology 158 , wherein CCIs are fundamental for cell differentiation into specific lineages. Biomedicine and biotherapeutics will benefit further from controlling CCIs, particularly in modifying disease courses, as is currently done with immune checkpoint inhibitors 159 , 160 .…”

Section: Challenges and Future Directionsmentioning

confidence: 99%

Deciphering cell–cell interactions and communication from gene expression

et al. 2020

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“…PD-1 has been identified as an important target for the engineering of T-cells [103,104]. CRISPR technologies have been widely applied in stem cells and regenerative medicine [105][106][107]. In recent years, several methods of nanoparticle-mediated CRISPR/Cas9 delivery systems have been well established [108].…”

Section: Applications Of Crispr-cas9 In Biomedical Engineeringmentioning

confidence: 99%

“…In recent years, CRISPR technologies, including CRISPR-mediated genome editing, CRISPRa, and CRISPRi have been widely used in gene expression studies and genome sequence manipulation. Furthermore, these systems have been applied in stem cell engineering and regenerative medicine [105]. Although the effectiveness of the CRISPR-Cas system has been documented, safe and efficient delivery of the CRISPR components to desired cells in patients' needs to be addressed.…”

Section: Perspectives and Future Directionsmentioning

confidence: 99%

An overview of applications of CRISPR-Cas technologies in biomedical engineering

Jamehdor¹,

Kaboli

Naserian

et al. 2020

Folia Histochem Cytobiol.

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Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one of the major genome editing systems and allows changing DNA levels of an organism. Among several CRISPR categories, the CRISPR-Cas9 system has shown a remarkable progression rate over its lifetime. Recently, other tools including CRISPR-Cas12 and CRISPR-Cas13 have been introduced. CRISPR-Cas9 system has played a key role in the industrial cell factory's production and improved our understanding of genome function. Additionally, this system has been used as one of the major genome editing systems for the diagnosis and treatment of several infectious and non-infectious diseases. In this review, we discuss CRISPR biology, its versatility, and its application in biomedical engineering.

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CRISPR technologies for stem cell engineering and regenerative medicine

Cited by 67 publications

References 215 publications

Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells

Repurposing type I–F CRISPR–Cas system as a transcriptional activation tool in human cells

Deciphering cell–cell interactions and communication from gene expression

An overview of applications of CRISPR-Cas technologies in biomedical engineering

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