Objectives: This study aims to (1) use the objective activPAL activity monitor to assess physical activity behaviors, including sitting/lying, standing, and both light (LIPA) and moderate-to-vigorous physical activity (MVPA); (2) to develop distinct activity profiles based on time spent in each behavior in a sample of adolescent females; and (3) examine whether levels of adiposity differ across these activity profiles. Methods: Female adolescents (n = 195; 14-18 y) had body mass index (median = 21.7 [IQR = 5.2] kg/m 2 ) and 4-site skinfold thickness (median 62.0 mm; IQR = 37.1) measured. Physical activity behaviors were measured using the activPAL. Hierarchical cluster analysis grouped participants into activity profiles based on similar physical activity characteristics. Linear mixed models explored differences in body composition across activity profiles. Results: Three activity profiles were identified, a low (n = 35), moderate (n = 110), and a high activity profile (n = 50). Significant differences across activity profiles were observed for skinfold thickness (p = .046), with higher values observed in the low activity profile compared with the high activity profile. Conclusions: Profiling free-living activity using behaviors from across the activity intensity continuum may account for more of the variability in energy expenditure then examining specific activity intensities, such as MVPA alone. The use of activity profiles may enable the identification of individuals with unhealthy activity behaviors, leading to the development and implementation of more targeted interventions.Keywords: sitting behavior, physical activity, activPAL, cluster analysis, activity profile, adolescents, obesity Page 2 of 9 Levels of childhood overweight and obesity are at epidemic proportions (43), with 43 million children estimated to be overweight or obese globally (5). Approximately 25% of European adolescents are overweight or obesity (22), while 16.9% of 2-19-yearolds in the United States are now considered obese (28). Overweight and obese children are at a significantly increased risk of becoming overweight and obese adults (34,37) and of developing a range of health consequences, including type 2 diabetes mellitus, cardiovascular disease in adulthood, and site-specific cancers (10).Population-based research has traditionally focused on examining the effects of moderate-to-vigorous physical activity (MVPA) on cardiovascular risk factors, including measures of adiposity (2,29). More recently, sedentary time (referred to as any waking behavior spent in a sitting/reclining position requiring an energy expenditure of 1.5 metabolic equivalents [38]) has also been identified as having an important role in the development of risk factors for cardiovascular disease in adults (29). The predominant method of examining associations between these physical activity behaviors, such as sedentary time or MVPA, and indices of health has been to focus on the specific behavior (i.e., sitting/lying time) while adjusting for some confounding v...