Critérios para o teste de tetrazólio na estimativa do potencial germinativo em macaúba

Abstract

Resumo -O objetivo deste trabalho foi estabelecer critérios para a aplicação do teste de tetrazólio em embriões de macaúba (Acrocomia aculeata). Para a elaboração do esquema de avaliação, foram realizadas avaliações anatômicas, identificação de padrões de coloração por solução de 2,3,5-trifenil cloreto de tetrazólio a 0,5% por quatro horas e cultivo in vitro de embriões. Em um experimento, foram avaliados três períodos de pré-condicionamento das sementes por imersão em água, por 12 e 24 horas, associados a duas temperaturas de coloração, 35 e 40ºC, em dois lotes de sementes além do controle sem pré-condicionamento. Em outro experimento, foram testadas em três lotes de sementes, três concentrações da solução de tetrazólio (0,5, 0,75 e 1%), associadas a dois tempos de coloração (duas e quatro horas). Utilizou-se o cultivo in vitro de embriões para comparação dos resultados. Um esquema de avaliação com dez padrões de coloração, associados a três classes de vigor, foi definido com base na anatomia dos embriões e no desenvolvimento de plântulas in vitro. O tratamento de pré-condicionamento em água não é necessário, e se deve aplicar o tempo de coloração de quatro horas, em solução de sal de tetrazólio, a 0,5% e 35ºC.Termos para indexação: Acrocomia aculeata, 2,3,5-trifenil cloreto de tetrazólio, cultivo in vitro de embriões. Criteria for tetrazolium tests in the estimationof the germination potential of macaw palmAbstract -The objective of this study was to establish criteria for the tetrazolium test in embryos of macaw palm (Acrocomia aculeata). To develop the evaluation scheme, anatomical analyses were performed, and staining patterns were identified using a 0.5% 2,3,5-triphenyl tetrazolium chloride solution for four hours, and embryo was cultured in vitro. In one experiment, three prior seed-soaking times in water (for 12 and 24 hours, besides a control without seed pre-conditioning), associated with two color temperatures (35 and 40°C) in two seed lots were evaluated. In another experiment, three concentrations of tetrazolium solution (0.5, 0.75 and 1%), associated with two staining times (two and four hours), were tested in three seed lots. In vitro embryo culture was used to compare the results. An evaluation scheme, with ten staining patterns associated with three classes of vigor, was defined, based on the anatomy of the embryo and on the in vitro seedling development. Pre-soaking is not necessary, and staining for four hours in a solution of tetrazolium salt, at 0.5% and 35ºC, should be used.

Seed quality evaluation by tetrazolium staining during a desiccation study of the recalcitrant seeds of Carapa guianensis Aubl. and Carapa surinamensis Miq. - Meliaceae

“…Mart. (Ribeiro et al, 2010) and 0.075% for Glycine max (L.) Merril (Zuffo et al, 2015). A concentration equal to this study (0.10% TZ) was used for seeds of Schizolobium parahyba Vell.…”

“…Mart. (Ribeiro et al, 2010) and Glycine max (L.) Merril (Zuffo et al, 2015). For Ricinus communis L. a staining period of 6 h is recommended (Oliveira et al, 2014a).…”

Effects of drying temperature on viability of macaw palm (Acrocomia aculeata) zygotic embryos

“…Embryos were soaked in deionised water (50 mL) for 24 h at 25°C for the measurement of electrical conductivity. The tetrazolium test was performed according to Ribeiro et al (2010).…”

“…The two factors are discussed here. The tetrazolium method proposed by Ribeiro et al (2010) was used to classify the embryos by colour, vigor, viable, unviable or dead.…”

“…The tetrazolium test applied according to Ribeiro et al (2010) resulted in staining of the embryos that was sufficient for the quantification of embryos' vigour. This test has helped researchers to know quickly and accurately the viability of embryos; thus, it was found that increasing the temperature result in decreased viability.…”