Critical assessment on structural analysis of scalp hair using scanning electron microscope (SEM) and compound microscope

Davis, Cavalera; Khofar, Putri Nabihah Abdul; Karim, Umi Kalsum Abdul; Rashid, Reena Abd; Mahat, Mohd Muzamir; Halim, Mohamed Izzharif Abdul

doi:10.1016/j.matpr.2020.05.538

Cited by 4 publications

(12 citation statements)

References 14 publications

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“…Hair characterization was performed with Hitachi SEM micrographs (type SU-3500) to generate microscopic images of hair 16 . A hair sample is prepared, washed, classified, and cut.…”

Section: Resultsmentioning

confidence: 99%

“…A hair sample is prepared, washed, classified, and cut. The hair section attached to the conductive tape is coated with gold film coater using a vacuum sputtering machine 23 at 10 kV 16 . Scanning was carried out on several sides to ensure that the changes in the surface of the hair sample looked uniform 16 .…”

Section: Resultsmentioning

confidence: 99%

“…The hair section attached to the conductive tape is coated with gold film coater using a vacuum sputtering machine 23 at 10 kV 16 . Scanning was carried out on several sides to ensure that the changes in the surface of the hair sample looked uniform 16 . Researchers used the degree of cuticle damage as a criterion for assessing hair damage.…”

Section: Resultsmentioning

confidence: 99%

“…Hair sample preparation: This process used the method by Cao et al 21 and Davis et al 16 . Hair samples were collected from a volunteer (ethical clearance No.…”

Section: Hair Morphology Analysismentioning

confidence: 99%

“…Clinically, microscopic analysis such as the scanning electron microscope (SEM) can assess hair damage by identifying the characteristic morphology 14, 15 . This method allows observations not only on the outer surface but also on the inside of the hair 16 . Hair damage methods can be done through exposure to UVB rays and chemical damage 17 .…”

Section: Introductionmentioning

confidence: 99%

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Morphology Analysis of Hair Photoinduced and Chemical Damaged After Treatment with Sappan Wood (Caesalpinia sappan L.) Hair Tonic using SEM

Sari,

Widyasari,

Astuti

2023

Borneo J Pharm

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Exposure to sunlight for a long time and repeatedly can also cause chemical and physical damage to human hair. Clinically, microscopic analysis using a scanning electron microscope (SEM) can assess hair damage by identifying the characteristic morphology of hair damage. This study aims to analyze the morphology of damaged hair chemically induced using 3% H2O2 and photoinduced UVB radiation before and after applying sappan wood (Caesalpinia sappan) hair tonic. The active ingredients used were ethanol extract, ethanol fraction, and chloroform-methanol fraction of C. sappan, which contains an antioxidant compound. Caesalpinia sappan simplicia was macerated using 96% ethanol and then partitioned using n-hexane. The ethanol fraction was then applied using vacuum column chromatography using chloroform : methanol (5 : 1) as eluent. The extracts and fractions were then formulated into hair tonic preparations. For SEM analysis, hair samples were coated with a sputter gold coater machine and divided into five treatments: undamaged hair, damaged hair with 3% H2O2 and UVB rays as a positive control, and treatment I, II, and III, in which the hair was damaged with 3% H2O2 and UVB rays respectively, then FI, FII, and FIII were applied, respectively (2000x magnification). Damaged hair with UVB induction shows moderate damage, and 3% H2O2 shows moderate to severe damage. The results in the treatment group show that the three hair tonics coated the hair cuticle, indicating an interaction with the hair fiber, and modified the cuticle by coating the cuticle.

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“…Hair characterization was performed with Hitachi SEM micrographs (type SU-3500) to generate microscopic images of hair 16 . A hair sample is prepared, washed, classified, and cut.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Hair sample preparation: This process used the method by Cao et al 21 and Davis et al 16 . Hair samples were collected from a volunteer (ethical clearance No.…”

Section: Hair Morphology Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Morphology Analysis of Hair Photoinduced and Chemical Damaged After Treatment with Sappan Wood (Caesalpinia sappan L.) Hair Tonic using SEM

Sari,

Widyasari,

Astuti

2023

Borneo J Pharm

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Ecofriendly hair dye extraction from wastewater pericarp processing by-products of water caltrop (Trapa quadrispinosa): Optimization and evaluation

Liu

Chen

Liu

et al. 2023

Sustainable Chemistry and Pharmacy

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Rice Derivatives in Hair Protecting

Centini,

Signori,

Francescon

et al. 2023

Cosmetics

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The research was carried out on a hair conditioner containing the following rice derivatives individually: rice germ oil, rice germ oil GX-N, and riceterol esters. To evaluate the protective efficacy of the three active ingredients chosen, the following techniques were used: FT-IR, SEM, stress–strain test, and polarized light microscopy analysis. The tests were carried out on natural Caucasian hair. The methodologies were found to be suitable for the evaluation and led to interesting results: the selected ingredients showed good properties in improving the hair. The conditioners containing the active ingredients restored the properties of the hair even when subjected to stress such as irradiation. In this case, the most effective was the rice germ oil GX-N.

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Critical assessment on structural analysis of scalp hair using scanning electron microscope (SEM) and compound microscope

Cited by 4 publications

References 14 publications

Morphology Analysis of Hair Photoinduced and Chemical Damaged After Treatment with Sappan Wood (Caesalpinia sappan L.) Hair Tonic using SEM

Morphology Analysis of Hair Photoinduced and Chemical Damaged After Treatment with Sappan Wood (Caesalpinia sappan L.) Hair Tonic using SEM

Ecofriendly hair dye extraction from wastewater pericarp processing by-products of water caltrop (Trapa quadrispinosa): Optimization and evaluation

Rice Derivatives in Hair Protecting

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