Critical comparison of aerogel TiO2 and P25 nanopowders: Cytotoxic properties, photocatalytic activity and photoinduced antimicrobial/antibiofilm performance

Thirunavukkarasu, Guru Karthikeyan; Báčová, Jana; Monfort, Olivier; Dworniczek, Ewa; Paluch, Emil; Hanif, Muhammad Bilal; Rauf, Sajid; Motlochová, Monika; Čapek, Jan; Hensel, Karol; Plesch, G.; Chodaczek, Grzegorz; Roušar, Tomáš; Motola, Martin

doi:10.1016/j.apsusc.2021.152145

“…The photocatalytic degradation of caffeine follows the first-order reaction. 36,37 Thus its kinetics can be expressed from the linear variation of ln C / C 0 ( C 0 and C is the initial and after the time concentration, respectively) as a function of time ( t ). The obtained photodegradation rates indicate that both materials prepared via anodization, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…The photocatalytic degradation of caffeine follows the first-order reaction. 36,37 Thus its kinetics can be expressed from the linear variation of ln C/C 0 (C 0 and C is the initial and after the time concentration, respectively) as a function of time (t). The obtained photodegradation rates indicate that both materials prepared via anodization, i.e., nanotubular TiO 2 (TNS-20, k = 0.0069 min −1 ) and porous TiO 2 (TNS-60, k = 0.0067 min −1 ), show an increased degradation rate of caffeine compared to TNS-40 (k = 0.0057 min −1 ) and TNS-80 (k = 0.0044 min −1 ).…”

Section: Catalysis Science and Technology Papermentioning

confidence: 99%

Anodization of large area Ti: a versatile material for caffeine photodegradation and hydrogen production

Šihor

¹

,

Hanif

²

,

Thirunavukkarasu

³

et al. 2022

Catal. Sci. Technol.

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“…A variety of TiO 2 NMs, eg nanoparticles, 21 nanofibers 17 and nanotubes, 22 has been developed. All these NMs have been evaluated for biological effects because TiO 2 has been used in medicine, material science and industry.…”

Section: Introductionmentioning

confidence: 99%

“…An essential topicality of present study can be also supported by frequent use of TiO 2 P25 as a comparative material for evaluation of biological effects in newly developed NMs. 21 , 37 , 41 , 42 …”

Section: Introductionmentioning

confidence: 99%

“…10 A549 cells have been widely used in reports estimating pulmonary toxicity of NMs, eg silica (SiO 2 ), iron oxides (Fe x O y ), zinc oxide (ZnO), titanium dioxide (TiO 2 ), [11][12][13] silver (Ag) 14 nanoparticles and other specifically functionalized nanoparticles, 15 nanofibers, 16,17 nanosheets 18 and carbon-based nanomaterials. 19,20 A variety of TiO 2 NMs, eg nanoparticles, 21 nanofibers 17 and nanotubes, 22 has been developed. All these NMs have been evaluated for biological effects because TiO 2 has been used in medicine, material science and industry.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Báčová¹,

Knotek²,

Kopecká³

et al. 2022

IJN

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Purpose Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO 2 P25), are among the most produced nanomaterials worldwide. The broad use of TiO 2 P25 in material science has implied a request to evaluate their biological effects, especially in the lungs. Hence, the pulmonary A549 cell line has been used to estimate the effects of TiO 2 P25. However, the reports have provided dissimilar results on caused toxicity. Surprisingly, the physicochemical factors influencing TiO 2 P25 action in biological models have not been evaluated in most reports. Thus, the objective of the present study is to characterize the preparation of TiO 2 P25 for biological testing in A549 cells and to evaluate their biological effects. Methods We determined the size and crystallinity of TiO 2 P25. We used four techniques for TiO 2 P25 dispersion. We estimated the colloid stability of TiO 2 P25 in distilled water, isotonic NaCl solution, and cell culture medium. We applied the optimal dispersion conditions for testing the biological effects of TiO 2 P25 (0–100 µg.mL −1 ) in A549 cells using biochemical assays (dehydrogenase activity, glutathione levels) and microscopy. Results We found that the use of fetal bovine serum in culture medium is essential to maintain sufficient colloid stability of dispersed TiO 2 P25. Under these conditions, TiO 2 P25 were unable to induce a significant impairment of A549 cells according to the results of biochemical and microscopy evaluations. When the defined parameters for the use of TiO 2 P25 in A549 cells were met, similar results on the biological effects of TiO 2 P25 were obtained in two independent cell laboratories. Conclusion We optimized the experimental conditions of TiO 2 P25 preparation for toxicity testing in A549 cells. The results presented here on TiO 2 P25-induced cellular effects are reproducible. Therefore, our results can be helpful for other researchers using TiO 2 P25 as a reference material.

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2D TiO₂ Nanosheets Decorated Via Sphere‐Like BiVO₄: A Promising Non‐Toxic Material for Liquid Phase Photocatalysis and Bacterial Eradication

Hanif,

Bacova,

Berezenko

et al. 2024

ChemSusChem

5

0

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An in‐depth investigation was conducted on a promising composite material (BiVO4/TiO2), focusing on its potential toxicity, photoinduced catalytic properties, as well as its antibiofilm and antimicrobial functionalities. The preparation process involved the synthesis of 2D‐TiO2 using the lyophilization method, which was subsequently functionalized with sphere‐like BiVO4. Finally, we developed BiVO4/TiO2 S‐scheme heterojunctions which can greatly promote the separation of electron‐hole pairs to achieve high photocatalytic performance. The evaluation of concentration‐ and time‐dependent viability inhibition was performed on human lung carcinoma epithelial A549 cells. This assessment included the estimation of glutathione levels and mitochondrial dehydrogenase activity. Significantly, the BiVO4/TiO2 composite demonstrated minimal toxicity towards A549 cells. Impressively, the BiVO4/TiO2 composite exhibited notable photocatalytic performance in the degradation of rhodamine B (k =0.135 min‐1) and phenol (k = 0.016 min‐1). In terms of photoinduced antimicrobial performance, the composite effectively inactivated both gram‐negative E. coli and gram‐positive E. faecalis bacteria upon 60‐min of UV‐A light exposure, resulting in a significant log6(log10CFU/mL) reduction in bacterial count. These promising results can be attributed to the unique 2D morphology of TiO2 modified by sphere‐like BiVO4, leading to an increased generation of (intracellular)hydroxyl radicals, which plays a crucial role in treatments of both organic pollutants and bacteria.

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Critical comparison of aerogel TiO2 and P25 nanopowders: Cytotoxic properties, photocatalytic activity and photoinduced antimicrobial/antibiofilm performance

Cited by 19 publications

References 76 publications

Anodization of large area Ti: a versatile material for caffeine photodegradation and hydrogen production

Anodization of large area Ti: a versatile material for caffeine photodegradation and hydrogen production

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

2D TiO₂ Nanosheets Decorated Via Sphere‐Like BiVO₄: A Promising Non‐Toxic Material for Liquid Phase Photocatalysis and Bacterial Eradication

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Critical comparison of aerogel TiO2 and P25 nanopowders: Cytotoxic properties, photocatalytic activity and photoinduced antimicrobial/antibiofilm performance

Cited by 19 publications

References 76 publications

Anodization of large area Ti: a versatile material for caffeine photodegradation and hydrogen production

Anodization of large area Ti: a versatile material for caffeine photodegradation and hydrogen production

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

2D TiO2 Nanosheets Decorated Via Sphere‐Like BiVO4: A Promising Non‐Toxic Material for Liquid Phase Photocatalysis and Bacterial Eradication

Contact Info

Product

Resources

About

2D TiO₂ Nanosheets Decorated Via Sphere‐Like BiVO₄: A Promising Non‐Toxic Material for Liquid Phase Photocatalysis and Bacterial Eradication