Critical comparison of direct analysis in real time orbitrap mass spectrometry (DART-Orbitrap MS) towards liquid chromatography mass spectrometry (LC-MS) for mycotoxin detection in cereal matrices

Tsagkaris, Aristeidis S.; Hrbek, Vojtěch; Džuman, Zbyněk; Hajšlová, Jana

doi:10.1016/j.foodcont.2021.108548

Cited by 20 publications

(6 citation statements)

References 30 publications

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“…It is also noticeable that the spectrum for the temperature of 400 °C showed ion suppression effects of compounds with low intensity, as it favored the ionization of other compounds while making it impossible to assess NDEA. This ion suppression effect occurs with analytes in DART-MS analyses due to competition for ionization among analytes thermally desorbed by the DART source since matrix analytes are thermally desorbed all at once. , Thus, low temperatures minimize this effect as fewer amount of analytes are desorbed from the matrix.…”

Section: Resultsmentioning

confidence: 61%

An Agile and Accurate Approach for N-Nitrosamines Detection and Quantification in Medicines by DART-MS

Batista Junior,

Bernardo,

Rocha

et al. 2024

J. Am. Soc. Mass Spectrom.

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N-nitrosamines (NAs) are prevalent mutagenic impurities in various consumer products. Their discovery in valsartan-containing medicines in 2018 prompted global regulatory agencies to set guidelines on their presence and permissible levels in pharmaceuticals. In order to determine the NAs content in medicines, efficient and sensitive analytical methods have been developed based on mass spectrometry techniques. Direct analysis in real time-mass spectrometry (DART-MS) has emerged as a prominent ambient ionization technique for pharmaceutical analysis due to its high-throughput capability, simplicity, and minimal sample preparation requirements. Thus, in this study DART-MS was evaluated for the screening and quantification of NAs in medicines. DART-MS analyses were conducted in positive ion mode, for both direct tablet analysis and solution analysis. The analytical performance was evaluated regarding linearity, precision, accuracy, limits of detection, and quantification. The DART-MS proved to be suitable for the determination of NAs in medicines, whether through direct tablet analysis or solution analysis. The analytical performance demonstrated linearity in the range from 1.00 to 200.00 ng mL −1 , limits of quantification about 1.00 ng mL −1 , precision and accuracy lower than 15%, and no significant matrix effect for six drug-related NAs. In conclusion, the DART-MS technique demonstrated to be an alternative method to determine NAs in medicines, aligning with the principles of green chemistry.

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Section: Resultsmentioning

confidence: 61%

An Agile and Accurate Approach for N-Nitrosamines Detection and Quantification in Medicines by DART-MS

Batista Junior,

Bernardo,

Rocha

et al. 2024

J. Am. Soc. Mass Spectrom.

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“…The analyte list contained 19 mycotoxins (Figure 1), namely, 3 regulated mycotoxins (ochratoxin A, deoxynivalenol and zearalenone) and 16 non-regulated mycotoxins, including 11 ergot alkaloids (EAs). In contrast to our recent study that focused on mycotoxin determination using ambient MS [12], in which the EA concentration was reported as a sum, in this case the EA epitopes can be effectively identified and quantified. In addition, all the detected mycotoxins are considered compounds with significant toxicity, resulting in potential health effects upon certain dietary exposure.…”

Section: Introductionmentioning

confidence: 71%

Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method

Tsagkaris

Prusova

Džuman

et al. 2021

Toxins

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Cereals represent a widely consumed food commodity that might be contaminated by mycotoxins, resulting not only in potential consumer health risks upon dietary exposure but also significant financial losses due to contaminated batch disposal. Thus, continuous improvement of the performance characteristics of methods to enable an effective monitoring of such contaminants in food supply is highly needed. In this study, an ultra-high-performance liquid chromatography coupled to a hybrid quadrupole orbitrap mass analyzer (UHPLC-q-Orbitrap MS) method was optimized and validated in wheat, maize and rye flour matrices. Nineteen analytes were monitored, including both regulated mycotoxins, e.g., ochratoxin A (OTA) or deoxynivalenol (DON), and non-regulated mycotoxins, such as ergot alkaloids (EAs), which are analytes that are expected to be regulated soon in the EU. Low limits of quantification (LOQ) at the part per trillion level were achieved as well as wide linear ranges (four orders of magnitude) and recovery rates within the 68–104% range. Overall, the developed method attained fit-for-purpose results and it highlights the applicability of high-resolution mass spectrometry (HRMS) detection in mycotoxin food analysis.

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“…To extract the pesticide residues from the cereal matrices, a Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) approach previously used in mycotoxin analysis by our group, was applied. 15 In the next step, 10 mL of acetonitrile were added and the tube was vigorously shaken by hand for 2 min. This was followed by addition of 4 g of anhydrous magnesium sulphate (MgSO 4 , Fluka, Buchs, Germany) and 1 g of sodium chloride (NaCl, Penta, Chrudim, Czech Republic) and the tube was vigorously shaken by hand for 1 min.…”

Section: Methodsmentioning

confidence: 99%

A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices

et al. 2022

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Critical comparison of direct analysis in real time orbitrap mass spectrometry (DART-Orbitrap MS) towards liquid chromatography mass spectrometry (LC-MS) for mycotoxin detection in cereal matrices

Cited by 20 publications

References 30 publications

An Agile and Accurate Approach for N-Nitrosamines Detection and Quantification in Medicines by DART-MS

An Agile and Accurate Approach for N-Nitrosamines Detection and Quantification in Medicines by DART-MS

Regulated and Non-Regulated Mycotoxin Detection in Cereal Matrices Using an Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) Method

A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices

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