Influence of gamma irradiation to the magneto-impedance ratio of the electrodeposited [NiFe/Cu]4 multilayer on meander-shape PCB substrate has been studied. The magneto-impedance ratios were measured for both un-irradiated and irradiated by gamma radiation of Co-60 with a total dose of 40 Gy. The morphological structure were done by using scanning electron microscopy (SEM). The decrease in grain size of the samples after Gamma irradiated is observed. The magnetic property modifies as consequence of the change in the microstructure samples. Within result, the magneto-impedance ratio decreases around 34.9% for irradiated sample.