Critical evaluation of the applicability of neutral carrier-based calcium selective microelectrodes

Lanter, F.; Steiner, R. A.; Ammann, D.; Simon, W.

doi:10.1016/s0003-2670(01)85264-8

Cited by 77 publications

(25 citation statements)

References 45 publications

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“…PVC tubing . The detection limit of such electrodes is^-1 nM (Lanter et al, 1982) . The macro PVC electrodes were used to check the final concentrations of free Ca in the calibrating solutions .…”

Section: Area Of Response To Test Flashesmentioning

confidence: 99%

Relationship between light sensitivity and intracellular free Ca concentration in Limulus ventral photoreceptors. A quantitative study using Ca-selective microelectrodes.

Levy¹,

Fein²

1985

The Journal of General Physiology

101

106

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The possible role of Ca ions in mediating the drop insensitivity associated with light adaptation in Limulus ventral photoreceptors was assessed by simultaneously measuring the sensitivity to light and the intracellular free Ca concentration (Ca i) ; the latter was measured by using Ca-selective microelectrodes . In dark-adapted photoreceptors, the mean resting Cai was 3 .5 ± 2 .5,M SD (n = 31) . No correlation was found between resting Cai and absolute sensitivity from cell to cell . Typically, photoreceptors are not uniformly sensitive to light ; the Cai rise evoked by uniform illumination was 20-40 times larger and faster in the most sensitive region of the cell (the rhabdomeral lobe) than it was away from it . In response to a brief flash, the Cai rise was barely detectable when 10 2 photons were absorbed, and it was saturated when^-10 5 photons were absorbed . During maintained illumination, starting near the threshold of light adaptation, steady Cai increases were associated with steady desensitizations over several log units of light intensity : a 100-fold desensitization was associated with a 2 .5-fold increase in Ca ; . After a bright flash, sensitivity and Cai recovered with different time courses : the cell was still desensitized by -0 .5 log units when Ca i had already recovered to the prestimulus level, which suggests that under those conditions Cai is not the rate-limiting step of dark adaptation . Ionophoretic injection of EGTA markedly decreased the light-induced Cai rise and increased the time to peak of the light response, but did not alter the resting Ca;, which suggests that the time to peak is affected by a change in the capacity to bind Ca2' and not by resting Ca i . Lowering the extracellular Ca 21 concentration (Ca .) first decreased Cai and increased sensitivity. Longer exposure to low Ca. resulted in a further decrease of Ca ; but decreased rather than increased Address reprint requests to Dr.

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Section: Area Of Response To Test Flashesmentioning

confidence: 99%

Relationship between light sensitivity and intracellular free Ca concentration in Limulus ventral photoreceptors. A quantitative study using Ca-selective microelectrodes.

Levy¹,

Fein²

1985

The Journal of General Physiology

101

106

View full text Add to dashboard Cite

show abstract

“…Unlike most fluorescent or luminescent indicators, CaSMs were originally developed for measuring both intracellular and extracellular [Ca 2+ ] (listed in Lanter et al, 1982). Measurement of minute Ca 2+ gradients on the outside of cells was limited by electrical drift in the system.…”

Section: +mentioning

confidence: 99%

“…A primary motivation for early development of Ca 2+ -selective microelectrodes was to monitor intracellular [Ca 2+ ] (Tsien and Rink, 1981;Lanter et al, 1982). This required a liquid membrane with high selectivity for monitoring the low resting [Ca 2+ ] i (~100 nM) in the presence of higher concentrations of potentially interfering ions including K + (~120 mM), Na + (~10 mM) and Mg 2+ (~1 mM).…”

Section: B Selectivitymentioning

confidence: 99%

“…This required a liquid membrane with high selectivity for monitoring the low resting [Ca 2+ ] i (~100 nM) in the presence of higher concentrations of potentially interfering ions including K + (~120 mM), Na + (~10 mM) and Mg 2+ (~1 mM). Accordingly, 2 different Ca 2+ ionophores with very high selectivity were reported (Ammann et al, 1975;Lanter et al, 1982;Ammann et al, 1987). Some of their selectivity coefficients for Ca 2+ over other common cations are listed in Table 1.…”

Section: B Selectivitymentioning

confidence: 99%

“…(Breitwieser, 2008). Ca 2+ selective microelectrodes have been instrumental in providing the sensitivity for defining the complex transport of the Na + /Ca 2+ exchanger (Kang and Hilgemann, 2004) and the P-type plasma membrane Ca 2+ pump (PMCA) in neurons (Thomas, 2009 (Lanter et al, 1982) 2 (Ammann et al, 1975) 3 (Ammann et al, 1987) Response Times (t 95% ms)…”

Section: Ca 2+ Flux Measurementsmentioning

confidence: 99%

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Smith

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Errachid¹,

Zine

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Miniaturized solid-state ion sensors based on field-effect transistors on silicon can take advantage of the capabilities of microelectronics and microsystems technology for the integration of combined functionalities. Optimized solidstate chemical sensors usually require specific materials and fabrication processes. However, if standard fabrication processes can be used, integrated chemical sensor systems can be developed in a shorter time and in a cost effective way. We show that, for applications in which a long operating time is not required, such as in disposable biomedical sensors, good integrated sensor systems can be fabricated with standard materials and processes. Specific examples are presented, such as multisensor systems, sensors with integrated signal-processing circuits and sensors with particle manipulation electrodes.

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Critical evaluation of the applicability of neutral carrier-based calcium selective microelectrodes

Cited by 77 publications

References 45 publications

Relationship between light sensitivity and intracellular free Ca concentration in Limulus ventral photoreceptors. A quantitative study using Ca-selective microelectrodes.

Relationship between light sensitivity and intracellular free Ca concentration in Limulus ventral photoreceptors. A quantitative study using Ca-selective microelectrodes.

Construction, Theory, and Practical Considerations for using Self-referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients

FET‐Based Chemical Sensor Systems Fabricated with Standard Technologies

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