Critical evaluation of the TDx Turbo Nephelometry System.

Abstract

We critically evaluated the Nephelometry System (TDx Turbo) for the TDx Analyzer (Turbo), as currently used for immunoglobulins (IgA, IgG, and IgM) and transferrin assays. We describe hardware, software, reagent, and procedural changes made since the original publication (Clin Chem 1985;31:1474) and the mathematical treatment of data. The unit-dose format of the reagents allows for batch or panel testing of the analytes, with a throughput of 40 tests per hour. Measurements are made at pseudo-equilibrium, with sample-blank correction. The formulation of reagents and the assay scheme are such as to eliminate the need to check these assays for antigen excess. Assay sensitivities are 3% of the standard curve ranges. CVs are 8% for all assays. The system performance, when compared with rate nephelometry and radial immunodiffusion, yielded correlation coefficients of 0.97 or better for the analytes.

Factors Affecting High-Pressure Solvent Extraction (Accelerated Solvent Extraction) of Additives from Polymers

“…There are several new techniques of extraction that have been widely applied to environmental samples which reduce both extraction time and solvent usage. These are supercritical fluid extraction, microwave-assisted extraction (MAE), and accelerated solvent extraction (ASE), which have been described in recent articles. − (ASE is a trademark of the Dionex Corp. but will be used here, by permission, to refer to high-pressure solvent extraction, using any experimental system.) These techniques can all be used above atmospheric pressure and could therefore be called high-pressure extraction methods.…”