Critical factors affecting an efficient micropropagation protocol for  Pyrus spinosa  Forskk.

Tsoulpha, Parthena; Alexandri, S.; Τσακτσίρα, Μαρία

doi:10.37855/jah.2018.v20i03.33

Cited by 5 publications

(9 citation statements)

References 22 publications

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“…From an ecological point of view, the almond-leaved pear is a xerophyle species, with a high level of adaptability to different climatic and soil conditions, including extreme conditions like dry, warm, and degraded habitats [19,24,27,28]. It grows on sandy, clay, stony, and calcareous soils [7,29], sometimes even with a high salt level [30].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Diversity of Almond-Leaved Pear (Pyrus spinosa Forssk.) along Eastern Adriatic Coast

Vidaković

Liber

Šatović

et al. 2021

Forests

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Almond-leaved pear (Pyrus spinosa Forssk., Rosaceae) is a scientifically poorly researched and often overlooked Mediterranean species. It is an insect-pollinated and animal-dispersed spiny, deciduous shrub or a small tree, with high-quality wood and edible fruits. The aim of the study was to assess the phenotypic diversity of almond-leaved pear in the eastern Adriatic region. The examination of phenotypic diversity was based on a morphometric analysis of 17 populations using ten phenotypic traits of leaves. Varieties of multivariate statistical analyses were conducted to evaluate the within- and among-population diversity. In addition, the Mantel tests were used to test the correlations between geographic, environmental, and phenotypic differences among populations. High phenotypic variability was determined both among and within the studied populations. Leaf-size-related traits proved to be the most variable ones, in contrast to more uniform leaf shape traits. Furthermore, three groups of populations were detected using multivariate statistical analyses. The first group included trees from northern- and southernmost populations characterized by high annual precipitation. However, the trees from the second and third group were highly overlapped without a clear geographical pattern. In addition, we revealed that both environmental and geographical interactions proved to be responsible for the patterns of phenotypic variation between almond-leaved pear populations, indicating significant isolation by environment (IBE) and isolation by distance (IBD) patterns. Overall, our results provide useful information about phenotypic diversity of almond-leaved pear populations for further conservation, breeding, and afforestation programs.

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Section: Introductionmentioning

confidence: 99%

Phenotypic Diversity of Almond-Leaved Pear (Pyrus spinosa Forssk.) along Eastern Adriatic Coast

Vidaković

Liber

Šatović

et al. 2021

Forests

View full text Add to dashboard Cite

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“…Winter shoots of the following year (February) were collected and kept in vessels with tap water for 20 days under controlled environmental conditions in a plant growth chamber (16 h photoperiod, temperature 24 ± 1 • C and light irradiance of 35 µmol•m −2 •s −1 ). The shoot tips of the newly emerged shoots (1.5-2.0 cm long) from the lateral buds were isolated and used for in vitro culture initiation after disinfection by following the procedure of Tsoulpha et al [2].…”

Section: Establishment Of In Vitro Cultures and Shoot Proliferationmentioning

confidence: 99%

“…Pyrus spinosa Forssk. is a small, deciduous wild-growing tree belonging to the Rosaceae, which is widely spread in the Mediterranean basin and the Greek countryside [1,2]. Previously, the species was known as P. amygdaliformis Vill for the characteristic almond-shaped form of its leaves.…”

Section: Introductionmentioning

confidence: 99%

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Selection for Sustainable Preservation through In Vitro Propagation of Mature Pyrus spinosa Genotypes Rich in Total Phenolics and Antioxidants

et al. 2023

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Pyrus spinosa Forssk. (almond-leaved pear) is a wild-growing native tree of the hilly countryside of Greece, which has recently gained interest for its valuable endogenous substances. In the present work, the determination of the total phenol content (TPC) and antioxidant capacity (AC) was carried out during 2021 and 2022 in leaves from 32 genotypes of mature wild-growing trees at four different locations of Central Macedonia District, Greece: Agia Anastasia, Chalkidiki, Lagadas and Pieria. The measurements taken from the leaf samples of all genotypes revealed a strong positive linear correlation between TPC and AC (R2 = 0.772). Two genotypes from Agia Anastasia, coded as AA2 and AA3, were comparatively the richest in TPC (44.86 for AA2 and 46.32 mg GAE/g fresh weight for AA3) and AC (70.31 and 71.21 μg AAE/g fresh weight for the same genotypes). For these two genotypes of high TPC and AC, an efficient micropropagation protocol was developed to preserve and multiply this valuable germplasm. Newly emerged shoot tips were excised from winter shoots and, after disinfestation, they were established on a modified Murashige and Skoog (MS) nutrient medium with 5 μΜ of 6-Benzyloaminopurine (ΒΑP). For shoot multiplication, explants from clean cultures were transferred to Pear Medium 1 with 5 μΜ of BAP. The effect of the nutrient medium was tested by using five different nutrient media (modified MS, Pear Medium 1, Pear Medium 2, DKW and WPM) supplemented with 5 μΜ of BAP. Pear Medium 1 was the most effective in shoot formation. Among the four different BAP concentrations (0, 5, 10 and 20 μΜ) used in Pear Medium 1, 5 μΜ of BAP resulted in the production of the significantly highest number of shoots (22.7 shoots per explant, 2.4 cm long). The exposure of cultures to 10 μmol·m−2·s−1 for one week under light irradiance followed by 35 μmol·m−2·s−1 for four weeks increased both the number and length of the shoots produced. A 20-sec dip of the shoot bases into 49.0 μΜ of Indole-3-butyric acid (IBA) in EtOH equally affected rooting in a modified rooting MS (79%) and Pear Medium 1 (80%). The root system developed better in the modified rooting MS medium (mean number of roots of 2.7 with a length of 3.9 cm). The acclimatization of the plantlets was successfully carried out (high survival rates five weeks after their establishment) on a perlite substrate under controlled environmental conditions.

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“…In the present work, the problem of explant browning and necrosis noticed at this stage was eliminated by immersing plant material in an antioxidant solution (ascorbic acid and citric acid). The same practice was successfully followed for another member of the Lamiaceae family, Rosmarinus officinalis [11] and one of the Rosaceae, Pyrus spinosa [45]. It is known that ascorbic acid prevents the browning and hyperhydricity of explants and improves in vitro rooting and ex vitro survival of the plants [17,46,47].…”

Section: In Vitro Propagation Of S Officinalis Effect Of Disinfection...mentioning

confidence: 99%

Asexual Propagation of Greek Salvia officinalis L. Populations Selected for Ornamental Use

et al. 2023

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Salvia officinalis, commonly known as sage, is highly valued for its medicinal and ornamental properties. In the present work, 12 native sage populations of north-west Greece were evaluated for eight ornamental traits. Populations from the locations of Aristi, Kefalovryso and Igoumenitsa were selected as the best performing and for their preservation and availability in the market, their asexual propagation was investigated by (a) shoot cutting and (b) in vitro techniques. Propagation by cuttings was investigated during the four seasons. Aristi exhibited the highest rooting (65%) in spring with a well-developed root system (4.7 root number and 5.0 cm length) by applying 0.5 g L−1 Indole-3-butyric acid, potassium salt (K-IBA), established on perlite under a fog system. However, the rooting performance of Aristi spring cuttings was not affected by different substrates of peat:perlite (0:1, 1:1, 1:2 v/v) or rooting systems (mist, fog) tested. Furthermore, the in vitro propagation of the selected sage populations was investigated using shoot tips as explants. After successful disinfection, the effect of Murashige and Skoog (MS) medium in ten different combinations of Indole-3-acetic acid (IAA), 6-Benzylaminopurine (BAP) and Thidiazuron (TDZ) were tested on shoot multiplication. Aristi presented the highest number of newly formed shoots on MS9 (0.1 mg L−1 IAA and 0.8 mg L−1 TDZ) and MS5 (0.1 mg L−1 IAA and 0.8 mg L−1 BAP) (3.35 and 3.21 new shoots/explant, respectively) with the highest shoot length (2.23 cm and 3.2 cm) and unexpected spontaneous root formation (64%) at MS5. The rooting ability of Aristi microshoots was further investigated in order to enhance their response. Of the three rooting variants tested, optimal rooting formation (100%) was observed on 0.9 mg L−1 IAA (R3) combined with successful acclimatization (100%). Aristi exceeded the other populations in both the tested propagation systems, thus holding a strong potential for its introduction in the market as a competitive ornamental variety.

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Critical factors affecting an efficient micropropagation protocol for Pyrus spinosa Forskk.

Cited by 5 publications

References 22 publications

Phenotypic Diversity of Almond-Leaved Pear (Pyrus spinosa Forssk.) along Eastern Adriatic Coast

Phenotypic Diversity of Almond-Leaved Pear (Pyrus spinosa Forssk.) along Eastern Adriatic Coast

Selection for Sustainable Preservation through In Vitro Propagation of Mature Pyrus spinosa Genotypes Rich in Total Phenolics and Antioxidants

Asexual Propagation of Greek Salvia officinalis L. Populations Selected for Ornamental Use

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