We report the effect of La-substitution on the magnetic and magnetotransport properties of Brownmillerite-like bilayered compounds Ca 2.5-x La x Sr 0.5 GaMn 2 O 8 (x = 0, 0.05, 0.075, and 0.1) by using dc-magnetization, resistivity and magnetoresistance techniques. The Rietveld analysis of the room temperature x-ray diffraction patterns confirms no observable change of average crystal structure with the La-substitution. Both magnetic and magnetotransport properties are found to be very sensitive to the La-substitution. Interestingly, the La-substituted compounds show ferromagnetic-like behavior (due to the occurrence of a double exchange mechanism) whereas, the parent compound is an antiferromagnet (T N~1 50 K). All compounds show an insulating behavior, in the measured temperature range of 100 -300 K, with an overall decrease in the resistivity with the substitution. A higher value of magnetoresistance has been successfully achieved by the La-substitution. We have proposed an electronic phase separation model, considering the formation of ferromagnetic clusters in the antiferromagnetic matrix, to interpret the observed magnetization and magnetotransport results for the La-substituted samples. The present study demonstrates an approach to achieve new functional materials, based on naturally occurring layered system like Ca 2.5-x La x Sr 0.5 GaMn 2 O 8 , for possible spintronics applications.