Field studies were conducted in the northeastern part of the Republic of Croatia to determine the influence of the critical period of weed interference on sunflower (Helianthus annuus) yield, yield components, and oil content. For this purpose, different durations of competition were established, allowing weeds to infest the crop for increasing periods of time after planting or maintaining plots weed-free for increasing periods of time after planting. The beginning and the end of the critical period of weed control (CPWC), based on a 5% and 10% loss of sunflower yield, were determined by fitting the four-parameter log-logistic equations to the relative seed yield. The total weed biomass increased progressively in relation to the increase in the competition. The beginning of the CPWC period, based on a 5% acceptable yield loss, ranged from 141 to 234 growing degree days (GDD), which corresponded to the two-to-four true leaf development stage (the V2–V4 growth stages) across both sites and years. The crop had to be kept weed-free until a period when sunflower inflorescence began to open and flower (the R4–R5 growth stage) or from 1365 to 1932 GDD. The sunflower yield and yield components varied between the years and among locations. An increasing duration of weed interference negatively affected crop height, head diameter, and 1000-kernel weight, but not seed oil content.