Critical points for optimizing long-term culture and neural differentiation capacity of rodent and human neural stem cells to facilitate translation into clinical settings

Radoszkiewicz, Klaudia; Hribljan, Valentina; Isakovic, Jasmina; Mitrečić, Dinko; Sarnowska, Anna

doi:10.1016/j.expneurol.2023.114353

Cited by 7 publications

(3 citation statements)

References 207 publications

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“…However, it is known that cell culture protocols are not strictly standardized, which may lead to many still poorly investigated differences. These have been recently reported by our group [ 1 ] and others [ 16 ].…”

Section: Discussionsupporting

confidence: 83%

“…The growth of various types of cells is an obligatory part of the in vitro modeling of any neurological disease. Cell culture techniques frequently incorporate various supplements, as is known, and it has been demonstrated by our group that they can drastically alter cells’ phenotype [ 1 ]. Moreover, for some supplements, it has been shown that they can have protective effects.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment

Petrović,

Jagečić,

Krasić

et al. 2023

IJMS

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In vitro cell culture is a routinely used method which is also applied for in vitro modeling of various neurological diseases. On the other hand, media used for cell culture are often not strictly standardized between laboratories, which hinders the comparison of the obtained results. Here, we compared the effects of homocysteine (Hcy), a molecule involved in neurodegeneration, on immature cells of the nervous system cultivated in basal medium or media supplemented by either fetal bovine serum or basic fibroblast growth factor. The number of cells in basal media supplemented with basic fibroblast growth factor (bFGF) was 2.5 times higher in comparison to the number of cells in basal media supplemented with fetal bovine serum (FBS). We also found that the neuron-specific β-3-tubulin protein expression dose dependently decreased with increasing Hcy exposure. Interestingly, bFGF exerts a protective effect on β-3-tubulin protein expression at a concentration of 1000 µM Hcy compared to FBS-treated neural stem cells on Day 7. Supplementation with bFGF increased SOX2 protein expression two-fold compared to FBS supplementation. GFAP protein expression increased five-fold on Day 3 in FBS-treated neural stem cells, whereas on Day 7, bFGF increased GFAP expression two-fold compared to FBS-treated neural stem cells. Here, we have clearly shown that the selection of culturing media significantly influences various cellular parameters, which, in turn, can lead to different conclusions in experiments based on in vitro models of pathological conditions.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment

Petrović,

Jagečić,

Krasić

et al. 2023

IJMS

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“…The application of ESCs or autologous stem cells for cell therapy is compromised for both ethical and practical reasons. However, the discovery of a method to obtain, from adult somatic cells, patient-specific iPSCs from which NSC can then be obtained and maintained [ 9 ] opens the possibility for personalized NSC or trophic-factor therapies [ 10 ]. The use of differentiated/somatic cells is also possible, as reported by Ma and colleagues in their clinical trial [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Designing Electrical Stimulation Platforms for Neural Cell Cultivation Using Poly(aniline): Camphorsulfonic Acid

et al. 2023

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Electrical stimulation is a powerful strategy to improve the differentiation of neural stem cells into neurons. Such an approach can be implemented, in association with biomaterials and nanotechnology, for the development of new therapies for neurological diseases, including direct cell transplantation and the development of platforms for drug screening and disease progression evaluation. Poly(aniline):camphorsulfonic acid (PANI:CSA) is one of the most well-studied electroconductive polymers, capable of directing an externally applied electrical field to neural cells in culture. There are several examples in the literature on the development of PANI:CSA-based scaffolds and platforms for electrical stimulation, but no review has examined the fundamentals and physico-chemical determinants of PANI:CSA for the design of platforms for electrical stimulation. This review evaluates the current literature regarding the application of electrical stimulation to neural cells, specifically reviewing: (1) the fundamentals of bioelectricity and electrical stimulation; (2) the use of PANI:CSA-based systems for electrical stimulation of cell cultures; and (3) the development of scaffolds and setups to support the electrical stimulation of cells. Throughout this work, we critically evaluate the revised literature and provide a steppingstone for the clinical application of the electrical stimulation of cells using electroconductive PANI:CSA platforms/scaffolds.

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Phase I clinical trial of intracerebroventricular transplantation of allogeneic neural stem cells in people with progressive multiple sclerosis

Leone,

Gelati,

Profico

et al. 2023

Cell Stem Cell

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Critical points for optimizing long-term culture and neural differentiation capacity of rodent and human neural stem cells to facilitate translation into clinical settings

Cited by 7 publications

References 207 publications

Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment

Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment

Designing Electrical Stimulation Platforms for Neural Cell Cultivation Using Poly(aniline): Camphorsulfonic Acid

Phase I clinical trial of intracerebroventricular transplantation of allogeneic neural stem cells in people with progressive multiple sclerosis

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