Critical review of antibiotic resistance genes in the atmosphere

Kormos, David; Lin, Kaisen; Pruden, Amy; Marr, Linsey C.

doi:10.1039/d2em00091a

Cited by 25 publications

(7 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistance to quinolones is most often reported, mainly based on point mutations, while tetracycline resistance is the second most commonly reported, based on acquired genes located on plasmids or in the nucleoid [ 20 ]. In addition to a possible increased need for tetracycline as a treatment option, environmental screenings confirm that tetracycline and its metabolites are widespread, as well as tetracycline-resistant bacteria [ 21 ]. As one of the first resistance mechanisms studied in detail, tetracycline resistance genes now offer the opportunity to study evolutionary aspects in bacteria based on sequence data from the entire genome [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Low-Level Tetracycline Resistance Gene tet(O)_3 in Campylobacter jejuni

2023

View full text Add to dashboard Cite

Campylobacter (C.) spp. are the most important foodborne, bacterial, and zoonotic pathogens worldwide. Resistance monitoring of foodborne bacterial pathogens is an important tool to control antimicrobial resistance as a part of the “One Health” approach. The detection and functionality of new resistance genes are of paramount importance in applying more effective screening methods based on whole genome sequencing (WGS). Most tetracycline-resistant C. spp. isolates harbor tet(O), a gene that encodes a ribosomal protection protein. Here we describe tet(O)_3, which has been identified in two food isolates of C. jejuni and is very similar to the tet(O) gene in Streptococcus pneumoniae, having a truncated promoter sequence. This gene confers resistance to tetracycline below 1 mg/L, which is the epidemiological cut-off value. We have analyzed the entire genome of these two isolates, together with a C. jejuni isolate found to have high-level resistance to tetracycline. In contrast to the highly resistant isolate, the promoter of tet(O)_3 is highly responsive to tetracycline, as observed by reverse transcription polymerase chain reaction (RT-PCR). In addition, the two isolates possess a CRISPR repeat, fluoroquinolone resistance due to the gyrA point mutation C257T, a β-lactamase resistance gene blaOXA-184, a multidrug efflux pump CmeABC and its repressor CmeR, but no plasmid. Low-level antibiotic resistant C. jejuni might therefore have an advantage for surviving in non-host environments.

show abstract

Section: Discussionmentioning

confidence: 99%

Low-Level Tetracycline Resistance Gene tet(O)_3 in Campylobacter jejuni

2023

View full text Add to dashboard Cite

show abstract

“…But even habitats with no direct anthropogenic impact are regularly exposed to lower frequencies of such invasion events, for example, through wildlife and aerial depositions. These natural diffuse sources of AMR-pollution promote the widespread dispersal of AMR over time at low levels [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Microbiome diversity: A barrier to the environmental spread of antimicrobial resistance?

Klümper

Gionchetta

Catão

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: In the environment, microbial communities are constantly exposed to invasion by antimicrobial resistant bacteria (ARB) and their associated antimicrobial resistance genes (ARGs) that were enriched in the anthroposphere. A successful invader has to overcome the biotic resilience of the habitat, which is more difficult with increasing biodiversity. The capacity to exploit resources in a given habitat is enhanced when communities exhibit greater diversity, reducing opportunities for invaders, leading to a lower persistence. In the context of antimicrobial resistance (AMR) dissemination, exogenous ARB reaching a natural community may persist longer if the biodiversity of the autochthonous community is low, increasing the chance of ARGs to transfer to community members. Reciprocally, high microbial diversity could serve as a natural long-term barrier towards invasion by ARB and ARGs. Results: To test this hypothesis, a sampling campaign across seven European countries was carried out to obtain 172 environmental samples from sites with low anthropogenic impact. Samples were collected from contrasting environments: stationary structured forest soils, or dynamic river biofilms and sediments. Microbial diversity and relative abundance of 27 ARGs and 5 mobile genetic element marker genes were determined. In soils, higher diversity, evenness and richness were all significantly negatively correlated with the relative abundance of the majority (>85%) of ARGs. Furthermore, the number of detected ARGs per sample was inversely correlated with diversity. However, no such effects were found for the more dynamic, regularly mixed rivers. Conclusions: In conclusion, we demonstrate that diversity can serve as barrier towards AMR dissemination in the environment. This effect is mainly observed in stationary, structured environments, where long-term, diversity-based resilience against invasion can evolve. Such barrier effects can in the future be exploited to limit the environmental proliferation of AMR.

show abstract

“…ARGs belonging to glycopeptides and β-lactam families were the most common in hospital infection, while ARGs belonging to the sulfonamides and tetracyclines families were common in reports from farms, wastewater treatment plants (WWTPs), water and soil [7]. The ARGs adsorbed on PM2.5 might spread for a long distance in the atmosphere [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Can PM2.5 Exposure Change the Expression of ARGs in Intestinal Flora of Wistar Rats?

Liu¹,

Cheng²,

Zhang³

et al. 2023

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

The mechanism of PM2.5 in the air on health is complicated, in which the gut flora may play a role. The work aimed to study the changes of intestinal microflora in PM2.5 inhaled rats, further, to analyze the expression of antibiotic resistance genes (ARGs) in intestinal flora of the rats. The PM2.5 in the air of Fujian Province of China was collected to establish the rat model of PM2.5 inhaled. The fecal samples were sequenced by metagenomic method. Intestinal flora species annotation, common function database annotation and ARGs were analyzed between PM2.5 inhaled rats and normal control rats. The results showed that the number of Firmicutes was significantly decreased while that of Bacteroides was increased in the PM2.5 inhaled rats compared with the control rats at phylum level. In addition, from genus level to species level, the composition of intestinal flora in PM2.5 inhaled group was totally different from those of control group. Especially, PM2.5 exposure can change the expression of ARGs in intestinal flora. The ARGs of rpoB2, Tet44, TetW and TetO increased significantly in the PM2.5 inhaled Wistar rats. Controlling air pollution and reducing PM2.5 emissions are crucial to maintain public health.

show abstract

Critical review of antibiotic resistance genes in the atmosphere

Abstract: We conducted a critical review to establish what is known about the sources, characteristics, and dissemination of ARGs in the atmosphere. We identified 52 papers that reported direct measurements of...

Cited by 25 publications

References 111 publications

Low-Level Tetracycline Resistance Gene tet(O)_3 in Campylobacter jejuni

Low-Level Tetracycline Resistance Gene tet(O)_3 in Campylobacter jejuni

Microbiome diversity: A barrier to the environmental spread of antimicrobial resistance?

Can PM2.5 Exposure Change the Expression of ARGs in Intestinal Flora of Wistar Rats?

Contact Info

Product

Resources

About