Critical review on Rumex dentatus L. a strong pharmacophore and the future medicine: Pharmacology, phytochemical analysis and traditional uses

Khaliq, Tahirah; Akhter, Sabiyah; Sultan, Phalisteen; Hassan, Qazi Parvaiz

doi:10.1016/j.heliyon.2023.e14159

Cited by 7 publications

(3 citation statements)

References 75 publications

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“…Studies have shown that another compound, quercetin, reduces tumor weight by targeting VEGFR2 through the Akt/mTOR/P70S6K signaling pathway 30 It also inhibits the Wnt/b-catenin signaling pathway by downregulating target genes, including c-Myc, Cyclin-D1, and TCF4. In addition, endocrocin is reported to have anticancer properties, although there is a lack of available data about the possible mechanisms of its action 31 . Based on the in silico studies, we assumed that these 4 compounds could have important contributions to the overall promising anticancer properties of RO extract.…”

Section: Discussionmentioning

confidence: 99%

5-fluorouracil and Rumex obtusifolius extract combination trigger A549 cancer cell apoptosis: uncovering PI3K/Akt inhibition by in vitro and in silico approaches

Ginovyan,

Javrushyan,

Hovhannisyan

et al. 2024

Sci Rep

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The continuous increase in cancer rates, failure of conventional chemotherapies to control the disease, and excessive toxicity of chemotherapies clearly demand alternative approaches. Natural products contain many constituents that can act on various bodily targets to induce pharmacodynamic responses. This study aimed to explore the combined anticancer effects of Rumex obtusifolius (RO) extract and the chemotherapeutic agent 5-fluorouracil (5-FU) on specific molecular targets involved in cancer progression. By focusing on the PI3K/Akt signaling pathway and its related components, such as cytokines, growth factors (TNFa, VEGFa), and enzymes (Arginase, NOS, COX-2, MMP-2), this research sought to elucidate the molecular mechanisms underlying the anticancer effects of RO extract, both independently and in combination with 5-FU, in non-small lung adenocarcinoma A549 cells. The study also investigated the potential interactions of compounds identified by HPLC/MS/MS of RO on PI3K/Akt in the active site pocket through an in silico analysis. The ultimate goal was to identify potent therapeutic combinations that effectively inhibit, prevent or delay cancer development with minimal side effects. The results revealed that the combined treatment of 5-FU and RO demonstrated a significant reduction in TNFa levels, comparable to the effect observed with RO alone. RO modulated the PI3K/Akt pathway, influencing the phosphorylated and total amounts of these proteins during the combined treatment. Notably, COX-2, a key player in inflammatory processes, substantially decreased with the combination treatment. Caspase-3 activity, indicative of apoptosis, increased by 1.8 times in the combined treatment compared to separate treatments. In addition, the in silico analyses explored the binding affinities and interactions of RO's major phytochemicals with intracellular targets, revealing a high affinity for PI3K and Akt. These findings suggest that the combined treatment induces apoptosis in A549 cells by regulating the PI3K/Akt pathway.

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Section: Discussionmentioning

confidence: 99%

5-fluorouracil and Rumex obtusifolius extract combination trigger A549 cancer cell apoptosis: uncovering PI3K/Akt inhibition by in vitro and in silico approaches

Ginovyan,

Javrushyan,

Hovhannisyan

et al. 2024

Sci Rep

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“…For example, anti-inflammatory agents are used to treat COVID-19 as well as neuroinflammation and also have anti-carcinogenic as well as diabetes-control properties ( Kumar et al, 2023 , Merecz-Sadowska et al, 2023 ; Perico et al, 2022 ). Hence, various kinds of plant sources such as Aerva lanata flower, Ficus religiosa , Kaempferia rotunda Linn., Lavandula dentata L., Rosmarinus officinalis L., Rumex dentatus L., Teucrium multicaule , Tripleurospermum limosum etc., are used for different (medicinal/pharmaceutical) purposes like anti-inflammatory, antioxidant activity, antifertility, antifungal, in vitro biofilm inhibition of bacteria, antifungal, antidiarrheal, and so on ( Baliyan et al, 2022 , Brindhadevi et al, 2023 , Chen et al, 2022a , El Abdali et al, 2022 , Ersoy et al, 2023 , Ibrahim et al, 2022 , Khaliq et al, 2023 , Sahoo et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis, GC–MS profile and determination of antibacterial, antifungal, anti-inflammatory, antioxidant activities of peel and seeds extracts (chloroform and ethyl acetate) of Tamarindus indica L

Tavanappanavar,

Mulla,

Shekhar Seth

et al. 2024

Saudi Journal of Biological Sciences

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“…Pharmacological information shows that Emodin has a wide range of biological activities, including antibacterial, antiviral, and anticancer effects, and its effect on anti-biofilm microbial activity has also been reported. Moreover, Emodin has been found to be non-toxic to mammalian cells at therapeutic concentrations, which is an important consideration for clinical [ 18 – 20 ]. To overcome these adverse effects, scientists have suggested the use of biological nano-biocarriers.…”

Section: Introductionmentioning

confidence: 99%

Periodontal ligament stem cell-derived exosome-loaded Emodin mediated antimicrobial photodynamic therapy against cariogenic bacteria

Pourhajibagher,

Bahador

2024

BMC Oral Health

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Background This study was conducted to investigate the efficiency of periodontal ligament (PDL) stem cell-derived exosome-loaded Emodin (Emo@PDL-Exo) in antimicrobial photodynamic therapy (aPDT) on Streptococcus mutans and Lactobacillus acidophilus as the cariogenic bacteria. Materials and methods After isolating and characterizing PDL-Exo, the study proceeded to prepare and verify the presence of Emo@PDL-Exo. The antimicrobial effect, anti-biofilm activity, and anti-metabolic potency of Emo, PDL-Exo, and Emo@PDL-Exo were then evaluated with and without irradiation of blue laser at a wavelength of 405 ± 10 nm with an output intensity of 150 mW/cm2 for a duration of 60 s. In addition, the study assessed the binding affinity of Emodin with GtfB and SlpA proteins using in silico molecular docking. Eventually, the study examined the generation of endogenous reactive oxygen species (ROS) and changes in the gene expression levels of gelE and sprE. Results The study found that using Emo@PDL-Exo-mediated aPDT resulted in a significant decrease in L. acidophilus and S. mutans by 4.90 ± 0.36 and 5.07 log10 CFU/mL, respectively (P < 0.05). The study found that using Emo@PDL-Exo for aPDT significantly reduced L. acidophilus and S. mutans biofilms by 44.7% and 50.4%, respectively, compared to untreated biofilms in the control group (P < 0.05). Additionally, the metabolic activity of L. acidophilus and S. mutans decreased by 58.3% and 71.2%, respectively (P < 0.05). The molecular docking analysis showed strong binding affinities of Emodin with SlpA and GtfB proteins, with docking scores of -7.4 and -8.2 kcal/mol, respectively. The study also found that the aPDT using Emo@PDL-Exo group resulted in the most significant reduction in gene expression of slpA and gtfB, with a decrease of 4.2- and 5.6-folds, respectively, compared to the control group (P < 0.05), likely due to the increased generation of endogenous ROS. Discussion The study showed that aPDT using Emo@PDL-Exo can effectively reduce the cell viability, biofilm activity, and metabolic potency of S. mutans and L. acidophilus. aPDT also significantly reduced the expression levels of gtfB and slpA mRNA due to the increased endogenous ROS generation. The findings suggest that Emo@PDL-Exo-mediated aPDT could be a promising antimicrobial approach against cariogenic microorganisms.

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Critical review on Rumex dentatus L. a strong pharmacophore and the future medicine: Pharmacology, phytochemical analysis and traditional uses

Cited by 7 publications

References 75 publications

5-fluorouracil and Rumex obtusifolius extract combination trigger A549 cancer cell apoptosis: uncovering PI3K/Akt inhibition by in vitro and in silico approaches

5-fluorouracil and Rumex obtusifolius extract combination trigger A549 cancer cell apoptosis: uncovering PI3K/Akt inhibition by in vitro and in silico approaches

Phytochemical analysis, GC–MS profile and determination of antibacterial, antifungal, anti-inflammatory, antioxidant activities of peel and seeds extracts (chloroform and ethyl acetate) of Tamarindus indica L

Periodontal ligament stem cell-derived exosome-loaded Emodin mediated antimicrobial photodynamic therapy against cariogenic bacteria

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