Critical Role of Monooxygenase in Biodegradation of 2,4,6-Trinitrotoluene by Buttiauxella sp. S19-1

Xu, Mingqiang; Li, He; Wu, Ming; Cui, Xiyan; Liu, Dong; Adomako-Bonsu, Amma G.; Geng, Min; Guan, Xiong; Guo, Liquan; Maser, Edmund

doi:10.3390/molecules28041969

Cited by 6 publications

(1 citation statement)

References 42 publications

(61 reference statements)

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“…Even at low concentrations, TNT exhibits toxic and mutagenic effects on various organisms, from microbes to humans (Lachance et al, 2004), causing rashes, toxic hepatitis, dermatitis, cyanosis, sneezing, cough, peripheral neuritis, cataracts, muscle pain, aplastic anemia and kidney damage (Kalderis et al, 2008). Xu et al (2023) emphasized TNT's high water solubility, which provokes its absorbance by aquatic organisms and poses a significant threat to the ecosystem and the health of seafood consumers.…”

Section: Introductionmentioning

confidence: 99%

Simulating 2,4,6-trinitrotoluene (TNT) elimination in a pond inhabited by freshwater algae of the Rhizoclonium genus

Khromykh,

Marenkov,

Sharamok

et al. 2023

Regul. Mech. Biosyst.

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Military operations over large areas of Ukraine lead to release of explosives and their derivatives into the environment with subsequent accumulation in natural and artificial water bodies, which unwittingly serve as reservoirs for collecting pollutants from the catchment area. The need to restore aquatic ecosystems dictates the search for efficient, cost-effective and environmentally friendly methods for the elimination of explosives, which corresponds to the processes of biological treatment. In this work, we examined the ability of common freshwater algae of the genus Rhizoclonium to detoxify 2,4,6-trinitrotoluene (TNT) under model conditions of water pollution (at a TNT concentration of 100 mg/L). The exposure time of the algae to TNT was 48 hours, during which the content of TNT and nitrites in the aqueous medium was monitored, as well as the content of chlorophyll and the activity of glutathione S-transferase in plant tissues. 2,4,6-trinitrotoluene was extracted from the aqueous medium with toluene, followed by separation in a separatory funnel, removal of residual water with sodium sulfate, and reduction of the extract volume using a rotary evaporator. The decrease in the concentration of TNT, established by GC-MS technique, was 66.4% by the end of the experiment, while the content of nitrites increased almost 15-fold. In the algae cells, a threefold increase in the enzymatic activity was observed already in the second hour of exposure, followed by a gradual decrease and maintenance at a level of 50% of the control until the end of the experiment. The total chlorophyll content increased significantly from the sixth hour of exposure to the end of the experiment due to an increase in the content of chlorophyll b. The results obtained indicate the efficient biodegradation process and prospects of using algae of the genus Rhizoclonium for cleaning water bodies contaminated with TNT.

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Section: Introductionmentioning

confidence: 99%

Simulating 2,4,6-trinitrotoluene (TNT) elimination in a pond inhabited by freshwater algae of the Rhizoclonium genus

Khromykh,

Marenkov,

Sharamok

et al. 2023

Regul. Mech. Biosyst.

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Biodegradation of 2,4,6-Trinitrotoluene (TNT) by the Microbes and Their Synergistic Interactions

Lamba,

Bhardwaj,

Anand

et al. 2024

Energy, Environment, and Sustainability

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Role of Extremophiles in Biodegradation of Emerging Pollutants

Chia,

Hadibarata,

Jusoh

et al. 2024

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Emerging pollutants, also referred to as emerging contaminants, are substances that have recently been recognized or are gaining attention due to their potential adverse impacts on the environment, human health, or ecosystems. These pollutants present a significant threat to both environmental and human well-being and are challenging to eliminate using conventional remediation methods. Extremophiles, organisms adapted to extreme environmental conditions like high or low temperatures, high pressure, and elevated salt concentrations, play a crucial role in this context. They produce a diverse array of enzymes capable of breaking down complex organic compounds, some of which remain stable and functional even in harsh environmental conditions, making extremophiles well-suited for use in bioremediation applications. Numerous studies have demonstrated the capability of extremophiles to degrade various pollutants, including toxic solvents, heavy metals, and industrial chemicals. Halophilic archaea, a type of extremophile, have particularly shown promise in degrading emerging contaminants in salt marsh sediments. Despite their potential, there are challenges associated with using extremophiles in bioremediation, such as the limited availability of extremophilic microorganisms capable of degrading specific pollutants and a reduction in enzyme stability when operating outside their optimum range. Nevertheless, ongoing research in this field is anticipated to result in the development of new and innovative bioremediation strategies for effectively removing emerging pollutants from the environment.

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Critical Role of Monooxygenase in Biodegradation of 2,4,6-Trinitrotoluene by Buttiauxella sp. S19-1

Cited by 6 publications

References 42 publications

Simulating 2,4,6-trinitrotoluene (TNT) elimination in a pond inhabited by freshwater algae of the Rhizoclonium genus

Simulating 2,4,6-trinitrotoluene (TNT) elimination in a pond inhabited by freshwater algae of the Rhizoclonium genus

Biodegradation of 2,4,6-Trinitrotoluene (TNT) by the Microbes and Their Synergistic Interactions

Role of Extremophiles in Biodegradation of Emerging Pollutants

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