Critical role of Neuropeptide FF receptor 2 in the regulation of energy balance and glucose homeostasis revealed in mice

Zhang, L.; Lee, I.; Lin, Shu; Herzog, Herbert

doi:10.1016/j.orcp.2012.08.035

Cited by 2 publications

(4 citation statements)

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“…NPFF exerted dual actions in the parabrachial nucleus to modulate food intake in rats (36). In addition, lacking of NPFFR2 may lead to reduced adiposity and decreased food intake in both male and female mice but especially in males (37). The heritable variability of NPFFR2 is closely associated with an enhanced obesity risk; therefore, NPFFR2 plays a key role in obesity predisposition (72).…”

Section: Discussionmentioning

confidence: 99%

“…The heritable variability of NPFFR2 is closely associated with an enhanced obesity risk; therefore, NPFFR2 plays a key role in obesity predisposition (72). In contrast to rodents, human NPFF was found to inhibit catecholamine-induced lipolysis in human fat cells via NPFFR2 activation (37). The GnIH (RFRP-3), a paralog of NPFF, significantly stimulated food intake in chick (38, 39).…”

Section: Discussionmentioning

confidence: 99%

“…Although injection of lower doses of the NPFF peptide (5–10 nM) can reduce food intake, higher doses of NPFF increase food intake (36). Prior evidence has confirmed that loss of NPFFR2 in male rats is associated with a significantly decreased food intake (37). On the other hand, gonadotropin-inhibitory hormone (GnIH), a paralog of NPFF, was also proved to regulate food intake via NPFFR1 in chicks and jerboas (38–40).…”

Section: Introductionmentioning

confidence: 88%

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Evidence for the Direct Effect of the NPFF Peptide on the Expression of Feeding-Related Factors in Spotted Sea Bass (Lateolabrax maculatus)

Wen

et al. 2019

Front. Endocrinol.

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Neuropeptide FF (NPFF) is a family member of RF-amide peptides, which are suggested to be involved in the control of vertebrate feeding behavior. However, little is known about the effect of the NPFF peptide on feeding-related processes in basal vertebrates. In this study, four full-length cDNAs, npff , npffr1, npffr2-1 , and npffr2-2 , were cloned from spotted sea bass and characterized. The conserved NPFF peptide is biologically active because it functionally interacts with different receptors expressed in cultured eukaryotic cells to enhance CRE promoter activity. Tissue distribution analysis showed that the highest npff mRNA expression occurred in the telencephalon, hypothalamus, medulla, gonad and muscle, but the npffr s mRNAs were mainly distributed within the central nervous system (CNS). In situ hybridization (ISH) detected npff -expressing cells in several specific regions ranging across the telencephalon and midbrain to the hypothalamus. Incubation of the spotted sea bass conserved NPFF peptide significantly increased the expression of orexin ( orx ) and neuropeptide Y ( npy ) mRNA and decreased the expression of leptin ( lep ), somatostatin ( ss ), and cholecystokinin ( cck ) mRNA in brain cells. Similarly, the conserved NPFF peptide also heightened the expression of gastrin ( gas ), ghrelin ( ghrl ), and motilin ( mtl ) mRNA and significantly reduced the expression of cck mRNA in the intestine and stomach. Taken together, these data suggest that the NPFF peptide may play a stimulating role in regulating feeding-related processes in spotted sea bass.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

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Evidence for the Direct Effect of the NPFF Peptide on the Expression of Feeding-Related Factors in Spotted Sea Bass (Lateolabrax maculatus)

Wen

et al. 2019

Front. Endocrinol.

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“…The anorexigenic properties of neuropeptide FF (NPFF) in rodents and chicks have been long known [ 1 , 2 , 3 , 4 ]. It has also been recently discovered that NPFF receptor 2 (NPFFR2) is also a crucial regulator of diet-induced adaptative thermogenesis in mice [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model

Torz

Niss

Lundh

et al. 2022

IJMS

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Restoring the control of food intake is the key to obesity management and prevention. The arcuate nucleus (ARC) of the hypothalamus is extensively being studied as a potential anti-obesity target. Animal studies showed that neuropeptide FF (NPFF) reduces food intake by its action in neuropeptide Y (NPY) neurons of the hypothalamic ARC, but the detailed mode of action observed in human neurons is missing, due to the lack of a human-neuron-based model for pharmacology testing. Here, we validated and utilized a human-neural-stem-cell-based (hNSC) model of ARC to test the effects of NPFF on cellular pathways and neuronal activity. We found that in the human neurons, decreased cAMP levels by NPFF resulted in a reduced rate of cytoplasmic calcium oscillations, indicating an inhibition of ARC NPY neurons. This suggests the therapeutic potential of NPFFR2 in obesity. In addition, we demonstrate the use of human-stem-cell-derived neurons in pharmacological applications and the potential of this model to address functional aspects of human hypothalamic neurons.

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Critical role of Neuropeptide FF receptor 2 in the regulation of energy balance and glucose homeostasis revealed in mice

Cited by 2 publications

References 0 publications

Evidence for the Direct Effect of the NPFF Peptide on the Expression of Feeding-Related Factors in Spotted Sea Bass (Lateolabrax maculatus)

Evidence for the Direct Effect of the NPFF Peptide on the Expression of Feeding-Related Factors in Spotted Sea Bass (Lateolabrax maculatus)

NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model

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