Critical Role of p53 and K-ras in the Diagnosis of Early Colorectal Cancer: a One-year, Single-center Analysis

Abstract

Background: Colorectal cancer (CRC) is strongly associated with colorectal polyps, which has become the third most common cancer in China. In the present study, we revealed the susceptible population and risk factors of colorectal polyps, and analyzed the expression of Ki-67, p53 and K-ras in the intestinal mucosa of patients with colorectal polyps in order to explore their significance in the detection and prognosis of CRC at an early stage.Materials and Methods: Total 801 cases of colorectal polyps were collected during endoscopic resection including endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD). Expression of Ki-67, p53 and K-ras in the intestinal mucosa was detected by immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Histological analysis was performed by Hematoxylin and eosin (HE) staining. Categorical variables were compared by one-way ANOVA, Pearson test, Spearman test, Kruskal-Wallis test and analysis of regression.Results: Of all patients with colorectal polyps, 90.76% of patients (n = 727) were ≥ 50 years old. 530 cases (66.17%) were males compared with 271 females (33.83%) in all 801 cases. More importantly, 1.03% patients (n = 7) underwent polypectomy and histological examination was confirmed to be the early stage of CRC. The expression of p53 was found to be significantly decreased, while K-ras was increased in tumor tissues of CRC compared with that in hyperplastic polyps and healthy controls.Conclusions: 1.03% patients (n = 7) underwent polypectomy was confirmed to be the early stage of CRC. Histological analysis for expression of p53 and K-ras can guarantee to screen the early stage of CRC.

Single Investigation of the Role of APC2 in Colorectal Cancer

“…In previous studies, there was a close connection between the P53 signaling pathway and the Kras protein signaling pathway [13.14.15]. This pathway is particularly important in colorectal cancer cell lines [16].…”

Hsa_circ_001659 serves as a diagnostic and prognostic biomarker for colorectal cancer

“…Identi cation of novel serum biomarkers is an important goal in the diagnosis of cancer, especially for detection and screening in early-stage cancer [2,17,18]. Currently, the repertoire of well-documented biomarkers for CRC early diagnosis is quite limited.…”

Analysis of prognosis, genome, microbiome, and microbial metabolome in different sites of colorectal cancer

“…The analysis of these differential gene expressions provides further research direction for the pathogenesis and prognosis of different CRC sites. The expression levels of some genes, for instance, K-RAS, N-RAS, BRAF, p53, Ki-67, and MSI (microsatellite instability) classification have been used to predict the prognosis of CRC [45][46][47][48]. The search for more gene targets will contribute to accurately determine the treatment regimen decision and prognosis evaluation of CRC.…”